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Start Over Author "Jiang, Chen" Topic gene therapy Journal plos one
2 results on '"Jiang, Chen"'

1. Cytoplasmic and/or Nuclear Expression of β-Catenin Correlate with Poor Prognosis and Unfavorable Clinicopathological Factors in Hepatocellular Carcinoma: A Meta-Analysis

Author

Hong Yu, Jiang Chen, Xiao Liang, Yuelong Liang, Hui Lin, Jiliang Shen, Jinghua Liu, Rui Ma, Renan Jin, and Xiujun Cai

Subjects
Oncology, Liver Cirrhosis, Cytoplasm, lcsh:Medicine, Gene Expression, Metastasis, Medicine and Health Sciences, Odds Ratio, lcsh:Science, beta Catenin, Multidisciplinary, Effector, Liver Diseases, Liver Neoplasms, Wnt signaling pathway, Gene Therapy, Tumor Burden, Hepatocellular carcinoma, Cluster Trials, Clinical Trial Reporting, alpha-Fetoproteins, Signal transduction, Research Article, medicine.medical_specialty, Carcinoma, Hepatocellular, Gastroenterology and Hepatology, Biology, Internal medicine, medicine, Biomarkers, Tumor, Humans, Clinical Trials, Molecular Biology Techniques, Molecular Biology, Neoplasm Staging, Clinical Genetics, Cell Nucleus, lcsh:R, Biology and Life Sciences, Hepatocellular Carcinoma, HCCS, medicine.disease, digestive system diseases, Catenin, Cancer research, lcsh:Q, Non-Randomized Controlled Trials, Clinical Medicine, Neoplasm Grading, Neoplasm Recurrence, Local
Abstract

BACKGROUND: The β-catenin is an important effector in WNT/β-catenin signaling pathway, which exerts a crucial role in the development and progression of hepatocellular carcinoma (HCC). Some researchers have suggested that the overexpression of β-catenin in cytoplasm and/or nucleus was closely correlated to metastasis, poor differentiation and malignant phenotype of HCC while some other researchers hold opposite point. So far, no consensus was obtained on the prognostic and clinicopathological significance of cytoplasmic/nuclear β-catenin overexpression for HCCs. METHODS: Systematic strategies were applied to search eligible studies in all available databases. Subgroup analyses, sensitivity analyses and multivariate analysis were performed. In this meta-analysis, we utilized either fixed- or random-effects model to calculate the pooled odds ratios (OR) and its 95% confidence intervals (CI). RESULTS: A total of 22 studies containing 2334 cases were enrolled in this meta-analysis. Pooled data suggested that accumulation of β-catenin in cytoplasm and/or nucleus significantly correlated with poor 1-, 3- and 5-year OS and RFS. Moreover, nuclear accumulation combined with cytoplasmic accumulation of β-catenin tended to be associated with dismal metastasis and vascular invasion while cytoplasmic or nuclear expression alone showed no significant effect. Besides, no significant association was observed between cytoplasmic and/or nuclear β-catenin expression and poor differentiation grade, advanced TNM stage, liver cirrhosis, tumor size, tumor encapsulation, AFP and etiologies. Additional subgroup analysis by origin suggested that the prognostic value and clinicopathological significance of cytoplasmic and/or nuclear β-catenin expression was more validated in Asian population. Multivariate analyses of factors showed that cytoplasmic and/or nuclear β-catenin expression, as well as TNM stage, metastasis and tumor size, was an independent risk factors for OS and RFS. CONCLUSIONS: Cytoplasmic and/or nuclear accumulation of β-catenin, as an independent prognostic factor, significantly associated with poor prognosis and deeper invasion of HCC, and could serve as a valuable prognostic predictor for HCC.

Published
2014

2. Targeting Caspase-3 as Dual Therapeutic Benefits by RNAi Facilitating Brain-Targeted Nanoparticles in a Rat Model of Parkinson’s Disease

Author

Liu, Yang, Guo, Yubo, An, Sai, Kuang, Yuyang, He, Xi, Ma, Haojun, Li, Jianfeng, Lv, Jing, Zhang, Ning, and Jiang, Chen

Subjects
CASPASE inhibitors, RNA interference, NANOMEDICINE, PARKINSON'S disease, APOPTOSIS, INFLAMMATION, LABORATORY rats
Abstract

The activation of caspase-3 is an important hallmark in Parkinson’s disease. It could induce neuron death by apoptosis and microglia activation by inflammation. As a result, inhibition the activation of caspase-3 would exert synergistic dual effect in brain in order to prevent the progress of Parkinson’s disease. Silencing caspase-3 genes by RNA interference could inhibit the activation of caspase-3. We developed a brain-targeted gene delivery system based on non-viral gene vector, dendrigraft poly-L-lysines. A rabies virus glycoprotein peptide with 29 amino-acid linked to dendrigraft poly-L-lysines could render gene vectors the ability to get across the blood brain barrier by specific receptor mediated transcytosis. The resultant brain-targeted vector was complexed with caspase-3 short hairpin RNA coding plasmid DNA, yielding nanoparticles. In vivo imaging analysis indicated the targeted nanoparticles could accumulate in brain more efficiently than non-targeted ones. A multiple dosing regimen by weekly intravenous administration of the nanoparticles could reduce activated casapse-3 levels, significantly improve locomotor activity and rescue dopaminergic neuronal loss and in Parkinson’s disease rats’ brain. These results indicated the rabies virus glycoprotein peptide modified brain-targeted nanoparticles were promising gene delivery system for RNA interference to achieve anti-apoptotic and anti-inflammation synergistic therapeutic effects by down-regulation the expression and activation of caspase-3. [ABSTRACT FROM AUTHOR]

Published
2013
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