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Your search keyword '"Zhang, Yongxing"' showing total 2 results
Start Over Author "Zhang, Yongxing" Topic amino acid sequence Journal plos one
2 results on '"Zhang, Yongxing"'

1. OsMPH1 regulates plant height and improves grain yield in rice

Author

Jun Liu, Jing Wang, Aobo Huang, Chunsheng Yu, Zhang Yongxing, Zhao Tao, Jianzhong Lin, Liu Bin, and Li Hongyu

Subjects
0106 biological sciences, 0301 basic medicine, Internodes, lcsh:Medicine, Gene Expression, Genetically modified crops, Plant Science, 01 natural sciences, Genetically Modified Plants, Biochemistry, Arabidopsis thaliana, lcsh:Science, Phylogeny, Plant stem, Plant Proteins, Gene knockdown, Multidisciplinary, Plant Stems, Organic Compounds, Genetically Modified Organisms, Plant Anatomy, food and beverages, Plants, Plants, Genetically Modified, Cell biology, Chemistry, Phenotype, Experimental Organism Systems, RNA, Plant, Physical Sciences, RNA Interference, Cellular Structures and Organelles, Plant Cell Walls, Cellular Types, Genetic Engineering, Research Article, Biotechnology, Arabidopsis Thaliana, Plant Cell Biology, Brassica, Biology, Research and Analysis Methods, 03 medical and health sciences, Model Organisms, Cell Walls, Plant and Algal Models, Plant Cells, DNA-binding proteins, Genetics, Gene Regulation, Amino Acid Sequence, Grasses, Gene, Transcription factor, Ethanol, Sequence Analysis, RNA, lcsh:R, fungi, Organic Chemistry, Organisms, Chemical Compounds, Biology and Life Sciences, Proteins, Oryza, Cell Biology, Stem Anatomy, biology.organism_classification, Genetically modified rice, Regulatory Proteins, 030104 developmental biology, Agronomy, Yield (chemistry), Alcohols, lcsh:Q, Plant Biotechnology, Rice, Sequence Alignment, 010606 plant biology & botany, Transcription Factors
Abstract

Plant height is a major trait affecting yield potential in rice. Using a large-scale hybrid transcription factor approach, we identified the novel MYB-like transcription factor OsMPH1 (MYB-like gene of Plant Height 1), which is involved in the regulation of plant height in rice. Overexpression of OsMPH1 leads to increases of plant height and grain yield in rice, while knockdown of OsMPH1 leads to the opposite phenotypes. Microscopy of longitudinal stem sections indicated that a change in internode cell length resulted in the change in plant height. RNA sequencing (RNA-seq) analysis of transgenic rice lines showed that multiple genes related to cell elongation and cell wall synthesis, which are associated with plant height and yield phenotypes, exhibited an altered expression profile. These results imply that OsMPH1 might be involved in specific recognition and signal transduction processes related to plant height and yield formation, providing further insights into the mechanisms underlying the regulation of plant height and providing a candidate gene for the efficient improvement of rice yield.

Published
2017

2. Isoform Switch of Pyruvate Kinase M1 Indeed Occurs but Not to Pyruvate Kinase M2 in Human Tumorigenesis.

Author

Zhan, Cheng, Yan, Li, Wang, Lin, Ma, Jun, Jiang, Wei, Zhang, Yongxing, Shi, Yu, and Wang, Qun

Subjects
PYRUVATE kinase, CARCINOGENESIS, HUMAN genome, AMINO acid sequence, CANCER cells
Abstract

Muscle type of pyruvate kinase (PKM) is one of the key mediators of the Warburg effect and tumor metabolism. Due to alternative splicing, there are at least 12 known isoforms of the PKM gene, of which PKM1 and PKM2 are two major isoforms with only a 23 amino acid sequenced difference but quite different characteristics and functions. It was previously thought the isoform switch from PKM1 to PKM2 resulted in high PKM2 expression in tumors, providing a great advantage to tumor cells. However, this traditional view was challenged by two recent studies; one study claimed that this isoform switch does not occur during the Warburg effect; the other study asserted that the isoform switch is tissue-specific. Here, we re-analyzed the RNA sequencing data of 25 types of human tumors from The Cancer Genome Atlas Data Portal, and confirmed that PKM2 was the major isoform in the tumors and was highly elevated in addition to the entire PKM gene. We further demonstrated that the expression level of PKM1 significantly declined even though there was substantially increased expression of the entire PKM gene. The proportion of PKM1 in total transcript variants also significantly declined in tumors but the proportion of PKM2 did not change accordingly. Therefore, we conclude that the isoform switch of PKM1 does indeed occur, but it switches to other isoforms rather than PKM2. Considering the change in the expression levels of PKM1, PKM2 and the entire PKM gene, we propose that the upregulation of PKM2 is primarily due to elevated transcriptional levels of the entire PKM gene, instead of the isoform switch. [ABSTRACT FROM AUTHOR]

Published
2015
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