1. Endoplasmic reticulum-quality control chaperones facilitate the biogenesis of Cf receptor-like proteins involved in pathogen resistance of tomato.
- Author
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Liebrand TW, Smit P, Abd-El-Haliem A, de Jonge R, Cordewener JH, America AH, Sklenar J, Jones AM, Robatzek S, Thomma BP, Tameling WI, and Joosten MH
- Subjects
- Amino Acid Sequence, Cladosporium physiology, Gene Silencing, Glycosylation, Green Fluorescent Proteins isolation & purification, Molecular Sequence Data, Plant Leaves metabolism, Plant Proteins chemistry, Plant Proteins metabolism, Plants, Genetically Modified, Polysaccharides metabolism, Protein Binding, Recombinant Fusion Proteins isolation & purification, Recombinant Fusion Proteins metabolism, Sequence Homology, Amino Acid, Nicotiana genetics, Transformation, Genetic, Disease Resistance, Endoplasmic Reticulum metabolism, Solanum lycopersicum metabolism, Solanum lycopersicum microbiology, Molecular Chaperones metabolism, Plant Diseases microbiology, Plant Proteins biosynthesis
- Abstract
Cf proteins are receptor-like proteins (RLPs) that mediate resistance of tomato (Solanum lycopersicum) to the foliar pathogen Cladosporium fulvum. These transmembrane immune receptors, which carry extracellular leucine-rich repeats that are subjected to posttranslational glycosylation, perceive effectors of the pathogen and trigger a defense response that results in plant resistance. To identify proteins required for the functionality of these RLPs, we performed immunopurification of a functional Cf-4-enhanced green fluorescent protein fusion protein transiently expressed in Nicotiana benthamiana, followed by mass spectrometry. The endoplasmic reticulum (ER) heat shock protein70 binding proteins (BiPs) and lectin-type calreticulins (CRTs), which are chaperones involved in ER-quality control, were copurifying with Cf-4-enhanced green fluorescent protein. The tomato and N. benthamiana genomes encode four BiP homologs and silencing experiments revealed that these BiPs are important for overall plant viability. For the three tomato CRTs, virus-induced gene silencing targeting the plant-specific CRT3a gene resulted in a significantly compromised Cf-4-mediated defense response and loss of full resistance to C. fulvum. We show that upon knockdown of CRT3a the Cf-4 protein accumulated, but the pool of Cf-4 protein carrying complex-type N-linked glycans was largely reduced. Together, our study on proteins required for Cf function reveals an important role for the CRT ER chaperone CRT3a in the biogenesis and functionality of this type of RLP involved in plant defense.
- Published
- 2012
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