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Abstract: The localization of transcripts of the pollenspecific gene NTP303 has been determined by means of in situ hybridization in combination with confocal laser scanning microscopy. NTP303 transcripts were first detectable at the mid-bi-nucleate stage of pollen development and persisted even after the pollen tube had reached the ovary. The majority of the NTP303 transcripts were localized in the vegetative cell, and were predominantly present in the apex of the pollen tube. To study the mechanism of regulation, the genomic clone NTPg303 was isolated and characterized. NTPg303 belongs to a gene family of at least five members. No introns are present in its coding region. Regions matching the pollen-specific PB-element TGTGGTT (Twell et al. 1991) have been found.