51. Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation.
- Author
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Hatanaka, Elaine, Dermargos, Alexandre, Hirata, Aparecida Emiko, Vinolo, Marco Aurélio Ramirez, Carpinelli, Angelo Rafael, Newsholme, Philip, Armelin, Hugo Aguirre, and Curi, Rui
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FIBROBLASTS , *OLEIC acid , *LINOLEIC acid , *LINOLENIC acids , *NADPH oxidase , *CHEMILUMINESCENCE , *MESSENGER RNA , *GENE expression - Abstract
The effect of oleic, linoleic and γ-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic and γ-linolenic acids. ROS production was dependent on the addition of β-NADH or NADPH to the medium. Diphenyleneiodonium inhibited the effect of oleic, linoleic and γ-linolenic acids on fibroblast superoxide release by 79%, 92% and 82%, respectively. Increased levels of p47phox phosphorylation due to fatty acid treatment were detected by Western blotting analyses of fibroblast proteins. Increased p47phox mRNA expression was observed using real-time PCR. The rank order for the fatty acid stimulation of the fibroblast oxidative burst was as follows: γ-linolenic > linoleic > oleic. In conclusion, oleic, linoleic and γ-linolenic acids stimulated ROS production via activation of the NADPH oxidase enzyme complex in fibroblasts. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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