1. Phosphorus-mediated alleviation of aluminum toxicity revealed by the iTRAQ technique in Citrus grandis roots
- Author
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Li-Song Chen, Bing Qian, Yang-Fei Zhou, Heng Wang, Lin-Tong Yang, Yan-Mei Wu, and Yan-Yu Wang
- Subjects
0106 biological sciences ,0301 basic medicine ,Citrus ,Physiology ,medicine.medical_treatment ,Cell Membranes ,Protein metabolism ,Malates ,Plant Science ,Reductase ,Toxicology ,Pathology and Laboratory Medicine ,01 natural sciences ,Biochemistry ,Lignin ,Plant Roots ,Starches ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,Plant Resistance to Abiotic Stress ,Medicine and Health Sciences ,Biomass ,Protein Metabolism ,Cellular Stress Responses ,chemistry.chemical_classification ,Principal Component Analysis ,Multidisciplinary ,Ecology ,Organic Compounds ,Phosphoribosyl pyrophosphate ,Eukaryota ,Phosphorus ,Starch ,Plants ,Chemistry ,Experimental Organism Systems ,Cell Processes ,Plant Physiology ,Isotope Labeling ,Shoot ,Toxicity ,Physical Sciences ,Medicine ,Growth inhibition ,Cellular Structures and Organelles ,Research Article ,Science ,Carbohydrates ,Research and Analysis Methods ,Thiobarbituric Acid Reactive Substances ,Citric Acid ,03 medical and health sciences ,Plant and Algal Models ,Plant-Environment Interactions ,medicine ,Plant Defenses ,Grasses ,Secretion ,Glutathione Peroxidase ,Protease ,Plant Ecology ,Ecology and Environmental Sciences ,Organic Chemistry ,Organisms ,Chemical Compounds ,Biology and Life Sciences ,Membrane Proteins ,Cell Biology ,Hydrogen Peroxide ,Plant Pathology ,030104 developmental biology ,Metabolism ,Glucose ,chemistry ,Animal Studies ,Rice ,Physiological Processes ,Phosphoenolpyruvate Carboxykinase (ATP) ,010606 plant biology & botany ,Organic acid ,Aluminum - Abstract
Citrus grandis seedlings were irrigated with nutrient solutions with four Al-P combinations [two Al levels (0 mM and 1.2 mM AlCl3·6H2O) × two P levels (0 μM and 200 μM KH2PO4)] for 18 weeks. Al dramatically inhibited the growth of C. grandis seedlings, as revealed by a decreased dry weight of roots and shoots. Elevating P level could ameliorate the Al-induced growth inhibition and organic acid (malate and citrate) secretion in C. grandis. Using a comparative proteomic approach revealed by the isobaric tags for relative and absolute quantification (iTRAQ) technique, 318 differentially abundant proteins (DAPs) were successfully identified and quantified in this study. The possible mechanisms underlying P-induced alleviation of Al toxicity in C. grandis were proposed. Furthermore, some DAPs, such as GLN phosphoribosyl pyrophosphate amidotransferase 2, ATP-dependent caseinolytic (Clp) protease/crotonase family protein, methionine-S-oxide reductase B2, ABC transporter I family member 17 and pyridoxal phosphate phosphatase, were reported for the first time to respond to Al stress in Citrus plants. Our study provides some proteomic details about the alleviative effects of P on Al toxicity in C. grandis, however, the exact function of the DAPs identified herein in response to Al tolerance in plants must be further investigated.
- Published
- 2019