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8 results on '"Mylle, Evelien"'

5. TPX2-LIKE PROTEIN3 Is the Primary Activator of α-Aurora Kinases and Is Essential for Embryogenesis

Author

Boruc, Joanna, primary, Deng, Xingguang, additional, Mylle, Evelien, additional, Besbrugge, Nienke, additional, Van Durme, Matthias, additional, Demidov, Dmitri, additional, Tomaštíková, Eva Dvořák, additional, Tan, Tong-Reen Connie, additional, Vandorpe, Michaël, additional, Eeckhout, Dominique, additional, Beeckman, Tom, additional, Nowack, Moritz K., additional, De Jaeger, Geert, additional, Lin, Honghui, additional, Liu, Bo, additional, and Van Damme, Daniël, additional

Published
2019
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6. High Temporal Resolution Reveals Simultaneous Plasma Membrane Recruitment of TPLATE Complex Subunits.

Author

Jie Wang, Mylle, Evelien, Johnson, Alexander, Besbrugge, Nienke, De Jaeger, Geert, Friml, Jiří, Pleskot, Roman, and Van Damme, Daniel

Abstract

The TPLATE complex (TPC) is a key endocytic adaptor protein complex in plants. TPC in Arabidopsis (Arabidopsis thaliana) contains six evolutionarily conserved subunits and two plant-specific subunits (AtEH1/Pan1 and AtEH2/Pan1) which, although cytoplasmic proteins, are not associated with the hexameric subcomplex in the cytoplasm. To investigate the dynamic assembly of the octameric TPC at the plasma membrane (PM), we performed state-of-the-art dual-color live-cell imaging at physiological and lowered temperatures. Lowering the temperature slowed down endocytosis, thereby enhancing the temporal resolution of the differential recruitment of endocytic components. Under both normal and lowered temperature conditions, the core TPC subunit TPLATE and the AtEH/Pan1 proteins exhibited simultaneous recruitment at the PM. These results, together with colocalization analysis of different TPC subunits, allow us to conclude that the TPC in plant cells is not recruited to the PM sequentially but as an octameric complex. [ABSTRACT FROM AUTHOR]

Published
2020
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7. Phosphorylation of MAP65-1 by Arabidopsis Aurora Kinases Is Required for Efficient Cell Cycle Progression

Author

Boruc, Joanna, primary, Weimer, Annika K., additional, Stoppin-Mellet, Virginie, additional, Mylle, Evelien, additional, Kosetsu, Ken, additional, Cedeño, Cesyen, additional, Jaquinod, Michel, additional, Njo, Maria, additional, De Milde, Liesbeth, additional, Tompa, Peter, additional, Gonzalez, Nathalie, additional, Inzé, Dirk, additional, Beeckman, Tom, additional, Vantard, Marylin, additional, and Van Damme, Daniël, additional

Published
2016
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