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Meta-analysis was used to compare yield protection and nematode suppression provided by two seed-applied and two soil-applied nematicides against Meloidogyne incognita and Rotylenchulus reniformis on cotton across 3 years and several trial locations in the U.S. Cotton Belt. Nematicides consisted of thiodicarb- and fluopyram-treated seed, aldicarb and fluopyram applied in furrow, and combinations of the seed treatments and soil-applied fluopyram. The nematicides had no effect on nematode reproduction or root infection but had a significant impact on seed cotton yield response ([Formula: see text]), with an average increase of 176 and 197 kg/ha relative to the nontreated control in M. incognita and R. reniformis infested fields, respectively. However, because of significant variation in yield protection and nematode suppression by nematicides, five or six moderator variables (cultivar resistance [M. incognita only], nematode infestation level, nematicide treatment, application method, trial location, and growing season) were used depending on nematode species. In M. incognita-infested fields, greater yield protection was observed with nematicides applied in furrow and with seed-applied + in-furrow than with solo seed-applied nematicide applications. Most notable of these in-furrow nematicides were aldicarb and fluopyram (>131 g/ha) with or without a seed-applied nematicide compared with thiodicarb. In R. reniformis-infested fields, moderator variables provided no further explanation of the variation in yield response produced by nematicides. Furthermore, moderator variables provided little explanation of the variation in nematode suppression by nematicides in M. incognita- and R. reniformis-infested fields. The limited explanation by the moderator variables on the field efficacy of nematicides in M. incognita- and R. reniformis-infested fields demonstrates the difficulty of managing these pathogens with nonfumigant nematicides across the U.S. Cotton Belt.

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Grape powdery mildew (GPM) fungicide programs consist of 5 to 15 applications, depending on region or market, in an attempt to achieve the high fruit quality standards demanded by the market. Understanding how fungicides redistribute and targeting redistributing fungicide to critical crop phenological stages could improve fungicide protection of grape clusters. This study evaluated fungicide redistribution in grapevines from major fungicide groups labeled for GPM control. Translaminar and xylem redistribution was examined by placing fungicide-impregnated filter disks on the adaxial or abaxial leaf surface of detached leaves for 10 min and then incubating for 48 h before inoculating the abaxial surface with conidia. Vapor redistribution used Teflon disks sprayed with fungicides and placed on the abaxial leaf surface of detached leaves 48 h before inoculation. Disease development was rated 10 days later. Translaminar movement through calyptra was tested using flowering potted vines. All fungicides tested redistributed through at least one mechanism. Fungicide timing at critical phenological stages (early, mid, and late bloom) was assessed in small plots of cultivar Pinot noir vines. The application of trifloxystrobin, quinoxyfen, or fluopyram at different bloom stages showed that applications initiated at end of bloom resulted in the lowest berry infection probabilities of 0.073, 0.097, and 0.020, respectively. The results of this study suggest that integrating two carefully timed applications of redistributing fungicides initiated at end of bloom into a fungicide program may be an effective strategy for wine grape growers in western Oregon to produce fruit with low GPM infection.

Meta-analysis was used to compare yield protection and nematode suppression provided by two seed-applied and two soil-applied nematicides against

In the Pacific Northwest, chasmothecia formation is not observed in vineyards until the beginning of véraison despite heavy infestations whereby 100% of leaf tissue is covered by Erysiphe necator. Mating type proximity and distribution were sampled from individual lesions (∼71 mm2) on leaf tissue in a stratified sampling from three canopy heights at three times during the 2013, 2014, and 2015 growing seasons. Both mating types were observed at every sampling point and within the same lesions at all sampling dates and canopy heights. Effect of temperature and day length were examined by inoculating seedlings with known mating type 1 and 2 isolates and placed in incubators at different temperatures (5, 10, 15, 20, 25, and 30°C) or different day length changes (long day to long day, long day to short day, short day to short day, and short day to long day). Chasmothecia were produced at all temperatures that E. necator was able to colonize tissue, and the greatest number of chasmothecia were produced at 15 and 20°C (P ≤ 0.02). Day length shifts from short day (8 h) to long day (16 h) resulted in a significant increase in chasmothecia production (P < 0.001). End of season plant stress observed in the Pacific Northwest, such as water stress or host senescence, was assessed under naturally infested field conditions by either girdling canes or applying 150 mg·liter−1 abscisic acid solution to vines, respectively, and quantifying chasmothecia production. No differences were observed in chasmothecia production in the plant stress assessment, likely due to the high vigor and ability for plants to overcome stress treatments.

In August 2018, sorghum plants (Sorghum bicolor (L.) Moench) from research field plots in Wake County, North Carolina were observed with head blight symptoms including panicles with red lesions, visible mycelium, and necrosis. At the time of collection, all plants in research plots displayed symptoms of Fusarium head blight and panicles averaged 33% area affected by symptoms and signs. From these affected plants, samples (n = 5) were collected for further identification. Symptomatic grains were surface sterilized for one minute in 0.825% sodium hypochlorite solution and rinsed for one minute in sterile, deionized water. After drying on sterile paper towels, grains were plated onto water agar. Resulting fungal hyphal tips were then transferred to antibiotic-amended potato dextrose agar (PDA) and incubated at 25oC. Cultures were incubated for 3 to 5 days. Isolates had abundant white hyphae accompanied with peach-colored pigment production. Macroconidia with 5-6 septations were 23.47 ± 7.74 µm long and 3.47 ± 0.66 µm wide with foot-shaped basal cells, tapering to hooked apical cells. Chlamydospores were present in chains but microconidia were not present. Morphological species recognition (MSR) criteria tentatively identified the isolate as Fusarium lacertarum Subrahm., in the Fusarium incarnatum-equiseti species complex (FIESC) using characteristics described by Leslie and Summerell 2006. Molecular characterization using translation elongation factor 1α (TEF-1 α, primers EF1 and EF2 from O'Donnell et al. 1998), β tubulin (TUB2, primers T1 and T22 from O'Donnell and Cigelnik 1997), and ribosomal protein subunit II (RPB2, primers 5F2 and 11AR from Ceron-Bustamante et al. 2018) was conducted to confirm morphological identification. DNA from the hyphae of pure cultures was extracted using the DNeasy PowerSoil DNA extraction kit according to manufacturer's guidelines. DNA amplification conditions followed the protocols for each primer set (O'Donnell et al. 1998; O'Donnell and Cigelnik 1997; Ceron-Bustamante et al. 2018). BLASTn analysis of TEF-1α (Isolate Accession MT149915, 573bp) alignment had 99.8% identity to F. lacertarum (NCBI accession: JF740828), TUB2 (Isolate Accession MT149914, 1,183bp) alignment had 99.3% identity to F. equiseti (NCBI accession: KJ396338), and RPB2 (Isolate Accession MT184173, 1,538bp) concatenated sequences had 95.3% identity to F. lacertarum (NCBI accession: MH582185). The TUB2 region most closely aligns to F. equiseti, which is likely due to an absence of TUB2 sequences labeled for F. lacertarum in the NCBI database. Pathogenicity was confirmed by spray-inoculating Southern Harvest 80G4 sorghum panicles (n = 9) at anthesis with four ml of conidial suspension (3.3×104 conidia/ml). Control plants (n = 9) were sprayed with sterile water. Plastic bags were placed around panicles for 24 hours to ensure moist conditions during the infection period. Plants were maintained in a greenhouse under a 12-hour light cycle and fertilized bi-weekly with 20-20-20 fertilizer. Symptoms were observed on inoculated panicles after 14 days, and the F. lacertarum isolate was recovered from inoculated plants and confirmed using methods described above. Fusarium spp. were not re-isolated from non-inoculated control plants. Members of FIESC are known to contribute to the Fusarium Head Blight disease complex and may be capable of producing mycotoxins associated with infections (Lincy et al. 2011; Marin et al. 2012; Moretti 2017); however, mycotoxin characterization in F. lacertarum has not been characterized. To our knowledge, this is the first report of F. lacertarum causing disease to sorghum in North Carolina and the United States. Fusarium lacertarum may cause impactful losses to sorghum producers due to direct yield and quality losses by the pathogen as well as the potential for mycotoxins to impact trade.

In the Pacific Northwest, chasmothecia formation is not observed in vineyards until the beginning of véraison despite heavy infestations whereby 100% of leaf tissue is covered by

Management of grape powdery mildew (Erysiphe necator) and other polycyclic diseases relies on numerous fungicide applications that follow calendar or model-based application intervals, both of which assume that inoculum is always present. Quantitative molecular assays have been previously developed to initiate fungicide applications, and could be used to optimize fungicide application intervals throughout the growing season based on inoculum concentration. Airborne inoculum samplers were placed at one research and six commercial vineyards in the Willamette Valley of Oregon. Fungicide applications in all plots were initiated at the first detection of E. necator inoculum, and all subsequent fungicide application intervals were made based the grower’ standard calendar program or based on inoculum concentration. In adjusted-interval plots, fungicides were applied at the shortest labeled application interval when >10 spores were detected and the longest labeled application interval when

Sixty percent of the $109 million processed red raspberry industry of the United States occurs in northern Washington State. In 2012, late-summer symptoms of vascular wilt and root disease were observed in many raspberry plantings. These symptoms were initially attributed to Verticillium dahliae. However, diagnostic tests for the pathogen were often contradictory and other soilborne pathogens (Phytophthora rubi and Pratylenchus penetrans) or Raspberry bushy dwarf virus (RBDV) might also have been involved. Therefore, a survey was conducted in 2013 and 2014 to (i) establish the incidence and soil population levels of V. dahliae in red raspberry production fields, (ii) compare among diagnostic methods and laboratories for detecting and quantifying V. dahliae from raspberry field soil, and (iii) assess which pathogens are associated with late-summer disease symptoms of raspberry. Plant and soil samples were collected from 51 disease sites and 20 healthy sites located in 24 production fields. Samples were analyzed for the presence and quantity of each pathogen using traditional plating and extraction methods (V. dahliae, P. rubi, and P. penetrans), quantitative polymerase chain reaction (qPCR) (V. dahliae and P. rubi), and enzyme-linked immunosorbent assay (RBDV). Results showed that V. dahliae was present in 88% of the production fields and that detection of the pathogen differed by method and by laboratory: qPCR detected V. dahliae in the soil from approximately three times as many sites (51 of 71 total sites) as by plating on NP10 semi-selective medium (15 of 71 total sites). Soil populations of V. dahliae were slightly greater at disease sites, but the pathogen was detected with similar frequency from healthy sites and it was rarely isolated from diseased plants (4%). P. rubi, P. penetrans, and RBDV were also common in production fields (79, 91, and 53% of fields, respectively). Both P. rubi (soil and root samples) and P. penetrans (root populations only), but not RBDV, were more frequently found at disease sites than healthy sites, and the amount of P. rubi detected by qPCR was greater from disease sites than healthy sites. In addition, P. rubi was isolated from 27% of the symptomatic plants located at disease sites. Regardless of detection method, V. dahliae, P. rubi, and P. penetrans, either with or without RBDV, were more likely to co-occur at disease sites (73%) than healthy sites (35%), suggesting that a soilborne disease complex is present in raspberry production fields. Results indicate that P. rubi is the primary pathogen most strongly associated with late-summer symptoms of disease, but root populations of P. penetrans and higher soil populations of V. dahliae may also be of concern. Therefore, disease control methods should focus on all three soilborne pathogens.

Predictive models have been developed in several major grape-growing regions to correlate environmental conditions to Erysiphe necator ascospore release; however, these models may not be broadly applicable in regions with different climatic conditions. To assess ascospore release in near-coastal regions of western Oregon, chasmothecia (syn. cleistothecia) were collected prior to leaf drop and placed onto natural and artificial grape trunk segments and overwintered outside. Ascospore release was monitored for three overwintering seasons using custom impaction spore traps from leaf drop (Biologische Bundesanstalt, Bundessortenamt und Chemische Industrie [BBCH] 97) until the onset of the disease epidemic in the following growing season. Airborne inoculum was concurrently monitored in a naturally infested research vineyard. Weather and ascospore release data were used to assess previously developed models and correlate environmental conditions to ascospore release. Ascospore release was predicted by all models prior to bud break (BBCH 08), and was observed from the first rain event following the start of inoculum monitoring until monitoring ceased. Previously developed models overpredicted ascospore release in the Willamette Valley and predicted exhaustion of inoculum prior to bud break. The magnitude of ascospore release could not be correlated to environmental conditions; thus, a binary ascospore release model was developed where release is a function of the collective occurrence of the following factors within a 24-h period: >6 h of cumulative leaf wetness during temperatures >4°C, precipitation >2.5 mm, and relative humidity >80%. The Oregon model was validated using field-collected ascospore datasets, and predicted ascospore release with 66% accuracy (P = 0.02). Extant methods for estimating ascospore release may not be sufficiently accurate to use as predictive models in wet, temperate climatic regions.