1. Performance of Four Transport and Storage Systems for Molecular Detection of Multidrug-Resistant Tuberculosis
- Author
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Anandi Martin, Rina Andrianiavomikotroka, Lucilaine Ferrazoli, Peter Vandamme, Sarman Singh, Belén Rocío Imperiale, Marie Sylvianne Rabodoarivelo, Voahangy Rasolofo, Angela Pires Brandão, Juan Carlos Palomino, Nora Morcillo, and Parveen Kumar
- Subjects
MTBDRPLUS ASSAY ,Genotyping Techniques ,Antitubercular Agents ,Ciencias de la Salud ,lcsh:Medicine ,Transportation ,Drug resistance ,Polymerase Chain Reaction ,Ribotyping ,Drug Resistance, Multiple, Bacterial ,Tuberculosis, Multidrug-Resistant ,Medicine and Health Sciences ,Molecular diagnostic techniques ,TRANSPORT AND STORAGE SYSTEM ,lcsh:Science ,Multidisciplinary ,biology ,DRUG RESISTANT M. TUBERCULOSIS ,Bacterial Typing Techniques ,Molecular Diagnostic Techniques ,purl.org/becyt/ford/3 [https] ,MYCOBACTERIUM-TUBERCULOSIS ,Brazil ,Research Article ,DNA, Bacterial ,Paper ,CIENCIAS MÉDICAS Y DE LA SALUD ,TISSUES ,Tuberculosis ,Genotype ,Preservation, Biological ,Argentina ,India ,Computational biology ,Microbial Sensitivity Tests ,Reference laboratory ,MOLECULAR DRUG RESISTANCE DETECTION ,Sensitivity and Specificity ,Microbiology ,Specimen Handling ,Mycobacterium tuberculosis ,purl.org/becyt/ford/3.3 [https] ,Postal service ,medicine ,Humans ,Postal Service ,Retrospective Studies ,RIFAMPIN ,GenoType MTBDRplus ,Bacteriological Techniques ,IDENTIFICATION ,Ethanol ,STRAINS ,lcsh:R ,CARDS ,Sputum ,Extensively drug-resistant tuberculosis ,DNA ,biology.organism_classification ,medicine.disease ,SPECIMENS ,Enfermedades Infecciosas ,Multiple drug resistance ,lcsh:Q ,Glass ,Filtration - Abstract
Background Detection of drug-resistant tuberculosis is essential for the control of the disease but it is often hampered by the limitation of transport and storage of samples from remote locations to the reference laboratory. We performed a retrospective field study to evaluate the performance of four supports enabling the transport and storage of samples to be used for molecular detection of drug resistance using the GenoType MTBDRplus. Methods Two hundred Mycobacterium tuberculosis strains were selected and spotted on slides, FTA cards, GenoCards, and in ethanol. GenoType MTBDRplus was subsequently performed with the DNA extracted from these supports. Sensitivity and specificity were calculated and compared to the results obtained by drug susceptibility testing. Results For all supports, the overall sensitivity and specificity for detection of resistance to RIF was between 95% and 100%, and for INH between 95% and 98%. Conclusion The four transport and storage supports showed a good sensitivity and specificity for the detection of resistance to RIF and INH in M. tuberculosis strains using the GenoType MTBDRplus. These supports can be maintained at room temperature and could represent an important alternative cost-effective method useful for rapid molecular detection of drugresistant TB in low-resource settings. Fil: Rabodoarivelo, Marie Sylvianne. University of Ghent; Bélgica Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina Fil: Andrianiavomikotroka, Rina. Institut Pasteur de Madagascar; Madagascar Fil: Brandao, Angela. Fundación Oswaldo Cruz; Brasil Fil: Kumar, Parveen. All India Institute of Medical Sciences; India Fil: Singh, Sarman. All India Institute of Medical Sciences; India Fil: Ferrazoli, Lucilaine. Instituto Adolfo Lutz Núcleo de Tuberculose e Micobacteriosis Sao Paulo; Brasil Fil: Morcillo, Nora. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina Fil: Rasolofo, Voahangy. Institut Pasteur de Madagascar; Madagascar Fil: Palomino, Juan Carlos. University of Ghent; Bélgica Fil: Vandamme, Peter. University of Ghent; Bélgica Fil: Martin, Anandi. University of Ghent; Bélgica
- Published
- 2015