1. Isolation, characterization, and antitumor activity of a novel heteroglycan from cultured mycelia of Cordyceps sinensis
- Author
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Nan Peng, Hai Zhu, Yunxiang Liang, Pei-xin Zhu, Yuxia Mei, and Wei Yang
- Subjects
Magnetic Resonance Spectroscopy ,Pharmaceutical Science ,Antineoplastic Agents ,Apoptosis ,Fungus ,Polysaccharide ,Gas Chromatography-Mass Spectrometry ,Analytical Chemistry ,Residue (chemistry) ,Polysaccharides ,Carcinoma, Non-Small-Cell Lung ,Drug Discovery ,Spectroscopy, Fourier Transform Infrared ,Bioassay ,Animals ,Sarcoma 180 ,Mycelium ,Cell Proliferation ,Pharmacology ,chemistry.chemical_classification ,Cordyceps ,biology ,Organic Chemistry ,Nuclear magnetic resonance spectroscopy ,biology.organism_classification ,Complementary and alternative medicine ,Biochemistry ,chemistry ,Molecular Medicine - Abstract
A novel heteroglycan, Cordyceps sinensis polysaccharide 1 (molecular weight 1 17 × 105 Da), was isolated and purified from mycelia of the fungus C. sinensis obtained by solid-state culture. Structural characterization by chemical analysis, GC-MS, FTIR, and NMR spectroscopy showed that C. sinensis polysaccharide 1 was mainly composed of (1 → 6)-linked α-D-Glc and α-D-Gal, with minor β-(1 → 4)-D-Xyl and β-(1 → 4)-D-Man residues probably located in the side chains with a trace amount of α-(1 → 3)-L-Rha residue. In biological assays, C. sinensis polysaccharide 1 significantly inhibited proliferation of sarcoma 180 cells and induced apoptosis in a dose-dependent manner. Further studies will elucidate the antitumor mechanism of C. sinensis polysaccharide 1 and promote its utilization for the development of novel, effective anticancer drugs.
- Published
- 2014