1. Identification and Expression of the Multidrug and Toxic Compound Extrusion (MATE) Gene Family in Capsicum annuum and Solanum tuberosum
- Author
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Tian Hu, Shi Xiao, Ming Xiao, Hua Qi, Ying Zhou, Xue Zhang, Dai Yangshuo, Yuxin Wang, Qin-Fang Chen, Lu-Jun Yu, Linna Wang, Di Liu, and Sirui Ma
- Subjects
Capsicum annuum ,0106 biological sciences ,0301 basic medicine ,Plant Science ,Biology ,01 natural sciences ,Article ,03 medical and health sciences ,Expression profile ,lcsh:Botany ,Pepper ,Gene family ,Gene ,Ecology, Evolution, Behavior and Systematics ,reproductive and urinary physiology ,Solanaceae ,Segmental duplication ,Genetics ,Ecology ,Phylogenetic tree ,fungi ,food and beverages ,Solanum tuberosum ,biology.organism_classification ,lcsh:QK1-989 ,030104 developmental biology ,behavior and behavior mechanisms ,MATE ,Tandem exon duplication ,010606 plant biology & botany - Abstract
Multidrug and Toxic Compound Extrusion (MATE) proteins are essential transporters that extrude metabolites and participate in plant development and the detoxification of toxins. Little is known about the MATE gene family in the Solanaceae, which includes species that produce a broad range of specialized metabolites. Here, we identified and analyzed the complement of MATE genes in pepper (Capsicum annuum) and potato (Solanum tuberosum). We classified all MATE genes into five groups based on their phylogenetic relationships and their gene and protein structures. Moreover, we discovered that tandem duplication contributed significantly to the expansion of the pepper MATE family, while both tandem and segmental duplications contributed to the expansion of the potato MATE family, indicating that MATEs took distinct evolutionary paths in these two Solanaceous species. Analysis of &omega, values showed that all potato and pepper MATE genes experienced purifying selection during evolution. In addition, collinearity analysis showed that MATE genes were highly conserved between pepper and potato. Analysis of cis-elements in MATE promoters and MATE expression patterns revealed that MATE proteins likely function in many stages of plant development, especially during fruit ripening, and when exposed to multiple stresses, consistent with the existence of functional differentiation between duplicated MATE genes. Together, our results lay the foundation for further characterization of pepper and potato MATE gene family members.
- Published
- 2020
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