1. The plant mitochondrial open reading frame orf221 encodes a membrane-bound protein
- Author
-
Charles S. Levings, Jintai Huang, and Laudenir M. Prioli
- Subjects
Mitochondrial DNA ,Recombinant Fusion Proteins ,Plant Science ,Biology ,Mitochondrion ,Cell Fractionation ,Genes, Plant ,MT-RNR1 ,DNA, Mitochondrial ,Zea mays ,MT-RNR2 ,Open Reading Frames ,Escherichia coli ,Genetics ,HSPA9 ,Membrane Proteins ,food and beverages ,General Medicine ,Plants ,Molecular biology ,Open reading frame ,Biochemistry ,Mitochondrial Membrane Protein ,Protein Biosynthesis ,ATP–ADP translocase ,Agronomy and Crop Science - Abstract
We have shown that the open reading frame orf221 is an active mitochondrial gene which encodes a novel mitochondrial polypeptide. The orf221 sequence is common to higher plants but absent in animal and fungal mitochondria. A mitochondrial polypeptide with an apparent molecular weight of 21,000 was detected with a polyclonal antibody raised against an ORF221 fusion protein. In organello translation followed by immunoprecipitation with the anti-ORF221 antibody demonstrated that this polypeptide is encoded by the orf221 gene in plant mitochondria. The ORF221 was found to be a mitochondrial membrane protein in normal (N), cms-T, and cms-C cytoplasms of several inbred lines of maize (Zea mays L.) and in other plant species.
- Published
- 1993
- Full Text
- View/download PDF