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Among the 13 T1 plants analyzed, eight of them were quadruple mutants (Figure 1g), (ii) The gene-editing efficiency differed significantly among the four target genes (Figure 1g), (iii) I GENE1 i , I GENE2, i and I GENE3 i were not null in nine T1 plants of the TKC-M1 because they contained deletions of 3 or 9 bp. Editing gene families by CRISPR/Cas9: accelerating the isolation of multiple transgene-free null mutant combinations with much reduced labor-intensive analysis Keywords: CRISPR; transgene-free; multi; TKC; rice EN CRISPR transgene-free multi TKC rice 241 243 3 01/17/22 20220201 NES 220201 Genetically elucidating functions of gene families often requires simultaneous disruption of several family members to overcome redundancy. [Extracted from the article]