1. A histopathological classification system of Tyr::NRASQ61Kmurine melanocytic lesions: A reproducible simplified classification
- Author
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Léa Legrand, Pierre Sohier, Zackie Aktary, Christine Grill, Véronique Delmas, Lionel Larue, B. Vergier, Edouard Reyes-Gomez, Florence Bernex, Signalisation normale et pathologique de l'embryon aux thérapies innovante des cancers, Institut Curie-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Physiopathologie du cancer du foie, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de pathologie [Bordeaux], Université Bordeaux Segalen - Bordeaux 2-CHU Bordeaux [Bordeaux]-Groupe hospitalier Pellegrin, Institut de Recherche en Cancérologie de Montpellier (IRCM - U1194 Inserm - UM), CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), BioCampus Montpellier (BCM), Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Génétique fonctionnelle et médicale (GFM - ENVA), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), ANR-10-IDEX-0001-02/11-LABX-0038,CelTisPhyBio,Des cellules aux tissus: au croisement de la Physique et de la Biologie(2010), Signalisation normale et pathologique de l'embryon aux thérapies innovantes des cancers, Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National Du Cancer, Ligue Contre le Cancer - comite de l'Oise, ITMO Cancer, ANR Labex CelTisPhyBio [ANR-11-LBX-0038, ANR-10-IDEX-0001-02 PSL], INCa, and ANR-10-IDEX-0001,PSL,Paris Sciences et Lettres(2010)
- Subjects
0301 basic medicine ,melanocyte ,MESH: Melanoma ,MESH: Mice, Transgenic ,Computer science ,education ,melanoma model ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,MESH: Monomeric GTP-Binding Proteins ,Dermatology ,Computational biology ,transgenic mice ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Cohen's kappa ,melanoma ,MESH: Animals ,MESH: Mice ,MESH: Mutation, Missense ,β-catenin ,Mouse Melanoma ,Ink4a ,MESH: Amino Acid Substitution ,Pten ,030104 developmental biology ,Oncology ,Homogeneous ,030220 oncology & carcinogenesis ,Genetically Engineered Mouse ,histopathology ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,Ras - Abstract
International audience; Genetically engineered mouse models offer essential opportunities to investigate the mechanisms of initiation and progression in melanoma. Here, we report a new simplified histopathology classification of mouse melanocytic lesions in Tyr::NRASQ61K derived models, using an interactive decision tree that produces homogeneous categories. Reproducibility for this classification system was evaluated on a panel of representative cases of murine melanocytic lesions by pathologists and basic scientists. Reproducibility, measured as inter-rater agreement between evaluators using a modified Fleiss' kappa statistic, revealed a very good agreement between observers. Should this new simplified classification be adopted, it would create a robust system of communication between researchers in the field of mouse melanoma models.
- Published
- 2017
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