1. Comparison of participant-collected nasal and staff-collected oropharyngeal specimens for human ribonuclease P detection with RT-PCR during a community-based study.
- Author
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Arnold, Mitchell T., Temte, Jonathan L., Barlow, Shari K., Bell, Cristalyne J., Goss, Maureen D., Temte, Emily G., Checovich, Mary M., Reisdorf, Erik, Scott, Samantha, Guenther, Kyley, Wedig, Mary, Shult, Peter, and Uzicanin, Amra
- Subjects
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ACUTE diseases , *RESPIRATORY diseases , *REVERSE transcriptase - Abstract
We analyzed 4,352 participant- and staff-collected respiratory specimens from 2,796 subjects in the Oregon Child Absenteeism due to Respiratory Disease Study. Trained staff collected oropharyngeal specimens from school-aged children with acute respiratory illness while household participants of all ages collected their own midturbinate nasal specimens in year one and anterior nasal specimens in year two. Human ribonuclease P levels were measured using RT-PCR for all staff- and participant-collected specimens to determine adequacy, defined as Cycle threshold less than 38. Overall, staff- and participant-collected specimens were 99.9% and 96.4% adequate, respectively. Participant-collected midturbinate specimens were 95.2% adequate in year one, increasing to 97.2% in year two with anterior nasal collection. The mean human ribonuclease P Cycle threshold for participant-collected specimens was 31.18 in year one and 28.48 in year two. The results from this study suggest that community-based participant collection of respiratory specimens is comparable to staff-collected oropharyngeal specimens, is feasible, and may be optimal with anterior nasal collection. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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