1. Therapeutic Effects of Bupleurum Polysaccharides in Streptozotocin Induced Diabetic Mice.
- Author
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Pan L, Weng H, Li H, Liu Z, Xu Y, Zhou C, Lu X, Su X, Zhang Y, and Chen D
- Subjects
- Animals, Anti-Inflammatory Agents chemistry, Anti-Inflammatory Agents therapeutic use, Antioxidants chemistry, Antioxidants therapeutic use, Blood Glucose metabolism, Body Weight drug effects, Diabetes Mellitus, Experimental blood, Diabetes Mellitus, Experimental metabolism, Diabetes Mellitus, Experimental pathology, Hypoglycemic Agents chemistry, Insulin blood, Insulin metabolism, Male, Mice, Mice, Inbred C57BL, Pancreas drug effects, Pancreas metabolism, Pancreas pathology, Polysaccharides chemistry, Bupleurum chemistry, Diabetes Mellitus, Experimental drug therapy, Hypoglycemic Agents therapeutic use, Polysaccharides therapeutic use
- Abstract
Diabetes mellitus is related to low-grade chronic inflammation and oxidative stress. Bupleurum Polysaccharides (BPs), isolated from Bupleurum smithii var. parvifolium has anti-inflammatory and anti-oxidative properties. However, little is known about its therapeutic effects on diabetes. In this experiment, the effects of BPs on alleviation of diabetes and the underlying mechanisms were investigated. Diabetic mice model was established via successive intraperitoneal injections of streptozotocin (100 mg/kg body weight) for two days. Mice with blood glucose levels higher than 16.8mmol/L were selected for experiments. The diabetic mice were orally administered with BPs (30 and 60 mg/kg) once a day for 35 days. BPs not only significantly decreased levels of blood glucose, but also increased those of serum insulin and liver glycogen in diabetic mice compared to model mice. Additionally, BPs adminstration improved the insulin expression and suppressed the apoptosis in pancreas of the diabetic mice. Histopathological observations further demonstrated that BPs protected the pancreas and liver from oxidative and inflammatory damages. These results suggest that BPs protect pancreatic β cells and liver hepatocytes and ameliorate diabetes, which is associated with its anti-oxidative and anti-inflammatory properties.
- Published
- 2015
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