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Predictive models are often complex to produce and interpret, yet can offer valuable insights for management, conservation and policy-making. Here we introduce a new modelling tool (the R package 'BBNet'), which is simple to use, and requires little mathematical or computer programming background. By using straightforward concepts to describe interactions between model components, predictive models can be effectively constructed using basic spreadsheet tools and loaded into the R package. These models can be analysed, visualised, and sensitivity tested to assess how information flows through the system's components and provide predictions for future outcomes of the systems. This paper provides a theoretical background to the models, which are modified Bayesian belief networks (BBNs), and an overview of how the package can be used. The models are not fully quantitative, but outcomes between different modelled scenarios can be considered ordinally (i.e. ranked from 'best' to 'worse'). Parameterisation of models can also be through data, literature, expert opinion, questionnaires and/or surveys of opinion, which are expressed as a simple 'weak' to 'very strong' or 1–4 integer value for interactions between model components. While we have focussed on the use of the models in environmental and ecological problems (including with links to management and social outcomes), their application does not need to be restricted to these disciplines, and use in financial systems, molecular biology, political sciences and many other disciplines are possible. [ABSTRACT FROM AUTHOR]

Aims: Activation of Protease Activated Receptor 2 (PAR2) has been shown to be involved in regulation of injury-related processes including inflammation, fibrosis and hypertrophy. In this study we will investigate the role of PAR2 in cardiac injury in a mouse model of hypertension using continuous infusion with angiotensin II. Methods: Hypertension was induced in 12 weeks old wildtype (wt, n = 8) and PAR2 deficient mice (n = 9) by continuous infusion with angiotensin II for 4 weeks using osmotic minipumps. At the end, hearts were collected for analysis of left ventricular hypertrophy (LVH), myocardial capillary supply, fibrosis and localization of PAR2 expression using histological, immunohistological and mRNA expression analysis techniques. In addition, rat cardiac fibroblasts were treated with angiotensin II and PAR2 was inhibited by a blocking antibody and the PAR2 inhibitor AZ3451. Results: Cardiac PAR2 mRNA expression was downregulated by 40±20% in wt mice treated with AngII compared to untreated controls. Four weeks after AngII treatment, LVH was significantly increased in AngII-treated wt mice compared to similarly treated PAR2-deficient animals as determined by relative heart weight, left ventricular cross-sectional area, and analysis of ventricular lumen area determined on sections. Treatment of wt mice resulted in an approximately 3-fold increase in cardiac expression of FGF23, which was 50% lower in PAR2-deficient animals compared to wt animals and therefore no longer significantly different from expression levels in untreated control mice. In contrast, cardiac interstitial fibrosis was significantly higher in PAR2-deficient mice compared to similar treated wt controls, as assessed by Sirius Red staining (>3-fold) and collagen IV staining (>2-fold). Additional experiments with isolated cardiac fibroblasts showed induction of pro-fibrotic genes when treated with PAR2 inhibitors. Conclusion: In angiotensin II-induced cardiac injury, PAR2 deficiency has an ambivalent effect, enhancing fibrosis on the one hand, but reducing LVH on the other. [ABSTRACT FROM AUTHOR]

Early literacy skills, the foundational abilities necessary for the development of literacy, must be examined holistically in preschool children. This study aimed to investigate early literacy skills in preschool children and determine how variables related to literacy development can be clustered by using a dendrogram. The study was conducted on 166 preschool children (75 female, 91 male; mean age: 65.9±4.4 months). Hierarchical cluster analysis (CA) was used to find the variable clustering trends. The 44 variables related to literacy (sociodemographic factors related to the child and family, child–parent relationship, child's behavior, and social, sensory integration, motor, and auditory linguistic skills) that were closest to each other in the dendrogram were clustered, and the situation was summarized by reducing them to two main clusters and six sub-clusters. This study elaborates on the implications of reducing multivariate complexity using CA. It is recommended that the relationships among the variables in this dendrogram structure are considered when new hypotheses and studies related to early literacy are designed. [ABSTRACT FROM AUTHOR]

Pesticides are chemicals used to enhance crop production. However, their residues can persist and accumulate in various environmental settings. This study assessed the occurrence of pesticide residues and ecological risks in surface water and sediment from the Libga and Builpela dams in northern Ghana. A total of 42 pesticides were analysed. Toxic units were used to assess the acute risk to sediment-dwelling organisms and aquatic biota. Risk quotients were employed to assess the chronic risk to aquatic organisms. Chlorpyrifos, atrazine, profenofos, and chlorfenvinphos were the main chemicals found in surface water. The concentrations were highest near the Builpela dam (0.413μg/L, 2.927μg/L, 0.304μg/L, 0.692μg/L, 0.073μg/L, 0.290μg/L, 0.06μg/L, and absent in the Libga dam). In the sediment, the organophosphates pyrimiphos-methyl and chlorpyrifos were found at high concentrations. They were found in higher quantities in the Libga dam (0.554mg/kg and 0.052mg/kg, respectively) and Builpela dam (0.051mg/kg and 0.043mg/kg, respectively). For organochlorines, p,p'-DDE and p,p'-DDD were the main residues detected at high concentrations. However, concentrations were higher for p,p'-DDD at Builpela than for p,p'-DDE. Additionally, high concentrations of atrazine were detected in this study. The toxic units showed a high acute risk for organisms that live in sediment as a result of chlorpyrifosfos and chlorfenvinphos residues. Similarly, pirimiphos-methyl and chlorfenvinphos, followed by chlorpyrifos, contributed to high acute risk in aquatic invertebrates. Risk quotients showed that both dams had a high long-term risk for aquatic life; however, the risk was higher at the Builpela dam due to Pirimiphos-methyl and Chlorfenvinphos. Ghana's pesticide regulations are less comprehensive and enforcement is often weaker in protecting aquatic organisms. It is recommended to enforce strict limits on pesticide residues. Additionally, there is a need to regularly review and update these regulations based on new scientific data to protect aquatic ecosystems. [ABSTRACT FROM AUTHOR]

Background: Children have alarmingly low levels of competency in fundamental motor skills (FMS) and high levels of physical inactivity. e:health interventions, interventions delivered electronically, are useful tools for intervention in the home through parents, but less is known about the effects of these interventions in early childhood education centers or settings. Therefore, we created the Motor skills At Playtime (MAP) e:health intervention (e:MAP) to be delivered in an early childhood education setting. The goals of this pilot study on e:MAP are to (1) determine the intervention effects on children's FMS and physical activity and (2) explore the teachers' perceptions and ability to facilitate e:MAP. Methods: This pilot study uses a pretest/posttest randomized cluster control design. We will recruit at least 64 children (3.5–5 years of age) enrolled in a single early childhood education center. Children will be randomly assigned at the level of the classroom to an e:MAP group (n~30) or a control group (n~30). Children in classrooms assigned to e:MAP will complete an 8-week intervention. We will collect measures of child FMS and physical activity, and teacher's perceptions of the program before (pretest) and after the intervention (posttest). FMS measures include process (Test of Gross Motor Development-3rd Edition) and product-oriented scores. Physical activity will be assessed using a 7-day accelerometer wear protocol. Teachers' perceptions will be assessed through a brief survey. Lastly, we will collect data on teachers' ability to facilitate e:MAP through a daily survey. Potential significance: This study will yield novel insights into the effectiveness and feasibility of a health intervention in an early childhood education setting. Results from this work will expand our knowledge of how to harness e:health modalities, which have the potential to significantly expand the distribution and scalability of FMS interventions. [ABSTRACT FROM AUTHOR]

The label-feedback hypothesis states that language can modulate visual processing. In particular, hearing or reading aloud target names (labels) speeds up performance in visual search tasks by facilitating target detection and such advantage is often measured against a condition where the target name is shown visually (i.e. via the same modality as the search task). The current study conceptually complements and expands previous investigations. The effect of a multimodal label presentation (i.e., an audio+visual, AV, priming label) in a visual search task is compared to that of a multimodal (i.e. white noise+visual, NV, label) and two unimodal (i.e. audio, A, label or visual, V, label) control conditions. The name of a category (i.e. a label at the superordinate level) is used as a cue, instead of the more commonly used target name (a basic level label), with targets belonging to one of three categories: garments, improper weapons, and proper weapons. These categories vary for their structure, improper weapons being an ad hoc category (i.e. context-dependent), unlike proper weapons and garments. The preregistered analysis shows an overall facilitation of visual search performance in the AV condition compared to the NV condition, confirming that the label-feedback effect may not be explained away by the effects of multimodal stimulation only and that it extends to superordinate labels. Moreover, exploratory analyses show that such facilitation is driven by the garments and proper weapons categories, rather than improper weapons. Thus, the superordinate label-feedback effect is modulated by the structural properties of a category. These findings are consistent with the idea that the AV condition prompts an "up-regulation" of the label, a requirement for enhancing the label's beneficial effects, but not when the label refers to an ad hoc category. They also highlight the peculiar status of the category of improper weapons and set it apart from that of proper weapons. [ABSTRACT FROM AUTHOR]

Working memory (WM) and inhibitory control (IC) play a crucial role in learning during early childhood. The literature suggests a non-linear developmental trajectory of executive functions (EFs) with varied results according to gender, usually attributed to environmental factors. However, there is insufficient and inconclusive data on whether this pattern is reproduced in the Latin American preschool population since most studies have been conducted in English-speaking, European, and Asian environments. Thus, objectively comparing children's executive performance across diverse international geographical contexts becomes challenging. This study aimed to conduct a cross-sectional analysis of the performance in WM and IC of 982 Ecuadorian preschoolers aged between 42 and 65 months (M = 53.71; SD = 5.714) and belonging to medium-high, medium, and low-medium socioeconomic strata. The participants consisted of 496 boys (M = 53.77; SD = 5.598) and 486 girls (M = 53.65; SD = 5.834), representing nine cities in Ecuador. To assess the effect of age and gender on performance in these two domains, the sample was divided into four 6-month age intervals. Two tests were administered to the participants, and a survey was conducted with 799 of their usual caregivers. Viewing the cross-sectional mean scores of the WM and IC tests as a temporal continuum reveals an upward trend in each age interval studied. Girls outperformed boys on the IC test, showing statistically significant differences in the earliest age interval. The gender differences in executive performance reported in the literature emphasize the need to explore the modulating effect of environmental variables on early childhood development. This information could offer valuable insights for adapting and optimizing cognitive and didactic strategies in early childhood tailored to the characteristics and needs of the preschool population. [ABSTRACT FROM AUTHOR]

Aquatic invertebrates provide important ecosystem services, including decomposition and nutrient cycling, and provide nutrition for birds, fish, amphibians, and bats. Thus, the effects of agricultural land management practices on aquatic invertebrates are relevant to farmers, wildlife biologists, and policymakers. Here, we used data on aquatic invertebrates (159 taxa, 73 to species, 75 to genus/family) collected in 40 wetlands in the Canadian prairies to test for direct and indirect relationships among land management types (perennial cover, organic, minimum tillage, conventional), landscape structure (cropland and wetland cover within the surrounding landscape), and water quality (total nutrient levels, turbidity) on species richness of invertebrates using structural equation modelling. Additionally, we assessed variation in community composition within and among wetlands in different land use management types using a direct gradient analysis and variance partitioning. The direct effects of land management type were not supported but we found strong supportive evidence that effects of land management on richness were significantly mediated through cropland cover, nutrient levels, and turbidity. After controlling for these indirect effects, aquatic invertebrate richness decreased along a gradient from the lowest to the highest farming intensity, i.e., richness decreased from perennial cover sites to organic to minimum tillage to conventional sites. Support was also found for negative effects of nutrient levels and turbidity on richness. We did not find significant support for differences in gamma diversity or a simple test (homogeneity of multivariate dispersions) of differences in turnover among land management types; however, land management had a significant effect in distance-based redundancy analysis. Taken together, these results suggest that focusing conservation efforts on reducing cropland erosion and nutrient inputs to wetlands and creating more permanent cover may be effective strategies for conserving richness of aquatic invertebrates in agricultural landscapes in this region. [ABSTRACT FROM AUTHOR]

The present contribution aimed to analyze the effects of a motor program intervention (i.e., I-MovE intervention) implemented indoors and outdoors at nursery school, on children's motor, socio-emotional, and cognitive skills. The study uses a non-randomized pre-post test design. Participants were children attending twenty nursery schools in the North of Italy. The intervention activities were adapted to age: Level 1 activities were addressed to children between 6 and 12 months, and Level 2 activities were addressed to children between 13 and 43 months. Within each level, one group of children developed the intervention indoors (IN-group; Level 1: n = 10; Level 2: n = 104) and another group developed the intervention outdoors (OUT-Group; Level 1: n = 12; Level 2: n = 66). Finally, one additional group was involved as the control group (CONT-Group; Level 1: n = 15; Level 2: n = 98). Children's motor, cognitive, and socio-emotional skills were assessed before and after the intervention by nursery school teachers. The main results showed that the motor intervention promoted children's motor skills development in both groups (i.e., groups implementing Levels 1 and 2 activities) and the cognitive and socio-emotional skills in the older group (i.e., group implementing Level 2 activities), especially the group that performed the intervention outdoors. [ABSTRACT FROM AUTHOR]

Introduction: Increased plasma trimethylamine oxide (TMAO) is observed in cardiovascular and metabolic diseases, originating from the gut microbiota product, trimethylamine (TMA), via flavin-containing monooxygenases (FMOs)-dependent oxidation. Numerous studies have investigated the association between plasma TMAO and various pathologies, yet limited knowledge exists regarding tissue concentrations of TMAO, TMAO precursors, and interspecies variability. Methods: Chromatography coupled with mass spectrometry was employed to evaluate tissue concentrations of TMAO and its precursors in adult male mice, rats, and guinea pigs. FMO mRNA and protein levels were assessed through PCR and Western blot, respectively. Results: Plasma TMAO levels were similar among the studied species. However, significant differences in tissue concentrations of TMAO were observed between mice, rats, and guinea pigs. The rat renal medulla exhibited the highest TMAO concentration, while the lowest was found in the mouse liver. Mice demonstrated significantly higher plasma TMA concentrations compared to rats and guinea pigs, with the highest TMA concentration found in the mouse renal medulla and the lowest in the rat lungs. FMO5 exhibited the highest expression in mouse liver, while FMO3 was highly expressed in rats. Guinea pigs displayed low expression of FMOs in this tissue. Conclusion: Despite similar plasma TMAO levels, mice, rats, and guinea pigs exhibited significant differences in tissue concentrations of TMA, TMAO, and FMO expression. These interspecies variations should be considered in the design and interpretation of experimental studies. Furthermore, these findings may suggest a diverse importance of the TMAO pathway in the physiology of the evaluated species. [ABSTRACT FROM AUTHOR]

Background: Chronic hepatitis B (CHB) is endemic in the Aboriginal and Torres Strait Islander population of Australia's Northern Territory. Progression to liver disease can be prevented if holistic care is provided. Low health literacy amongst health professionals is a known barrier to caring for people living with CHB. We co-designed and delivered a culturally safe "Managing hepatitis B" training course for the Aboriginal health workforce. Here, we present an evaluation of the course. Objectives: 1. To improve course participants CHB-related knowledge, attitudes, and clinical practice. 2. To evaluate the "Managing hepatitis B" training course. 3. To enable participants to have the skills and confidence to be part of the care team. Methods: We used participatory action research and culturally safe principles. We used purpose-built quantitative and qualitative evaluation tools to evaluate our "Managing hepatitis B" training course. We integrated the two forms of data, deductively analysing codes, grouped into categories, and assessed pedagogical outcomes against Kirkpatrick's training evaluation framework. Results: Eight courses were delivered between 2019 and 2023, with 130 participants from 32 communities. Pre- and post-course questionnaires demonstrated statistically significant improvements in all domains, p<0.001 on 93 matched pairs. Thematic network analysis demonstrated high levels of course acceptability and significant knowledge acquisition. Other themes identified include cultural safety, shame, previous misinformation, and misconceptions about transmission. Observations demonstrate improvements in post-course engagement, a deep understanding of CHB as well as increased participation in clinical care teams. Conclusions: The "Managing hepatitis B" training course led to a sustained improvement in the knowledge and attitudes of the Aboriginal health workforce, resulting in improved care and treatment uptake for people living with CHB. Important non-clinical outcomes included strengthening teaching and leadership skills, and empowerment. [ABSTRACT FROM AUTHOR]

The fairness of decisions made at various stages of the publication process is an important topic in meta-research. Here, based on an analysis of data on the gender of authors, editors and reviewers for 23,876 initial submissions and 7,192 full submissions to the journal eLife, we report on five stages of the publication process. We find that the board of reviewing editors (BRE) is men-dominant (69%) and that authors disproportionately suggest male editors when making an initial submission. We do not find evidence for gender bias when Senior Editors consult Reviewing Editors about initial submissions, but women Reviewing Editors are less engaged in discussions about these submissions than expected by their proportion. We find evidence of gender homophily when Senior Editors assign full submissions to Reviewing Editors (i.e., men are more likely to assign full submissions to other men (77% compared to the base assignment rate to men RE of 70%), and likewise for women (41% compared to women RE base assignment rate of 30%))). This tendency was stronger in more gender-balanced scientific disciplines. However, we do not find evidence for gender bias when authors appeal decisions made by editors to reject submissions. Together, our findings confirm that gender disparities exist along the editorial process and suggest that merely increasing the proportion of women might not be sufficient to eliminate this bias. Measures accounting for women's circumstances and needs (e.g., delaying discussions until all RE are engaged) and raising editorial awareness to women's needs may be essential to increasing gender equity and enhancing academic publication. [ABSTRACT FROM AUTHOR]

Aim of the study: Estimate the magnitude and factors associated with risk factors for chronic noncommunicable diseases in adolescents and young adults in Brazil. Methods: Cross-sectional study that analyzed data from the 2019 National Health Survey. The population of interest was adolescents and young adults aged 15 to 24 years. Data were collected through individual interviews during home visits. Dependent variables included major risk factors for chronic noncommunicable diseases. Demographic and socioeconomic characteristics were used as independent variables. Multiple Poisson regression models were used to assess the relationship between independent variables and risk factors. Results: A total of 10,460 individuals (5,001 men and 5,459 women) were included. Regardless of sex, the most prevalent risk factors were insufficient fruit and vegetable consumption (92.6%) and leisure-time physical inactivity (43.3%). The prevalence rates of tobacco smokers, alcohol consumption once a month or more, and alcohol abuse were 8.9%, 28.7%, and 18.5%, respectively. Regular consumption of soft drinks and/or artificial juices was described by 17.2%. The prevalence of overweight was 32.5%. Young adults, males, and individuals with lower educational levels, of black race/skin color, with lower household income, and residents of urban areas had a higher prevalence for most risk factors. Differences in the determinants were found for some factors. Inequalities between Brazilian regions were recorded for seven of the nine factors analyzed. The most socioeconomically developed regions had the highest prevalence of most risk factors. The high magnitude of risk factors indicates a potential increase in the burden of chronic noncommunicable diseases in a future scenario for Brazil. [ABSTRACT FROM AUTHOR]

The existence of handle affordances has been classically demonstrated using the Stimulus-Response Compatibility paradigm, with shorter response times when the orientation of the object handle and the response hand are compatible in comparison to incompatible. Yet the activation of handle affordances from visual objects has been investigated in very simple situations involving single stimulus and motor response. As natural perceptual scenes are usually composed of multiple objects that could activate multiple affordances, the consequence of multiple affordance activation on the perception and processing of a given object of the scene requires more investigation. The aim of this study was to determine the impact of distractor affordances on the processing of a target object in situations involving several familiar graspable objects. In two online experiments, 229 participants had to select a target object (the kitchen utensil or the tool) in a visual scene displaying a pair of objects. They performed left key presses when the target was on the left and right key presses when the target was on the right. Target handle orientation and response side could be compatible or incompatible. Critically, target and distractor objects had similar or dissimilar handle affordances, with handles oriented for left- or right-hand grasps. Results from the two experiments showed slower response times when target and distractor objects had similar handle affordances in comparison to dissimilar affordances, when participants performed right hand responses and when target orientation and response were compatible. Thus, affordance similarity between objects may interfere rather than facilitate object processing and slow down target selection. These findings are in line with models of affordance and object selection assuming automatic inhibition of distractors' affordances for appropriate object interaction. [ABSTRACT FROM AUTHOR]

The study of optimal best practice, coinciding with a person's 'motivational mindset', is an interesting research inquiry for development. Optimal best practice, in brief, relates to the maximization of a person's state of functioning (e.g., cognitive functioning). Moreover, the nature of optimal best practice is positive and motivational, helping individuals to flourish in different courses of action (e.g., academic performance at school). Several research undertakings, non-experimental in design, have provided clear and consistent evidence to substantiate the existing viewpoints and perspectives of optimal best practice. Our proposed investigation, which involved physical education pre-service teacher students from Spain (N = 681), explored one notable focus of inquiry–namely, the formation of optimal best practice and its predictive and explanatory account on future adaptive outcomes. As such, using Likert-scale measures and path analysis techniques, we were able to identify two associative patterns: achievement of optimal best practice is positively accounted for by academic self-concept, optimism, and current best practice and, in contrast, negatively accounted for by pessimism; and that optimal best practice could act as a determinant of academic engagement for effective learning. Such associations are significant, providing relevant information for different teaching and research purposes. [ABSTRACT FROM AUTHOR]

In recent years, couples have been affected by health measures related to COVID-19, a circumstance that forces us to examine couple interactions in terms of crucial variables of their functioning. In this sense, the present study aimed to examine the association between love, jealousy, satisfaction, and violence in young couples through network analysis. A total of 834 young people and adults between 18 and 38 years of age (Mean = 20.97, SD = 2.39) participated; 646 women (77.50%) and 188 men (22.50%), who completed the Sternberg's love scale (STLS-R), Brief Jealousy Scale (BJS), Relationship Assessment Scale (RAS) and Woman Abuse Screening Tool (WAST-2). A partial unregularized network was estimated using the ggmModSelect function. The Bridge Strength index was calculated because the aim was to identify the bridge nodes between the variables under study. The results reveal that two nodes of the love variable, Commitment, and Intimacy, had a direct and moderate relationship with the Satisfaction node. The latter is the central node in the network. However, in the male group, the most intense associations are in Satisfaction-Intimacy, Violence-Passion, Jealousy-Commitment. It is concluded that there are relevant connections between the nodes of the network, which invite further research on couple relationships after the COVID-19 pandemic. [ABSTRACT FROM AUTHOR]

Introduction: Self-regulation (SR) is critical to healthy development in children, and intervention approaches (i.e., professional training, classroom-based curricula, parent-focused intervention) have shown to support or enhance SR. However, to our knowledge, none have tested whether changes in children's SR across an intervention relate to changes in children's health behavior and outcomes. This study, the Promoting Activity and Trajectories of Health (PATH) for Children-SR Study uses a cluster-randomized control trial to examine the immediate effects of a mastery-climate motor skills intervention on SR. Secondly, this study examines the associations between changes in SR and changes in children's health behaviors (i.e., motor competence, physical activity, and perceived competence) and outcomes (i.e., body mass index and waist circumference) (ClinicalTrials.gov Identifier, NCT03189862). Methods and analysis: The PATH—SR study will be a cluster-randomized clinical trial. A total of 120 children between the ages of 3.5 to 5 years of age will be randomized to a mastery-climate motor skills intervention (n = 70) or control (n = 50) condition. SR will be assessed using measures that evaluate cognitive SR (cognitive flexibility and working memory), behavioral SR (behavioral inhibition), and emotional SR (emotional regulation). Health behaviors will be assessed with motor skills, physical activity, and perceived competence (motor and physical) and health outcomes will be waist circumference and body mass index. SR, health behaviors, and health outcomes will be assessed before and after the intervention (pre-test and post-test). Given the randomization design, 70 children in the intervention group and 50 in the control group, we have 80% power to detect an effect size of 0.52, at a Type I error level of 0.05. With the data collected, we will test the intervention effect on SR with a two-sample t-test comparing the intervention group and the control group. We will further evaluate the associations between changes in SR and changes in children's health behaviors and health outcomes, using mixed effect regression models, with a random effect to account for within-subject correlations. The PATH-SR study addresses gaps in pediatric exercise science and child development research. Findings hold the potential to help shape public health and educational policies and interventions that support healthy development during the early years. Ethics and dissemination: Ethical approval for this study was obtained through the Health Sciences and Behavioral Sciences Institutional Review Board, University of Michigan (HUM00133319). The PATH-SR study is funded by the National Institutes of Health Common Fund. Findings will be disseminated via print, online media, dissemination events and practitioner and/or research journals. Trial registration number: ClinicalTrials.gov Identifier, NCT03189862. [ABSTRACT FROM AUTHOR]

Recent advances in human induced pluripotent stem cell (hiPSC)-derived cardiac microtissues provide a unique opportunity for cardiotoxic assessment of pharmaceutical and environmental compounds. Here, we developed a series of automated data processing algorithms to assess changes in action potential (AP) properties for cardiotoxicity testing in 3D engineered cardiac microtissues generated from hiPSC-derived cardiomyocytes (hiPSC-CMs). Purified hiPSC-CMs were mixed with 5–25% human cardiac fibroblasts (hCFs) under scaffold-free conditions and allowed to self-assemble into 3D spherical microtissues in 35-microwell agarose gels. Optical mapping was performed to quantify electrophysiological changes. To increase throughput, AP traces from 4x4 cardiac microtissues were simultaneously acquired with a voltage sensitive dye and a CMOS camera. Individual microtissues showing APs were identified using automated thresholding after Fourier transforming traces. An asymmetric least squares method was used to correct non-uniform background and baseline drift, and the fluorescence was normalized (ΔF/F0). Bilateral filtering was applied to preserve the sharpness of the AP upstroke. AP shape changes under selective ion channel block were characterized using AP metrics including stimulation delay, rise time of AP upstroke, APD30, APD50, APD80, APDmxr (maximum rate change of repolarization), and AP triangulation (APDtri = APDmxr−APD50). We also characterized changes in AP metrics under various ion channel block conditions with multi-class logistic regression and feature extraction using principal component analysis of human AP computer simulations. Simulation results were validated experimentally with selective pharmacological ion channel blockers. In conclusion, this simple and robust automated data analysis pipeline for evaluating key AP metrics provides an excellent in vitro cardiotoxicity testing platform for a wide range of environmental and pharmaceutical compounds. [ABSTRACT FROM AUTHOR]

Recent work has shown potential in using Mixed Integer Programming (MIP) solvers to optimize certain aspects of neural networks (NNs). However the intriguing approach of training NNs with MIP solvers is under-explored. State-of-the-art-methods to train NNs are typically gradient-based and require significant data, computation on GPUs, and extensive hyper-parameter tuning. In contrast, training with MIP solvers does not require GPUs or heavy hyper-parameter tuning, but currently cannot handle anything but small amounts of data. This article builds on recent advances that train binarized NNs using MIP solvers. We go beyond current work by formulating new MIP models which improve training efficiency and which can train the important class of integer-valued neural networks (INNs). We provide two novel methods to further the potential significance of using MIP to train NNs. The first method optimizes the number of neurons in the NN while training. This reduces the need for deciding on network architecture before training. The second method addresses the amount of training data which MIP can feasibly handle: we provide a batch training method that dramatically increases the amount of data that MIP solvers can use to train. We thus provide a promising step towards using much more data than before when training NNs using MIP models. Experimental results on two real-world data-limited datasets demonstrate that our approach strongly outperforms the previous state of the art in training NN with MIP, in terms of accuracy, training time and amount of data. Our methodology is proficient at training NNs when minimal training data is available, and at training with minimal memory requirements—which is potentially valuable for deploying to low-memory devices. [ABSTRACT FROM AUTHOR]

Biophysical models are a powerful tool for assessing population connectivity of marine organisms that broadcast spawn. Albula vulpes is a species of bonefish that is an economically and culturally important sportfish found throughout the Caribbean and that exhibits genetic connectivity among geographically distant populations. We created ontogenetically relevant biophysical models for bonefish larval dispersal based upon multiple observed spawning events in Abaco, The Bahamas in 2013, 2018, and 2019. Biological parameterizations were informed through active acoustic telemetry, CTD casts, captive larval rearing, and field collections of related albulids and anguillids. Ocean conditions were derived from the Regional Navy Coastal Ocean Model American Seas dataset. Each spawning event was simulated 100 times using the program Ichthyop. Ten-thousand particles were released at observed and putative spawning locations and were allowed to disperse for the full 71-day pelagic larval duration for A. vulpes. Settlement densities in defined settlement zones were assessed along with interactions with oceanographic features. The prevailing Northern dispersal paradigm exhibited strong connectivity with Grand Bahama, the Berry Islands, Andros, and self-recruitment to lower and upper Abaco. Ephemeral gyres and flow direction within Northwest and Northeast Providence Channels were shown to have important roles in larval retention to the Bahamian Archipelago. Larval development environments for larvae settling upon different islands showed few differences and dispersal was closely associated with the thermocline. Settlement patterns informed the suggestion for expansion of conservation parks in Grand Bahama, Abaco, and Andros, and the creation of a parks in Eleuthera and the Berry Islands to protect fisheries. Further observation of spawning events and the creation of biophysical models will help to maximize protection for bonefish spawning locations and nursery habitat, and may help to predict year-class strength for bonefish stocks throughout the Greater Caribbean. [ABSTRACT FROM AUTHOR]

In a digital and knowledge based economy, intangible assets are predominant and their role along with age and knowledge has become key success factors for firms. However, a very little attention was given to the intangible assets in the banking sectors' in Ethiopia and the effect still not studied yet. Therefore, the aim of this study is to empirically examine the effect of intangible assets on the financial performance and policy of 17 commercial banks in Ethiopia from the year 2017 to 2020. Return on asset and equity were used to measure the financial performance and debt as a measure of financial policy. The intangible asset is used as the main explanatory variable and asset size and liquidity as control variables. Random effect estimation technique for panel data was used. The result revealed that intangible asset has positive effect on the financial performance measured both by ROA and ROE at 5% significance level while, negative effect on the financial policy of commercial banks in Ethiopia at 1% significance level. Moreover, the study found asset size has significant and positive effect on ROA and ROE at 1% and 5% significance level respectively. Liquidity ratio has also significant positive effect on the financial performance measured both by ROA and ROE at 5% significance level. Finally, the finding revealed asset size and liquidity ratio has significant positive effect on the financial policy of commercial banks in Ethiopia at 10% and 1% significance level respectively. Therefore, the study concludes that financial performance and policy is achieved not only by using physical assets but also using intangible assets. Thus, the boards and mangers of commercial banks' ought to plan and maintain the appropriate ratio of intangible assets to total assets for securing sustainable development in achieving the maximization of shareholders wealth and to have optimum debt. [ABSTRACT FROM AUTHOR]

Objectives: We aimed to assess women's perceptions on the long-term risks for cardiovascular disease (CVD) after major pregnancy complications. Methods: Women who experienced major pregnancy complications and those who experienced uncomplicated pregnancies were invited to participate in a qualitative study. Focus group discussions (FGDs) and self-administered questionnaires were used to explore: The knowledge of long-term sequelae after experiencing a major pregnancy complication; Importance of education on heart health; The practicality of referral to a clinic after pregnancy complications; Willingness for regular postpartum clinic visits after pregnancy complications. A thematic qualitative analysis was undertaken. Results: 26 women participated in four FGDs. The majority of women did not know of the association between major pregnancy complications and CVD. The main views expressed were: Women who experience pregnancy complications should receive education on improving heart health; An appointment for the first CVD risk screening visit needs to be made prior to discharge from the delivery suite; Women will benefit by having the option to select between a hospital and a general-practitioner based model of follow up. Conclusions: These views are important in developing postpartum strategies to reduce CVD risk among women who experience pregnancy complications. [ABSTRACT FROM AUTHOR]

Background: Publications may be a modifiable factor toward research project grant (RPG) funding decisions, the objective was to determine the association of publication record with later RPG receipt. Methods: This was a retrospective cohort study of recipients of K01, K08, or K23 US career development awards (CDAs) starting from 2000–2015. Exposures were CDA awardees' first-, middle-, and last-author publication counts, and the quartile of awardees' highest and mean publication impact factors. The independent association of each exposure with time to RPG (R01 or equivalent) was determined using a Cox model, after adjustment for CDA type, awardee change in institution, and institutional CDA count. The proportion of CDA recipients with later independent funding was also determined by publication count. Results: Among 6744 CDA awardees, 3943 obtained an RPG. The median time to RPG was 5.6 years (interquartile range 4.2–7.5). The number of first-authorships was associated with a shorter time to RPG (1–4 versus 0: hazard ratio [HR] 1.22, 95% confidence interval [CI] 1.10–1.36; 5–9: 1.59, 95% CI 1.40–1.79; 10–24: 1.78, 95% CI 1.54–2.07; 25+: 2.40, 95% CI 1.61–3.56). Last-authorships were associated with a shorter time to RPG (1–4 versus 0: HR 1.99, 95% CI 1.83–2.16; 5–9: 2.72, 95% CI 2.45–3.03; 10–24: 3.17, 95% CI 2.78–3.62; 25+: 3.12, 95% CI 2.17–4.50). Higher maximum impact factor was associated with a shorter time to RPG (Q2 versus lowest: HR 1.28, 95% CI 1.12–1.46; Q3: 1.45, 95% CI 1.24–1.70; Q4: 1.67, 95% CI 1.39–2.02). Mean impact factor and middle-authorships were not associated with time to RPG. Among 687 CDAs with zero associated first- or last-authorships, 158 (23%) achieved later RPG funding. Among those with at least 10 total first- or last-authorships, 1288/1554 (83%) obtained a later RPG. Conclusions: A higher number and impact of publications was associated with later independent funding. [ABSTRACT FROM AUTHOR]

Micro-anatomical reentry has been identified as a potential driver of atrial fibrillation (AF). In this paper, we introduce a novel computational method which aims to identify which atrial regions are most susceptible to micro-reentry. The approach, which considers the structural basis for micro-reentry only, is based on the premise that the accumulation of electrically insulating interstitial fibrosis can be modelled by simulating percolation-like phenomena on spatial networks. Our results suggest that at high coupling, where micro-reentry is rare, the micro-reentrant substrate is highly clustered in areas where the atrial walls are thin and have convex wall morphology, likely facilitating localised treatment via ablation. However, as transverse connections between fibres are removed, mimicking the accumulation of interstitial fibrosis, the substrate becomes less spatially clustered, and the bias to forming in thin, convex regions of the atria is reduced, possibly restricting the efficacy of localised ablation. Comparing our algorithm on image-based models with and without atrial fibre structure, we find that strong longitudinal fibre coupling can suppress the micro-reentrant substrate, whereas regions with disordered fibre orientations have an enhanced risk of micro-reentry. With further development, these methods may be useful for modelling the temporal development of the fibrotic substrate on an individualised basis. [ABSTRACT FROM AUTHOR]

Virtual perspective taking can reduce unconscious bias and increase empathy and prosocial behavior toward individuals who are marginalized based on group stereotypes such as age, race, or socioeconomic status. However, the question remains whether this approach might reduce implicit gender bias, and the degree to which virtual immersion contributes to behavioral modulation following perspective taking tasks is unknown. Accordingly, we investigate the role of virtual perspective taking for binary gender using an online platform (Study 1) and immersive virtual reality (Study 2). Female and male undergraduates performed a simulated interview while virtually represented by an avatar that was either congruent or incongruent with their own gender. All participants rated a male and a female candidate on competence, hireability, likeability, empathy, and interpersonal closeness and then chose one of these two equivalently qualified candidates to hire for a laboratory assistant position in the male dominated industry of information technology. Online perspective taking did not reveal a significant influence of avatar gender on candidate ratings or candidate choice, whereas virtual reality perspective taking resulted in significant changes to participant behavior following exposure to a gender-incongruent avatar (e.g., male embodied as female), such that men showed preference for the female candidate and women showed preference for the male candidate. Although between-group differences in candidate ratings were subtle, rating trends were consistent with substantial differences in candidate choice, and this effect was greater for men. Compared to an online approach, virtual reality perspective taking appears to exert greater influence on acute behavioral modulation for gender bias due to its ability to fully immerse participants in the experience of (temporarily) becoming someone else, with empathy as a potential mechanism underlying this phenomenon. [ABSTRACT FROM AUTHOR]

Open source analytical software for the analysis of electrophysiological cardiomyocyte data offers a variety of new functionalities to complement closed-source, proprietary tools. Here, we present the Cardio PyMEA application, a free, modifiable, and open source program for the analysis of microelectrode array (MEA) data obtained from cardiomyocyte cultures. Major software capabilities include: beat detection; pacemaker origin estimation; beat amplitude and interval; local activation time, upstroke velocity, and conduction velocity; analysis of cardiomyocyte property-distance relationships; and robust power law analysis of pacemaker spatiotemporal instability. Cardio PyMEA was written entirely in Python 3 to provide an accessible, integrated workflow that possesses a user-friendly graphical user interface (GUI) written in PyQt5 to allow for performant, cross-platform utilization. This application makes use of object-oriented programming (OOP) principles to facilitate the relatively straightforward incorporation of custom functionalities, e.g. power law analysis, that suit the needs of the user. Cardio PyMEA is available as an open source application under the terms of the GNU General Public License (GPL). The source code for Cardio PyMEA can be downloaded from Github at the following repository: https://github.com/csdunhamUC/cardio_pymea. [ABSTRACT FROM AUTHOR]