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1. Development of mPing‐based activation tags for crop insertional mutagenesis

Author

Alexander Johnson, Edward Mcassey, Stephanie Diaz, Jacob Reagin, Priscilla S. Redd, Daymond R. Parrilla, Hanh Nguyen, Adrian Stec, Lauren A. L. McDaniel, Thomas E. Clemente, Robert M. Stupar, Wayne A. Parrott, and C. Nathan Hancock

Subjects
activation tagging, gene discovery, soybean, transposable element, Botany, QK1-989
Abstract

Abstract Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to induce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing‐based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. Expression analysis of a line that contained a heritable insertion of the mmPing20F activation tag indicated that the activation tag induced overexpression of the nearby soybean genes. This represents a significant advance in gene discovery technology as activation tags have the potential to induce more phenotypes than the original mPing element, improving the overall effectiveness of the mutagenesis system.

Published
2021
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2. Development of mPing‐based activation tags for crop insertional mutagenesis

Author

Stephanie Diaz, Thomas E. Clemente, Adrian O. Stec, Daymond R. Parrilla, Edward V. McAssey, Priscilla S. Redd, C. Nathan Hancock, Jacob Reagin, Lauren A. L. McDaniel, Robert M. Stupar, Hanh Nguyen, Alexander Johnson, and Wayne A. Parrott

Subjects
0106 biological sciences, Transposable element, Transgene, Mutagenesis (molecular biology technique), Plant Science, Computational biology, Biology, 01 natural sciences, Biochemistry, Genetics and Molecular Biology (miscellaneous), Insertional mutagenesis, Transposition (music), 03 medical and health sciences, soybean, Gene, Ecology, Evolution, Behavior and Systematics, Transposase, 030304 developmental biology, 2. Zero hunger, 0303 health sciences, Ecology, activation tagging, Botany, food and beverages, gene discovery, transposable element, Phenotype, QK1-989, 010606 plant biology & botany
Abstract

Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to induce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing‐based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. Expression analysis of a line that contained a heritable insertion of the mmPing20F activation tag indicated that the activation tag induced overexpression of the nearby soybean genes. This represents a significant advance in gene discovery technology as activation tags have the potential to induce more phenotypes than the original mPing element, improving the overall effectiveness of the mutagenesis system.

Published
2021

3. Development of

Author

Alexander, Johnson, Edward, Mcassey, Stephanie, Diaz, Jacob, Reagin, Priscilla S, Redd, Daymond R, Parrilla, Hanh, Nguyen, Adrian, Stec, Lauren A L, McDaniel, Thomas E, Clemente, Robert M, Stupar, Wayne A, Parrott, and C Nathan, Hancock

Subjects
activation tagging, food and beverages, gene discovery, soybean, transposable element, Original Research
Abstract

Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to induce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing‐based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. Expression analysis of a line that contained a heritable insertion of the mmPing20F activation tag indicated that the activation tag induced overexpression of the nearby soybean genes. This represents a significant advance in gene discovery technology as activation tags have the potential to induce more phenotypes than the original mPing element, improving the overall effectiveness of the mutagenesis system.

Published
2020

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