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Your search keyword '"Argüello-Astorga G"' showing total 2 results
Start Over Author "Argüello-Astorga G" Journal plant physiology
2 results on '"Argüello-Astorga G"'

1. Ancestral multipartite units in light-responsive plant promoters have structural features correlating with specific phototransduction pathways.

Author

Argüello-Astorga, G R and Herrera-Estrella, L R

Abstract

Regulation of plant gene transcription by light is mediated by multipartite cis-regulatory units. Previous attempts to identify structural features that are common to all light-responsive elements (LREs) have been unsuccessful. To address the question of what is needed to confer photoresponsiveness to a promoter, the upstream sequences from more than 110 light-regulated plant genes were analyzed by a new, phylogenetic-structural method. As a result, 30 distinct conserved DNA module arrays (CMAs) associated with light-responsive promoter regions were identified. Several of these CMAs have remained invariant throughout the evolutionary radiation of angiosperms and are conserved between homologous genes as well as between members of different gene families. The identified CMAs share a gene superfamily-specific core that correlates with the particular phytochrome-dependent transduction pathway that controls their expression, i.e. ACCTA(A/C)C(A/C) for the cGMP-dependent phenylpropanoid metabolism-associated genes, and GATA(A/T)GR for the Ca2+/calmodulin-dependent photosynthesis-associated nuclear genes. In addition to suggesting a general model for the functional and structural organization of LREs, the data obtained in this study indicate that angiosperm LREs probably evolved from complex cis-acting elements involved in regulatory processes other than photoregulation in gymnosperms.

Published
1996
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2. Functional properties and regulatory complexity of a minimal RBCS light-responsive unit activated by phytochrome, cryptochrome, and plastid signals.

Author

Martínez-Hernández A, López-Ochoa L, Argüello-Astorga G, and Herrera-Estrella L

Subjects
Arabidopsis metabolism, Arabidopsis radiation effects, Basic-Leucine Zipper Transcription Factors, Cryptochromes, Darkness, Gene Expression Regulation, Plant radiation effects, Glucuronidase genetics, Glucuronidase metabolism, Light, Mutation, Nuclear Proteins genetics, Plant Leaves genetics, Plant Leaves metabolism, Plant Leaves radiation effects, Plants, Genetically Modified, Receptors, G-Protein-Coupled, Response Elements genetics, Signal Transduction, Transcription, Genetic radiation effects, Arabidopsis genetics, Arabidopsis Proteins, Drosophila Proteins, Eye Proteins, Flavoproteins metabolism, Photoreceptor Cells, Invertebrate, Phytochrome metabolism, Plastids metabolism, Promoter Regions, Genetic genetics, Ribulose-Bisphosphate Carboxylase genetics
Abstract

Light-inducible promoters are able to respond to a wide spectrum of light through multiple photoreceptor systems. Several cis-acting elements have been identified as components of light-responsive promoter elements; however, none of these regulatory elements by itself appears to be sufficient to confer light responsiveness; rather, the combination of at least two elements seems to be required. Using phylogenetic structural analysis, we have identified conserved DNA modular arrays (CMAs) associated with light-responsive promoter regions that have been conserved throughout the evolutionary radiation of angiosperms. Here, we report the functional characterization of CMA5, a native 52-bp fragment of the Nicotiana plumbaginifolia rbcS 8B promoter, which contains an I- and a G-box cis-element. CMA5 behaves as a light-responsive minimal unit capable of activating a heterologous minimal promoter in a phytochrome-, cryptochrome-, and plastid-dependent manner. We also show that CMA5 light induction requires HY5 and that downstream negative regulators COP (constitutive photomorphogenic)/DET (de-etiolated) regulate its activity. Our results show that the simplest light-responsive promoter element from photosynthesis-associated genes described to date is the common target for different signals involved in light regulation. The possible mechanism involved in light-transcriptional regulation and tissue specificity of combinatorial elements units is discussed.

Published
2002
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