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3 results on '"Krenová D"'

1. Genetic isolation of quantitative trait loci for blood pressure development and renal mass on chromosome 5 in the spontaneously hypertensive rat

Author

Michal Pravenec, Kren V, Krenová D, Zídek V, Simáková M, Musilová A, Vorlícek J, Es, Lezin, and Tw, Kurtz

Subjects
Male, Analysis of Variance, Time Factors, Genotype, Body Weight, Quantitative Trait Loci, Chromosome Mapping, Blood Pressure, Hypertrophy, Organ Size, Kidney, Chromosomes, Mammalian, Rats, Animals, Congenic, Heart Rate, Rats, Inbred BN, Rats, Inbred SHR, Hypertension, Animals, Telemetry, Female, Crosses, Genetic
Abstract

Total genome scans of genetically segregating populations derived from spontaneously hypertensive rats (SHR) and other rat models of essential hypertension suggested a presence of quantitative trait loci (QTL) regulating blood pressure on multiple chromosomes, including chromosome 5. The objective of the current study was to test directly a hypothesis that chromosome 5 of the SHR carries a blood pressure regulatory QTL. A new congenic strain was derived by replacing a segment of chromosome 5 in the SHR/Ola between the D5Wox20 and D5Rat63 markers with the corresponding chromosome segment from the normotensive Brown Norway (BN/Crl) rat. Arterial pressures were directly monitored in conscious, unrestrained rats by radiotelemetry. The transfer of a segment of chromosome 5 from the BN strain onto the SHR genetic background was associated with a significant decrease of systolic blood pressure, that was accompanied by amelioration of renal hypertrophy. The heart rates were not significantly different in the SHR compared to SHR chromosome 5 congenic strain. The findings of the current study demonstrate that gene(s) with major effects on blood pressure and renal mass exist in the differential segment of chromosome 5 trapped within the new SHR.BN congenic strain.

Published
2003

2. Genetic analysis of "metabolic syndrome" in the spontaneously hypertensive rat.

Author

Pravenec M, Zídek V, Landa V, Simáková M, Mlejnek P, Kazdová L, Bílá V, Krenová D, and Kren V

Subjects
Animals, Animals, Genetically Modified, Chromosome Mapping, Gene Expression, Hemodynamics genetics, Rats, Rats, Inbred BN, Rats, Inbred SHR, Metabolic Syndrome genetics, Quantitative Trait Loci
Abstract

In the current review, we summarize results of genetic analyses of "metabolic syndrome" in the spontaneously hypertensive rat (SHR). These results include (1) linkage analyses in the HXB/BXH recombinant inbred (RI) strains derived from SHR and Brown Norway (BN-Lx) strains which revealed quantitative trait loci (QTL) for hemodynamic and metabolic traits on several chromosomes, (2) genetic isolation of these putative QTL within differential chromosome segments of SHR.BN congenic strains, (3) detailed mapping of these QTL within limited chromosome segments of SHR.BN congenic sublines, (4) sequencing of selected positional candidate genes which revealed important mutations in the Cd36 and Srebp1 SHR genes, (5) functional tests of these candidate genes in SHR transgenic lines, and (6) integrated gene expression profiling and linkage mapping in RI strains which will be used to identify co-regulated genes and to determine co-segregation of transcriptional profiles with physiological and pathophysiological phenotypes.

Published
2004

3. Genetic isolation of quantitative trait loci for blood pressure development and renal mass on chromosome 5 in the spontaneously hypertensive rat.

Author

Pravenec M, Kren V, Krenová D, Zídek V, Simáková M, Musilová A, Vorlícek J, Lezin ES, and Kurtz TW

Subjects
Analysis of Variance, Animals, Animals, Congenic, Blood Pressure genetics, Blood Pressure physiology, Body Weight, Chromosome Mapping, Crosses, Genetic, Female, Genotype, Heart Rate, Hypertension physiopathology, Hypertrophy pathology, Male, Organ Size, Rats, Rats, Inbred BN, Rats, Inbred SHR, Telemetry methods, Time Factors, Chromosomes, Mammalian genetics, Hypertension genetics, Kidney pathology, Quantitative Trait Loci genetics
Abstract

Total genome scans of genetically segregating populations derived from spontaneously hypertensive rats (SHR) and other rat models of essential hypertension suggested a presence of quantitative trait loci (QTL) regulating blood pressure on multiple chromosomes, including chromosome 5. The objective of the current study was to test directly a hypothesis that chromosome 5 of the SHR carries a blood pressure regulatory QTL. A new congenic strain was derived by replacing a segment of chromosome 5 in the SHR/Ola between the D5Wox20 and D5Rat63 markers with the corresponding chromosome segment from the normotensive Brown Norway (BN/Crl) rat. Arterial pressures were directly monitored in conscious, unrestrained rats by radiotelemetry. The transfer of a segment of chromosome 5 from the BN strain onto the SHR genetic background was associated with a significant decrease of systolic blood pressure, that was accompanied by amelioration of renal hypertrophy. The heart rates were not significantly different in the SHR compared to SHR chromosome 5 congenic strain. The findings of the current study demonstrate that gene(s) with major effects on blood pressure and renal mass exist in the differential segment of chromosome 5 trapped within the new SHR.BN congenic strain.

Published
2003

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