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Your search keyword '"Fang LIU"' showing total 4 results
4 results on '"Fang LIU"'

1. Characterization of long noncoding RNA and messenger RNA signatures in melanoma tumorigenesis and metastasis

Author

Ping Han, Feng Jin, Wenliang Fan, Siqi Wang, Fang Liu, Haibo Xu, Mengqi Tu, and Bing Wan

Subjects
0301 basic medicine, Melanomas, Skin Neoplasms, Microarrays, Carcinogenesis, Gene Expression, lcsh:Medicine, Bioinformatics, Biochemistry, Metastasis, Database and Informatics Methods, Basic Cancer Research, Databases, Genetic, Medicine and Health Sciences, Neoplasm Metastasis, lcsh:Science, Melanoma, Skin, Regulation of gene expression, Multidisciplinary, Messenger RNA, Genomics, Genomic Databases, Prognosis, Long non-coding RNA, Nucleic acids, Gene Expression Regulation, Neoplastic, Survival Rate, Bioassays and Physiological Analysis, Cell Transformation, Neoplastic, Oncology, RNA, Long Noncoding, Research Article, Biology, Research and Analysis Methods, 03 medical and health sciences, medicine, Genetics, Humans, RNA, Messenger, Non-coding RNA, neoplasms, Biology and life sciences, Microarray analysis techniques, Gene Expression Profiling, lcsh:R, Cancers and Neoplasms, Computational Biology, Correction, medicine.disease, Genome Analysis, Gene expression profiling, 030104 developmental biology, Biological Databases, Cutaneous melanoma, Cancer research, Long non-coding RNAs, RNA, lcsh:Q, Skin cancer
Abstract

The incidence of melanoma, the most aggressive and life-threatening form of skin cancer, has significantly risen over recent decades. Therefore, it is essential to identify the mechanisms that underlie melanoma tumorigenesis and metastasis and to explore novel and effective melanoma treatment strategies. Accumulating evidence s uggests that aberrantly expressed long noncoding RNAs (lncRNAs) have vital functions in multiple cancers. However, lncRNA functions in melanoma tumorigenesis and metastasis remain unclear. In this study, we investigated lncRNA and messenger RNA (mRNA) expression profiles in primary melanomas, metastatic melanomas and normal skin samples from the Gene Expression Omnibus database. We used GSE15605 as the training set (n = 74) and GSE7553 as the validation set (n = 58). In three comparisons (primary melanoma versus normal skin, metastatic melanoma versus normal skin, and metastatic melanoma versus primary melanoma), 178, 295 and 48 lncRNAs and 847, 1758, and 295 mRNAs were aberrantly expressed, respectively. We performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses to examine the differentially expressed mRNAs, and potential core lncRNAs were predicted by lncRNA-mRNA co-expression networks. Based on our results, 15 lncRNAs and 144 mRNAs were significantly associated with melanoma tumorigenesis and metastasis. A subsequent analysis suggested a critical role for a five-lncRNA signature during melanoma tumorigenesis and metastasis. Low expression of U47924.27 was significantly associated with decreased survival of patients with melanoma. To the best of our knowledge, this study is the first to explore the expression patterns of lncRNAs and mRNAs during melanoma tumorigenesis and metastasis by re-annotating microarray data from the Gene Expression Omnibus (GEO) microarray dataset. These findings reveal potential roles for lncRNAs during melanoma tumorigenesis and metastasis and provide a rich candidate reservoir for future studies.

Published
2016

2. Identification and Characterization of a Novel Non-Coding RNA Involved in Sperm Maturation

Author

Yonglian Zhang, Li Zhang, Min-hua Lu, Qiang Liu, Mo-Fang Liu, Jinsong Zhang, Minjie Ni, and Zhi-Hong Hu

Subjects
Male, Small RNA, RNA, Untranslated, Time Factors, lcsh:Medicine, Biochemistry, MiRBase, Rats, Sprague-Dawley, Molecular cell biology, Gene expression, RNA Precursors, Cloning, Molecular, Small nucleolar RNA, lcsh:Science, Epididymis, Genetics, Multidisciplinary, biology, Chromosome Biology, Physics, Animal Models, Non-coding RNA, Chromatin, Nucleic acids, Organ Specificity, Cellular Types, Research Article, Molecular Sequence Data, Biophysics, Down-Regulation, Real-Time Polymerase Chain Reaction, Model Organisms, microRNA, Animals, RNA, Messenger, Biology, Inflammation, Messenger RNA, Base Sequence, Sequence Analysis, RNA, lcsh:R, RNA stability, Clone Cells, Rats, Sperm Maturation, Germ Cells, RNA processing, Gene Expression Regulation, biology.protein, RNA, Rat, lcsh:Q, Sperm Capacitation, Dicer
Abstract

A long and ever-expanding roster of small (∼20-30 nucleotides) RNAs has emerged during the last decade, and most can be subsumed under the three main headings of microRNAs (miRNAs), Piwi-interacting RNAs (piRNAs), and short interfering RNAs (siRNAs). Among the three categories, miRNAs is the most quickly expanded group. The most recent number of identified miRNAs is 16,772 (Sanger miRbase, April 2011). However, there are insufficient publications on their primary forms, and no tissue-specific small RNAs precursors have been reported in the epididymis. Here, we report the identification in rats of an epididymis-specific, chimeric, noncoding RNA that is spliced from two different chromosomes (chromosomes 5 and 19), which we named HongrES2. HongrES2 is a 1.6 kb mRNA-like precursor that gives rise to a new microRNA-like small RNA (mil-HongrES2) in rat epididymis. The generation of mil-HongrES2 is stimulated during epididymitis. An epididymis-specific carboxylesterase named CES7 had 100% cDNA sequence homology at the 3'end with HongrES2 and its protein product could be downregulated by HongrES2 via mil-HongrES2. This was confirmed in vivo by initiating mil-HongrES2 over-expression in rats and observing an effect on sperm capacitation.

Published
2011

3. Rehmannia Glutinosa Extract Activates Endothelial Progenitor Cells in a Rat Model of Myocardial Infarction through a SDF-1 α/CXCR4 Cascade.

Author

Ying-Bin Wang, Yun-Fang Liu, Xiao-Ting Lu, Fang-Fang Yan, Bo Wang, Wen-Wu Bai, and Yu-Xia Zhao

Subjects
*ENDOTHELIAL cells, *WESTERN immunoblotting, *CHINESE medicine, *ISCHEMIA, *BONE marrow, *MESSENGER RNA
Abstract

Objectives: Endothelial progenitor cells (EPCs) can be used to repair tissues after myocardial infarction (MI) but EPC activators have adverse reactions. Rehmannia glutinosa is a herb used in traditional Chinese medicine, which can promote bone-marrow proliferation and protect the ischemic myocardium. We investigated the effects of Rehmannia glutinosa extract (RGE) on EPCs in a rat model of MI. Methods: A total of 120 male Wistar rats were randomized to 2 groups (n = 60 each) for treatment: high-dose RGE (1.5 g⋅kg-1⋅day-1 orally) for 8 weeks, then left anterior descending coronary artery ligation, mock surgery or no treatment, then RGE orally for 4 weeks; or normal saline (NS) as the above protocol. The infarct region of the left ventricle was assessed by serial sectioning and morphology. EPCs were evaluated by number and function. Protein and mRNA levels of CD133, vascular endothelial growth factor receptor 2 (VEGFR2), chemokine C-X-C motif receptor 4 (CXCR4), stromal cell-derived factor-1α (SDF-1α) were measured by immunohistochemistry, Western blot and quantitative PCR analysis. Results:RGE significantly improved left ventricular function, decreased the ischemic area and the apoptotic index in the infarct myocardium, also decreased the concentration of serum cardiac troponin T and brain natriuretic peptide at the chronic stage after MI (from week 2 to week 4). RGE increased EPC number, proliferation, migration and tube-formation capacity. It was able to up-regulate the expression of angiogenesis-associated ligand/receptor, including CD133, VEGFR2 and SDF-1α/CXCR4. In vitro, the effect of RGE on SDF-1a/CXCR4 cascade was reversed by the CXCR4 specific antagonist AMD3100. Conclusion: RGE may enhance the mobilization, migration and therapeutic angiogenesis of EPCs after MI by activating the SDF-1α/CXCR4 cascade. [ABSTRACT FROM AUTHOR]

Published
2013
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4. Icaritin Shows Potent Anti-Leukemia Activity on Chronic Myeloid Leukemia In Vitro and In Vivo by Regulating MAPK/ERK/JNK and JAK2/STAT3 /AKT Signalings.

Author

Jian feng Zhu, Zi jian Li, Guang sen Zhang, Kun Meng, Wen yong Kuang, Jin Li, Xin fu Zhou, Rui juan Li, Hong ling Peng, Chong wen Dai, Jian Kai Shen, Fan jie Gong, Yun xiao Xu, and Su fang Liu

Subjects
CHRONIC myeloid leukemia, CELLULAR signal transduction, APOPTOSIS, MICE, WESTERN immunoblotting, BONE marrow, MESSENGER RNA, CELL proliferation
Abstract

Purpose: To explore the effects of Icaritin on chronic myeloid leukemia (CML) cells and underlying mechanisms. Method: CML cells were incubated with various concentration of Icaritin for 48 hours, the cell proliferation was analyzed by MTT and the apoptosis was assessed with Annexin V and Hoechst 33258 staining. Cell hemoglobinization was determined. Western blotting was used to evaluate the expressions of MAPK/ERK/JNK signal pathway and Jak-2/Phorpho-Stat3/Phorsph- Akt network-related protein. NOD-SCID nude mice were applied to demonstrate the anti-leukemia effect of Icaritin in vivo. Results: Icaritin potently inhibited proliferation of K562 cells (IC50 was 8 &mgr;M) and primary CML cells (IC50 was 13.4 &mgr;M for CML-CP and 18 &mgr;M for CML-BC), induced CML cells apoptosis and promoted the erythroid differentiation of K562 cells with time-dependent manner. Furthermore, Icaritin was able to suppress the growth of primary CD34+ leukemia cells (CML) and Imatinib-resistant cells, and to induce apoptosis. In mouse leukemia model, Icaritin could prolong lifespan of NOD-SCID nude mice inoculated with K562 cells as effective as Imatinib without suppression of bone marrow. Icaritin could upregulate phospho-JNK or phospho-C-Jun and down-regulate phospho-ERK, phospho-P-38, Jak-2, phospho-Stat3 and phospho-Akt expression with dose- or time-dependent manner. Icaritin had no influence both on c-Abl and phospho-c-Abl protein expression and mRNA levels of Bcr/Abl. [ABSTRACT FROM AUTHOR]

Published
2011
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