15 results on '"Ying Sun"'
Search Results
2. OsNAC016 regulates plant architecture and drought tolerance by interacting with the kinases GSK2 and SAPK8.
- Author
-
Qi Wu, Yingfan Liu, Zizhao Xie, Bo Yu, Ying Sun, and Junli Huang
- Published
- 2022
- Full Text
- View/download PDF
3. Protein Phosphatase 2A B'α and B'β Protect Centromeric Cohesion during Meiosis I
- Author
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Yi-Hong Yang, Lin-Lin Yan, Cui-Xia Pu, He Zhang, Yu-Lan Zhang, Wan-Yue Xu, Xin-Yu Yu, Ying Sun, and Ying-Jie Gao
- Subjects
0106 biological sciences ,Cohesin complex ,Physiology ,Centromere ,Arabidopsis ,Cell Cycle Proteins ,Plant Science ,Meiocyte ,Biology ,Chromatids ,01 natural sciences ,Chromosome segregation ,Chromosome Segregation ,Genetics ,Sister chromatids ,Protein Phosphatase 2 ,Phosphorylation ,Anaphase ,Arabidopsis Proteins ,Reproduction ,Articles ,Cell biology ,Establishment of sister chromatid cohesion ,Meiosis ,Chromatid ,010606 plant biology & botany - Abstract
During meiosis, the stepwise release of sister chromatid cohesion is crucial for the equal distribution of genetic material to daughter cells, enabling generation of fertile gametophytes. However, the molecular mechanism that protects centromeric cohesion from release at meiosis I is unclear in Arabidopsis (Arabidopsis thaliana). Here, we report that the protein phosphatase 2A regulatory subunits B'α and B'β participate in the control of sister chromatid separation. The double mutant b'αβ exhibited severe male and female sterility, caused by the lack of a nucleus or presence of an abnormal nucleus in mature microspores and embryo sacs. 4′,6-Diamidino-2-phenylindole staining revealed unequal amounts of DNA in the mononuclear microspores. Transverse sections of the anthers revealed unevenly sized tetrads with or without a nucleus, suggesting a defect in meiocyte meiosis. An analysis of chromosome spreads showed that the sister chromatids separated prematurely at anaphase I in b'αβ. Immunoblotting showed that AtRECOMBINATION DEFECTIVE8 (AtREC8), a key member of the cohesin complex, was hyperphosphorylated in b'αβ anthers and pistils during meiosis but hypophosphorylated in the wild type. Furthermore, yeast two-hybrid and bimolecular fluorescence complementation assays showed that B'α and B'β interact specifically with AtREC8, AtSHUGOSHIN1 (AtSGO1), AtSGO2, and PATRONUS1. Given that B'α was reported to localize to the centromere in meiotic cells, we propose that protein phosphatase 2A B'α and B'β are recruited by AtSGO1/2 and PATRONUS1 to dephosphorylate AtREC8 at the site of centromere cohesion to shield it from cleavage until anaphase II, contributing to the balanced separation of sister chromatids at meiosis.
- Published
- 2018
4. TRICHOME BIREFRINGENCE-LIKE27 Affects Aluminum Sensitivity by Modulating the O-Acetylation of Xyloglucan and Aluminum-Binding Capacity in Arabidopsis
- Author
-
Ying Sun, Nasim Mansoori, Yu Liu, Zhi Wei Wang, Bao Cai Zhang, Yuan Zhi Shi, Xiao Fang Zhu, Shao Jian Zheng, Yi Hua Zhou, and Jiang Xue Wan
- Subjects
inorganic chemicals ,food.ingredient ,Pectin ,Physiology ,Mutant ,Arabidopsis ,Plant Science ,complex mixtures ,Plant Roots ,Cell wall ,chemistry.chemical_compound ,food ,Cell Wall ,Polysaccharides ,Genetics ,Arabidopsis thaliana ,Hemicellulose ,Glucans ,biology ,Arabidopsis Proteins ,Holliday Junction Resolvases ,Acetylation ,Articles ,biology.organism_classification ,Xyloglucan ,chemistry ,Biochemistry ,Xylans ,Silique ,Aluminum - Abstract
Xyloglucan (XyG) has been reported to contribute to the aluminum (Al)-binding capacity of the cell wall in Arabidopsis (Arabidopsis thaliana). However, the influence of O-acetylation of XyG, accomplished by the putative O-acetyltransferase TRICHOME BIREFRINGENCE-LIKE27 (TBL27 [AXY4]), on its Al-binding capacity is not known. In this study, we found that the two corresponding TBL27 mutants, axy4-1 and axy4-3, were more Al sensitive than wild-type Columbia-0 plants. TBL27 was expressed in roots as well as in leaves, stems, flowers, and siliques. Upon Al treatment, even within 30 min, TBL27 transcript accumulation was strongly down-regulated. The mutants axy4-1 and axy4-3 accumulated significantly more Al in the root and wall, which could not be correlated with pectin content or pectin methylesterase activity, as no difference in the mutants was observed compared with the wild type when exposed to Al stress. The increased Al accumulation in the wall of the mutants was found to be in the hemicellulose fraction. While the total sugar content of the hemicellulose fraction did not change, the O-acetylation level of XyG was reduced by Al treatment. Taken together, we conclude that modulation of the O-acetylation level of XyG influences the Al sensitivity in Arabidopsis by affecting the Al-binding capacity in the hemicellulose.
- Published
- 2014
5. Xyloglucan Endotransglucosylase-Hydrolase17 Interacts with Xyloglucan Endotransglucosylase-Hydrolase31 to Confer Xyloglucan Endotransglucosylase Action and Affect Aluminum Sensitivity in Arabidopsis
- Author
-
Shao Jian Zheng, Ying Sun, Janet Braam, Xiao Fang Zhu, Yuan Zhi Shi, Jiang Xue Wan, and Gui Xin Li
- Subjects
inorganic chemicals ,biology ,Physiology ,Mutant ,Saccharomyces cerevisiae ,Wild type ,Articles ,Plant Science ,Xyloglucan endotransglucosylase ,biology.organism_classification ,complex mixtures ,Cell biology ,Cell wall ,Xyloglucan ,chemistry.chemical_compound ,chemistry ,Biochemistry ,Arabidopsis ,Genetics ,Arabidopsis thaliana ,sense organs - Abstract
Previously, we reported that although the Arabidopsis (Arabidopsis thaliana) Xyloglucan Endotransglucosylase-Hydrolase31 (XTH31) has predominately xyloglucan endohydrolase activity in vitro, loss of XTH31 results in remarkably reduced in vivo xyloglucan endotransglucosylase (XET) action and enhanced Al resistance. Here, we report that XTH17, predicted to have XET activity, binds XTH31 in yeast (Saccharomyces cerevisiae) two-hybrid and coimmunoprecipitations assays and that this interaction may be required for XTH17 XET activity in planta. XTH17 and XTH31 may be colocalized in plant cells because tagged XTH17 fusion proteins, like XTH31 fusion proteins, appear to target to the plasma membrane. XTH17 expression, like that of XTH31, was substantially reduced in the presence of aluminum (Al), even at concentrations as low as 10 µm for 24 h or 25 µm for just 30 min. Agrobacterium tumefaciens-mediated transfer DNA insertion mutant of XTH17, xth17, showed low XET action and had moderately shorter roots than the wild type but was more Al resistant than the wild type. Similar to xth31, xth17 had low hemicellulose content and retained less Al in the cell wall. These data suggest a model whereby XTH17 and XTH31 may exist as a dimer at the plasma membrane to confer in vivo XET action, which modulates cell wall Al-binding capacity and thereby affects Al sensitivity in Arabidopsis.
- Published
- 2014
6. A Rice Virescent-Yellow Leaf Mutant Reveals New Insights into the Role and Assembly of Plastid Caseinolytic Protease in Higher Plants
- Author
-
Yu-Ying Sun, Ling Jiang, Yulong Ren, Zhijun Cheng, Cailin Lei, Chuanyin Wu, Gui-Lin Fei, Hui Dong, Jianmin Wan, Haiyang Wang, Mingjiang Chen, Ning Su, Jiulin Wang, Xin Zhang, Xiuping Guo, Kunneng Zhou, and Fuqing Wu
- Subjects
Chlorophyll ,Chloroplasts ,Positional cloning ,Physiology ,Mutant ,Plant Science ,Biology ,Protein degradation ,Biochemistry and Metabolism ,Gene Expression Regulation, Plant ,Two-Hybrid System Techniques ,Arabidopsis ,Protein Interaction Mapping ,Genetics ,Arabidopsis thaliana ,Plastids ,Cloning, Molecular ,Photosynthesis ,Plastid ,Plant Proteins ,Tandem affinity purification ,Binding Sites ,fungi ,food and beverages ,Oryza ,Endopeptidase Clp ,Plants, Genetically Modified ,biology.organism_classification ,Cell biology ,Plant Leaves ,Chloroplast ,Phenotype - Abstract
The plastidic caseinolytic protease (Clp) of higher plants is an evolutionarily conserved protein degradation apparatus composed of a proteolytic core complex (the P and R rings) and a set of accessory proteins (ClpT, ClpC, and ClpS). The role and molecular composition of Clps in higher plants has just begun to be unraveled, mostly from studies with the model dicotyledonous plant Arabidopsis (Arabidopsis thaliana). In this work, we isolated a virescent yellow leaf (vyl) mutant in rice (Oryza sativa), which produces chlorotic leaves throughout the entire growth period. The young chlorotic leaves turn green in later developmental stages, accompanied by alterations in chlorophyll accumulation, chloroplast ultrastructure, and the expression of chloroplast development- and photosynthesis-related genes. Positional cloning revealed that the VYL gene encodes a protein homologous to the Arabidopsis ClpP6 subunit and that it is targeted to the chloroplast. VYL expression is constitutive in most tissues examined but most abundant in leaf sections containing chloroplasts in early stages of development. The mutation in vyl causes premature termination of the predicted gene product and loss of the conserved catalytic triad (serine-histidine-aspartate) and the polypeptide-binding site of VYL. Using a tandem affinity purification approach and mass spectrometry analysis, we identified OsClpP4 as a VYL-associated protein in vivo. In addition, yeast two-hybrid assays demonstrated that VYL directly interacts with OsClpP3 and OsClpP4. Furthermore, we found that OsClpP3 directly interacts with OsClpT, that OsClpP4 directly interacts with OsClpP5 and OsClpT, and that both OsClpP4 and OsClpT can homodimerize. Together, our data provide new insights into the function, assembly, and regulation of Clps in higher plants.
- Published
- 2013
7. Protein Phosphatase 2A B'α and B'β Protect Centromeric Cohesion during Meiosis I.
- Author
-
Yu-Lan Zhang, He Zhang, Ying-Jie Gao, Lin-Lin Yan, Xin-Yu Yu, Yi-Hong Yang, Wan-Yue Xu, Cui-Xia Pu, and Ying Sun
- Published
- 2019
- Full Text
- View/download PDF
8. Two rice authentic histidine phosphotransfer proteins, OsAHP1 and OsAHP2, mediate cytokinin signaling and stress responses in rice
- Author
-
Qian Zhang, Li-Jing Sun, Ying Sun, Zhiguo Zhang, Xue-Wen Jiao, Daye Sun, Sheng-Wei Zhang, Li-Qing Zhang, Jinxia Wu, and Tiegang Lu
- Subjects
Cytokinins ,Physiology ,Transgene ,Plant Development ,Plant Science ,Sodium Chloride ,Plant Roots ,chemistry.chemical_compound ,Cytosol ,RNA interference ,Osmotic Pressure ,Stress, Physiological ,Arabidopsis ,Botany ,Genetics ,Arabidopsis thaliana ,Histidine ,Phosphorylation ,Plant Proteins ,Cell Nucleus ,Oryza sativa ,biology ,Lateral root ,fungi ,food and beverages ,Oryza ,Articles ,biology.organism_classification ,Plants, Genetically Modified ,Cell biology ,Response regulator ,Phenotype ,chemistry ,Seedlings ,Cytokinin ,RNA Interference ,Signal Transduction - Abstract
Cytokinin plays an important role in plant development and stress tolerance. Studies of Arabidopsis (Arabidopsis thaliana) have demonstrated that cytokinin acts through a two-component system that includes a histidine (His) kinase, a His phosphotransfer protein (HP), and a response regulator. Phylogenetic analyses have revealed the conservation of His kinases but lineage-specific expansion of HPs and response regulators in rice (Oryza sativa). However, whether the functions of rice HPs have diverged remains unknown. In this study, two rice authentic HPs (OsAHP1 and OsAHP2) were knocked down simultaneously via RNA interference (RNAi), and the transgenic OsAHP- RNAi plants exhibited phenotypes expected for a deficiency in cytokinin signaling, including dwarfism with reduced internode lengths, enhanced lateral root growth, early leaf senescence, and reduced tiller numbers and fertility under natural conditions. The OsAHP- RNAi seedlings were also hyposensitive to exogenous cytokinin. Furthermore, OsAHP- RNAi seedlings were hypersensitive to salt treatment but resistant to osmotic stress relative to wild-type plants. These results indicate that OsAHPs function as positive regulators of the cytokinin signaling pathway and play different roles in salt and drought tolerance in rice.
- Published
- 2014
9. Altered architecture and enhanced drought tolerance in rice via the down-regulation of indole-3-acetic acid by TLD1/OsGH3.13 activation
- Author
-
Chen-Hui Li, Jia Cao, Sheng-Wei Zhang, Yu-Feng Xia, Yong-Cun Zhang, Su-Qiao Zhang, Daye Sun, and Ying Sun
- Subjects
Physiology ,Drought tolerance ,Mutant ,Molecular Sequence Data ,Arabidopsis ,Down-Regulation ,Plant Science ,Biology ,Oryza ,Genes, Plant ,Models, Biological ,chemistry.chemical_compound ,Auxin ,Gene Expression Regulation, Plant ,Stress, Physiological ,Botany ,Genetics ,Cloning, Molecular ,Plant Proteins ,chemistry.chemical_classification ,Oryza sativa ,Base Sequence ,Indoleacetic Acids ,Gene Expression Profiling ,fungi ,Wild type ,Plant physiology ,food and beverages ,Reproducibility of Results ,biology.organism_classification ,Adaptation, Physiological ,Cell biology ,Droughts ,Phenotype ,chemistry ,Seedlings ,Mutation ,Indole-3-acetic acid ,Research Article - Abstract
Plant architecture is determined by genetic and developmental programs as well as by environmental factors. Sessile plants have evolved a subtle adaptive mechanism that allows them to alter their growth and development during periods of stress. Phytohormones play a central role in this process; however, the molecules responsible for integrating growth- and stress-related signals are unknown. Here, we report a gain-of-function rice (Oryza sativa) mutant, tld1-D, characterized by (and named for) an increased number of tillers, enlarged leaf angles, and dwarfism. TLD1 is a rice GH3.13 gene that encodes indole-3-acetic acid (IAA)-amido synthetase, which is suppressed in aboveground tissues under normal conditions but which is dramatically induced by drought stress. The activation of TLD1 reduced the IAA maxima at the lamina joint, shoot base, and nodes, resulting in subsequent alterations in plant architecture and tissue patterning but enhancing drought tolerance. Accordingly, the decreased level of free IAA in tld1-D due to the conjugation of IAA with amino acids greatly facilitated the accumulation of late-embryogenesis abundant mRNA compared with the wild type. The direct regulation of such drought-inducible genes by changes in the concentration of IAA provides a model for changes in plant architecture via the process of drought adaptation, which occurs frequently in nature.
- Published
- 2009
10. TRICHOME BIREFRINGENCE-LIKE27 Affects Aluminum Sensitivity by Modulating the O-Acetylation of Xyloglucan and Aluminum-Binding Capacity in Arabidopsis.
- Author
-
Xiao Fang Zhu, Ying Sun, Bao Cai Zhang, Mansoori, Nasim, Jiang Xue Wan, Yu Liu, Zhi Wei Wang, Yuan Zhi Shi, Yi Hua Zhou, and Shao Jian Zheng
- Subjects
- *
XYLOGLUCANS , *PLANT cell walls , *ARABIDOPSIS thaliana genetics , *TRICHOMES , *PECTINS - Abstract
Xyloglucan (XyG) has been reported to contribute to the aluminum (Al)-binding capacity of the cell wall in Arabidopsis (Arabidopsis thaliana). However, the influence of O-acetylation of XyG, accomplished by the putative O-acetyltransferase TRICHOME BIREFRINGENCE-LIKE27 (TBL27 [AXY4]), on its Al-binding capacity is not known. In this study, we found that the two corresponding TBL27 mutants, axy4-1 and axy4-3, were more Al sensitive than wild-type Columbia-0 plants. TBL27 was expressed in roots as well as in leaves, stems, flowers, and siliques. Upon Al treatment, even within 30 min, TBL27 transcript accumulation was strongly down-regulated. The mutants axy4-1 and axy4-3 accumulated significantly more Al in the root and wall, which could not be correlated with pectin content or pectin methylesterase activity, as no difference in the mutants was observed compared with the wild type when exposed to Al stress. The increased Al accumulation in the wall of the mutants was found to be in the hemicellulose fraction. While the total sugar content of the hemicellulose fraction did not change, the O-acetylation level of XyG was reduced by Al treatment. Taken together, we conclude that modulation of the O-acetylation level of XyG influences the Al sensitivity in Arabidopsis by affecting the Al-binding capacity in the hemicellulose. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
11. Xyloglucan Endotransglucosylase-Hydrolase17 Interacts with Xyloglucan Endotransglucosylase-Hydrolase31 to Confer Xyloglucan Endotransglucosylase Action and Affect Aluminum Sensitivity in Arabidopsis.
- Author
-
Xiao Fang Zhu, Jiang Xue Wan, Ying Sun, Yuan Zhi Shi, Janet Braam, Gui Xin Li, and Shao Jian Zheng
- Subjects
ARABIDOPSIS thaliana ,XYLOGLUCANS ,SACCHAROMYCES cerevisiae ,YEAST research ,PROTEIN research - Abstract
Previously, we reported that although the Arabidopsis (Arabidopsis thaliana) Xyloglucan Endotransglucosylase-Hydrolase31 (XTH31) has predominately xyloglucan endohydrolase activity in vitro, loss of XTH31 results in remarkably reduced in vivo xyloglucan endotransglucosylase (XET) action and enhanced A1 resistance. Here, we report that XTH17, predicted to have XET activity, binds XTH31 in yeast (Saccharomyces cerevisiae) two-hybrid and coimmunoprecipitations assays and that this interaction may be required for XTH17 XET activity in planta. XTH17 and XTH31 may be colocalized in plant cells because tagged XTH17 fusion proteins, like XTH31 fusion proteins, appear to target to the plasma membrane. XTH17 expression, like that of XTH3I, was substantially reduced in the presence of aluminum (A1), even at concentrations as low as 10 µM for 24 h or 25 µM for just 30 min. Agrobacterium tumefaciens-mediated transfer DNA insertion mutant of XTH17, xth17, showed low XET action and had moderately shorter roots than the wild type but was more A1 resistant than the wild type. Similar to xth31, xthl7 had low hemicellulose content and retained less A1 in the cell wall. These data suggest a model whereby XTH17 and XTH31 may exist as a dimer at the plasma membrane to confer in vivo XET action, which modulates cell wall Al-binding capacity and thereby affects A1 sensitivity in Arabidopsis. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
12. Two Rice Authentic Histidine Phosphotransfer Proteins, OsAHP1 and OsAHP2, Mediate Cytokinin Signaling and Stress Responses in Rice.
- Author
-
Lijing Sun, Qian Zhang, Jinxia Wu, Liqing Zhang, Xuewen Jiao, Shengwei Zhang, Zhiguo Zhang, Daye Sun, Tiegang Lu, and Ying Sun
- Subjects
HISTIDINE ,CYTOKININS ,ARABIDOPSIS thaliana genetics ,PHOSPHOTRANSFERASES ,RICE genetics ,DROUGHT tolerance - Abstract
Cytokinin plays an important role in plant development and stress tolerance. Studies of Arabidopsis (Arabidopsis thaliana) have demonstrated that cytokinin acts through a two-component system that includes a histidine (His) kinase, a His phosphotransfer protein (HP), and a response regulator. Phylogenetic analyses have revealed the conservation of His kinases but lineage-specific expansion of HPs and response regulators in rice (Oryza sativa). However, whether the functions of rice HPs have diverged remains unknown. In this study, two rice authentic HPs (OsAHP1 and OsAHP2) were knocked down simultaneously via RN interference (RNAi), and the transgenic OsAHP-RNAi plants exhibited phenotypes expected for a deficiency in cytokinin signaling, including dwarfism with reduced internode lengths, enhanced lateral root growth, early leaf senescence, and reduced tiller numbers and fertility under natural conditions. The OsAHP-RNAi seedlings were also hyposensitive to exogenous cytokinin. Furthermore, OsAHP-RNAi seedlings were hypersensitive to salt treatment but resistant to osmotic stress relative to wild-type plants. These results indicate that OsAHPs function as positive regulators of the cytokinin signaling pathway and play different roles in salt and drought tolerance in rice. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
13. A Rice Virescent-Yellow Leaf Mutant Reveals New Insights into the Role and Assembly of Plastid Caseinolytic Protease in Higher Plants.
- Author
-
Hui Dong, Gui-Lin Fei, Chuan-Yin Wu, Fu-Qing Wu, Yu-Ying Sun, Ming-Jiang Chen, Yu-Long Ren, Kun-Neng Zhou, Zhi-Jun Cheng, Jiu-Lin Wang, Ling Jiang, Xin Zhang, Xiu-Ping Guo, Cai-Lin Lei, Ning Su, Haiyang Wang, and Jian-Min Wan
- Subjects
RICE ,ORYZA ,LEAVES ,BOTANY ,PROTEOLYTIC enzymes - Abstract
The plastidic caseinolytic protease (CIp) of higher plants is an evolutionarily conserved protein degradation apparatus composed of a proteolytic core complex (the P and R rings) and a set of accessory proteins (ClpT, ClpC, and CIpS). The role and molecular composition of Clps in higher plants has just begun to be unraveled, mostly from studies with the model dicotyledonous plant Arabidopsis (Arabidopsis thaliana). In this work, we isolated a virescent yellow leaf (vyl) mutant in rice (Oryza sativa), which produces chlorotic leaves throughout the entire growth period. The young chlorotic leaves turn green in later developmental stages, accompanied by alterations in chlorophyll accumulation, chloroplast ultrastructure, and the expression of chloroplast development- and photosynthesis-related genes. Positional cloning revealed that the VYL gene encodes a protein homologous to the Arabidopsis ClpP6 subtmit and that it is targeted to the chloroplast. VYL expression is constitutive in most tissues examined but most abundant in leaf sections containing chloroplasts in early stages of development. The mutation in wdl causes premature termination of the predicted gene product and loss of the conserved catalytic triad (serine-histidine-aspartate) and the polypeptide-binding site of VYL. Using a tandem affinity purification approach and mass spectrometry analysis, we identified OsCIpP4 as a VYL-associated protein in vivo. In addition, yeast two-hybrid assays demonstrated that VYL directly interacts with OsClpP3 and OsClpP4. Furthermore, we found that OsCIpP3 directly interacts with OsClpT, that OsClpP4 directly interacts with OsClpP5 and OsCIpT, and that both OsClpP4 and OsClpT can homodimerize. Together, our data provide new insights into the function, assembly, and regulation of Clps in higher plants. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
14. The Rice Wall-Associated Receptor-Like Kinase Gene OsDEES1 Plays a Role in Female Gametophyte Development.
- Author
-
Na Wang, Hui-Jia Huang, Su-Ting Ren, Jiao-Jiao Li, Ying Sun, Da-Ye Sun, and Su-Qiao Zhang
- Subjects
RECEPTOR-like kinases ,GAMETOPHYTES ,ARABIDOPSIS thaliana ,RICE ,PLANT genetics - Abstract
The wall-associated kinase (WAK) gene family is a unique subfamily of receptor-like kinases (RLKs) in plants. WAK-RLKs play roles in cell expansion, pathogen resistance, and metal tolerance in Arabidopsis (Arabidopsis thaliana). Rice (Oryza sativa) has far more WAK-RLK genes than Arabidopsis, but the functions of rice WAK-RLKs are poorly understood. In this study, we found that one rice WAK-RLK gene, DEFECT IN EARLY EMBRYO SAC1 (OsDEES1), is involved in the regulation of early embryo sac development. OsDEES1 silencing by RNA interference caused a high rate of female sterility. Crossing experiments showed that female reproductive organs lacking OsDEES1 carried a functional defect. A detailed investigation of the ovaries from OsDEES1 RNA interference plants indicated that the knockdown of OsDEES1 expression did not affect megasporogenesis but that it disturbed female gametophyte formation, resulting in a degenerated embryo sac and defective seed formation. OsDEES1 exhibited a tissue-specific expression pattern in flowers and seedlings. In the ovary, OsDEES1 was expressed in the megagametophyte region and surrounding nucellus cells in the ovule near the micropylar region. OsDEES1 was found to be a membrane-localized protein with a unique sequence compared with other WAK-RLKs. These data indicate that OsDEES1 plays a role in rice sexual reproduction by regulating female gametophyte development. This study offers new insight into the functions of the WAK-RLK family. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
15. Altered Architecture and Enhanced Drought Tolerance in Rice via the Down-Regulation of Indole-3-Acetic Acid by TLD1/OsGH3.13 Activation.
- Author
-
Sheng-Wei Zhang, Chen-Hui Li, Jia Cao, Yong-Cun Zhang, Su-Qiao Zhang, Yu-Feng Xia, Da-Ye Sun, and Ying Sun
- Subjects
RICE ,DROUGHTS & the environment ,PLANT hormones ,DWARFISM ,MOLECULES ,MESSENGER RNA ,PLANT development ,PHYSIOLOGY ,PLANTS - Abstract
Plant architecture is determined by genetic and developmental programs as well as by environmental factors. Sessile plants have evolved a subtle adaptive mechanism that allows them to alter their growth and development during periods of stress. Phytohormones play a central role in this process; however, the molecules responsible for integrating growth- and stress-related signals are unknown. Here, we report a gain-of-function rice (Oryza sativa) mutant, tld1-D, characterized by (and named for) an increased number of tillers, enlarged leaf angles, and dwarfism. TLD1 is a rice GH3.13 gene that encodes indole-3-acetic acid (IAA)-amido synthetase, which is suppressed in aboveground tissues under normal conditions but which is dramatically induced by drought stress. The activation of TLD1 reduced the IAA maxima at the lamina joint, shoot base, and nodes, resulting in subsequent alterations in plant architecture and tissue patterning but enhancing drought tolerance. Accordingly, the decreased level of free IAA in tld1-D due to the conjugation of IAA with amino acids greatly facilitated tha accumulation of late-embryogenesis abundant mRNA compared with the wild type. The direct regulation of such drought-inducible genes by changes in the concentration of IAA provides a model for changes in plant architecture via the process of drought adaptation which occurs frequently in nature. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
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