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Your search keyword '"An, Minghui"' showing total 3 results
3 results on '"An, Minghui"'

1. Evaluation of reference genes for quantitative real-time PCR normalization in the scarab beetle Holotrichia oblita

Author

Lulu Lin, Mingjing Qu, Minghui Xie, Zhong Yongzhi, Weihua Su, Guangling Zhang, Wan-Li Ni, and Haoliang Chen

Subjects
0106 biological sciences, Male, Life Cycles, Gene Expression, 01 natural sciences, Biochemistry, law.invention, Larvae, Animal Wings, law, Reference genes, Gene expression, Abdomen, Medicine and Health Sciences, Animal Anatomy, Polymerase chain reaction, Regulation of gene expression, 0303 health sciences, Multidisciplinary, Temperature, Eukaryota, Gene Expression Regulation, Developmental, Agriculture, Reference Standards, Insects, Coleoptera, Insect Proteins, Medicine, Female, Anatomy, Agrochemicals, Research Article, Normalization (statistics), Crops, Agricultural, Arthropoda, Photoperiod, Science, Computational biology, Biology, Holotrichia oblita, Real-Time Polymerase Chain Reaction, Odorant Binding Proteins, 03 medical and health sciences, Genetics, Animals, Pesticides, Gene, 030304 developmental biology, Scarabaeidae, Gene Expression Profiling, Organisms, Biology and Life Sciences, Proteins, Animal Antennae, biology.organism_classification, Invertebrates, 010602 entomology, Pest Control, Zoology, Entomology, Developmental Biology
Abstract

Quantitative real-time polymerase chain reaction (qPT-PCR) is commonly used to analyze gene expression, however, the accuracy of the normalized results is affected by the expression stability of reference genes. Holotrichia oblita (Coleoptera: Scarabaeidae) causes serious damage to crops. Reliable reference genes in H. oblita are needed for qRT-PCR analysis. Therefore, we evaluated 13 reference genes under biotic and abiotic conditions. RefFinder provided a comprehensive stability ranking, and geNorm suggested the optimal number of reference genes for normalization. RPL13a and RPL18 were the most suitable reference genes for developmental stages, tissues, and temperature treatments; RPL13a and RPS3 were the most suitable for pesticide and photoperiod treatments; RPS18 and RPL18 were the most suitable for the two sexes. We validated the normalized results using odorant-binding protein genes as target genes in different tissues. Compared with the selected suitable reference genes, the expression of OBP1 in antennae, abdomen, and wings, and OBP2 in antennae and wings were overestimated due to the instability of ACTb. These results identified several reliable reference genes in H. oblita for normalization, and are valuable for future molecular studies.

Published
2020

2. Transcriptome analysis of immune genes in peripheral blood mononuclear cells of young foals and adult horses

Author

Rebecca L. Tallmadge, Minghui Wang, Maria Julia B. Felippe, and Qi Sun

Subjects
0301 basic medicine, Chemokine, B Cells, lcsh:Medicine, Gene Expression, Complement receptor, Immune Receptors, Biochemistry, White Blood Cells, 0302 clinical medicine, Animal Cells, Medicine and Health Sciences, lcsh:Science, Receptor, Toll-like Receptors, Mammals, Immunity, Cellular, Multidisciplinary, Immune System Proteins, T Cells, Eukaryota, Genomics, medicine.anatomical_structure, Vertebrates, Cellular Types, Transcriptome Analysis, Research Article, Signal Transduction, Immune Cells, Equines, Immunology, Biology, Natural killer cell, 03 medical and health sciences, Immune system, medicine, Genetics, Animals, Horses, Antibody-Producing Cells, B cell, Innate immune system, Blood Cells, Gene Expression Profiling, lcsh:R, Organisms, Biology and Life Sciences, Proteins, Computational Biology, Cell Biology, Genome Analysis, T Cell Receptors, 030104 developmental biology, Immune System, TLR3, Amniotes, biology.protein, Leukocytes, Mononuclear, lcsh:Q, 030215 immunology
Abstract

During the neonatal period, the ability to generate immune effector and memory responses to vaccines or pathogens is often questioned. This study was undertaken to obtain a global view of the natural differences in the expression of immune genes early in life. Our hypothesis was that transcriptome analyses of peripheral blood mononuclear cells (PBMCs) of foals (on day 1 and day 42 after birth) and adult horses would show differential gene expression profiles that characterize natural immune processes. Gene ontology enrichment analysis provided assessment of biological processes affected by age, and a list of 897 genes with ≥2 fold higher (p

Published
2017

3. Transcriptome analysis of immune genes in peripheral blood mononuclear cells of young foals and adult horses.

Author

Tallmadge, Rebecca L., Wang, Minghui, Sun, Qi, and Felippe, Maria Julia B.

Subjects
*BLOOD cell physiology, *IMMUNE response, *MONONUCLEAR leukocytes, *GENE ontology, *DNA replication
Abstract

During the neonatal period, the ability to generate immune effector and memory responses to vaccines or pathogens is often questioned. This study was undertaken to obtain a global view of the natural differences in the expression of immune genes early in life. Our hypothesis was that transcriptome analyses of peripheral blood mononuclear cells (PBMCs) of foals (on day 1 and day 42 after birth) and adult horses would show differential gene expression profiles that characterize natural immune processes. Gene ontology enrichment analysis provided assessment of biological processes affected by age, and a list of 897 genes with ≥2 fold higher (p<0.01) expression in day 42 when compared to day 1 foal samples. Up-regulated genes included B cell and T cell receptor diversity genes; DNA replication enzymes; natural killer cell receptors; granzyme B and perforin; complement receptors; immunomodulatory receptors; cell adhesion molecules; and cytokines/chemokines and their receptors. The list of 1,383 genes that had higher (p<0.01) expression on day 1 when compared to day 42 foal samples was populated by genes with roles in innate immunity such as antimicrobial proteins; pathogen recognition receptors; cytokines/chemokines and their receptors; cell adhesion molecules; co-stimulatory molecules; and T cell receptor delta chain. Within the 742 genes with increased expression between day 42 foal and adult samples, B cell immunity was the main biological process (p = 2.4E-04). Novel data on markedly low (p<0.0001) TLR3 gene expression, and high (p≤0.01) expression of IL27, IL13RA1, IREM-1, SIRL-1, and SIRPα on day 1 compared to day 42 foal samples point out potential mechanisms of increased susceptibility to pathogens in early life. The results portray a progression from innate immune gene expression predominance early in life to adaptive immune gene expression increasing with age with a putative overlay of immune suppressing genes in the neonatal phase. These results provide insight to the unique attributes of the equine neonatal and young immune system, and offer many avenues of future investigation. [ABSTRACT FROM AUTHOR]

Published
2018
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