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Brucellosis is a zoonotic disease imposing significant impacts on livestock production and public health worldwide. India is the world’s leading milk producer and Punjab is the state which produces the most cattle and buffalo milk per capita. The aim of this study was to investigate the epidemiology of bovine brucellosis to provide evidence for control of the disease in Punjab State, India. A cross-sectional study of dairy farms was conducted in humans and livestock in rural Ludhiana district using a multi-stage sampling strategy. The study suggests that brucellosis is endemic at high levels in cattle and buffalo in the study area with 15.1% of large ruminants testing seropositive and approximately a third of dairy farms having at least one animal test seropositive. In total, 9.7% of those in direct contact with livestock tested seropositive for Brucella spp. Persons that assisted with calving and/or abortion within the last year on a farm with seronegative livestock and people which did not assist with calving/abortion had 0.35 (95% CI: 0.17 to 7.1) and 0.21 (0.09 to 0.46) times the odds of testing seropositive compared to persons assisting with calving/abortion in a seropositive farm, respectively. The study demonstrated that persons in direct contact with cattle and buffalo in the study area have high risk of exposure to Brucella spp. Control of the disease in livestock is likely to result in benefits to both animal and public health sectors., Author summary Brucellosis is a bacterial disease that causes production losses in livestock due to abortions, increased calving intervals and reduced milk production. The disease can also be transmitted to humans via direct contact with livestock when they give birth or abort or via consumption of unpasteurised dairy products. This study aimed to estimate the frequency of exposure to the bacteria (Brucella spp.) in cattle and buffalo in Ludhiana district, Punjab State, India. In addition, persons in contact with these livestock, either through their occupation or household, were also tested for Brucella spp. antibodies and the data used to identify factors increasing their risk of exposure. The study found high levels of exposure in cattle and buffalo in the study area with 15.1% of large ruminants testing positive for antibodies to Brucella spp. (seropositive) and approximately a third of dairy farms having at least one animal test seropositive. Around 10% of people tested seropositive for the bacteria and those that assisted with calving and/or abortion on a farm where at least one animal tested seropositive had a high risk of infection. Control of brucellosis could reduce production losses in livestock and protect humans in contact with livestock from becoming infected.

Background Control operations targeting Animal African Trypanosomiasis and its primary vector, the tsetse, were covering approximately 128,000 km2 of Africa in 2001, which is a mere 1.3% of the tsetse infested area. Although extensive trypanosomiasis and tsetse (T&T) control operations have been running since the beginning of the 20th century, Animal African Trypanosomiasis is still a major constraint of livestock production in sub-Saharan Africa. Methodology/Principal Findings We performed a systematic review of the existing literature describing T&T control programmes conducted in a selection of five African countries, namely Burkina Faso, Cameroon, Ethiopia, Uganda and Zambia, between 1980 and 2015. Sixty-eight documents were eventually selected from those identified by the database search. This was supplemented with information gathered through semi-structured interviews conducted with twelve key informants recruited in the study countries and selected based on their experience and knowledge of T&T control. The combined information from these two sources was used to describe the inputs, processes and outcomes from 23 major T&T control programmes implemented in the study countries. Although there were some data gaps, involvement of the target communities and sustainability of the control activities were identified as the two main issues faced by these programmes. Further, there was a lack of evaluation of these control programmes, as well as a lack of a standardised methodology to conduct such evaluations. Conclusions/Significance Past experiences demonstrated that coordinated and sustained control activities require careful planning, and evidence of successes, failures and setbacks from past control programmes represent a mine of information. As there is a lack of evaluation of these programmes, these data have not been fully exploited for the design, analyses and justification of future control programmes., Author Summary Despite decades of control efforts targeting Animal African Trypanosomiasis, its distribution has hardly receded and the situation in sub-Saharan Africa has recently been described as “serious and deteriorating”. This disease of livestock affects farmers’ livelihood and welfare, food security and poses a threat to public health. Our work was primarily based on a review of available information on present and past control programmes targeting Animal African Trypanosomiasis and its vector, the tsetse, supplemented with interviews with key informants. We described and contextualised 23 well documented programmes implemented in five countries (Burkina Faso, Cameroon, Ethiopia, Uganda and Zambia). We summarised and discussed the issues commonly faced by these programmes and identified settings and circumstances which are more favourable for successful control activities. This work can contribute to the design and implementation of future programmes by highlighting the knowledge gained from past activities and experiences of those involved, and making recommendations as to how this can be utilised further.

In Lao People’s Democratic Republic pigs are kept in close contact with families. Human risk of infection with pig zoonoses arises from direct contact and consumption of unsafe pig products. This cross-sectional study was conducted in Luang Prabang (north) and Savannakhet (central-south) Provinces. A total of 59 villages, 895 humans and 647 pigs were sampled and serologically tested for zoonotic pathogens including: hepatitis E virus (HEV), Japanese encephalitis virus (JEV) and Trichinella spiralis; In addition, human sera were tested for Taenia spp. and cysticercosis. Seroprevalence of zoonotic pathogens in humans was high for HEV (Luang Prabang: 48.6%, Savannakhet: 77.7%) and T. spiralis (Luang Prabang: 59.0%, Savannakhet: 40.5%), and lower for JEV (around 5%), Taenia spp. (around 3%) and cysticercosis (Luang Prabang: 6.1, Savannakhet 1.5%). Multiple correspondence analysis and hierarchical clustering of principal components was performed on descriptive data of human hygiene practices, contact with pigs and consumption of pork products. Three clusters were identified: Cluster 1 had low pig contact and good hygiene practices, but had higher risk of T. spiralis. Most people in cluster 2 were involved in pig slaughter (83.7%), handled raw meat or offal (99.4%) and consumed raw pigs’ blood (76.4%). Compared to cluster 1, cluster 2 had increased odds of testing seropositive for HEV and JEV. Cluster 3 had the lowest sanitation access and had the highest risk of HEV, cysticercosis and Taenia spp. Farmers which kept their pigs tethered (as opposed to penned) and disposed of manure in water sources had 0.85 (95% CI: 0.18 to 0.91) and 2.39 (95% CI: 1.07 to 5.34) times the odds of having pigs test seropositive for HEV, respectively. The results have been used to identify entry-points for intervention and management strategies to reduce disease exposure in humans and pigs, informing control activities in a cysticercosis hyper-endemic village., Author Summary In Lao PDR, pigs are an important source of food and income and are kept by many rural residents. This study investigated five diseases that are transmitted between pigs and humans (zoonoses), namely hepatitis E, Japanese encephalitis, trichinellosis, cysticercosis and taeniasis. Humans and pigs in Lao PDR were tested for antibodies against the agents (pathogens) responsible for these diseases. Human participants were classified into three groups or "clusters" based on hygiene and sanitation practices, pig contact and pork consumption. Cluster 1 had low pig contact and good hygiene practice. Cluster 2 had moderate hygiene practices: around half used toilets and protected water sources; most people washed their hands after using the toilet and boiled water prior to consumption. Most people in this cluster were involved in pig slaughtering, drank pigs’ blood and were more likely test positive for antibodies against hepatitis E and Japanese encephalitis viruses. Finally, people in cluster 3 had lowest access to sanitation facilities, were most likely to have pigs in the household and had the highest risk of hepatitis E, taeniasis and cysticercosis. The diseases in this study pose a significant threat to public health and impact pig production. This study identified characteristics of high-risk individuals and areas with high disease burden and could be used to target future disease control activities to those most vulnerable.