1. Protection of transgenic tobacco plants expressing bovine pancreatic ribonuclease against tobacco mosaic virus.
- Author
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Trifonova EA, Sapotsky MV, Komarova ML, Scherban AB, Shumny VK, Polyakova AM, Lapshina LA, Kochetov AV, and Malinovsky VI
- Subjects
- Animals, Cattle, Plants, Genetically Modified, Nicotiana metabolism, Plant Diseases genetics, Plant Diseases virology, Ribonuclease, Pancreatic genetics, Ribonuclease, Pancreatic metabolism, Nicotiana genetics, Nicotiana virology, Tobacco Mosaic Virus physiology
- Abstract
Transgenic tobacco plants (Nicotiana tabacum cv. SR1) expressing extracellular pancreatic ribonuclease from Bos taurus and characterized by an increased level of ribonuclease activity in leaf extracts were challenged with tobacco mosaic virus. The transgenic plants exhibited a significantly higher level of protection against the virus infection than the control non-transformed plants. The protection was evidenced by the absence (or significant delay) of the appearance of typical mosaic symptoms and the retarded accumulation of infectious virus and viral antigen. These results demonstrate that modulation of extracellular nuclease expression can be efficiently used in promoting protection against viral diseases.
- Published
- 2007
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