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Start Over Descriptor "Estrogen signaling pathway" Journal physiological reports
2 results on '"Estrogen signaling pathway"'

1. A transcriptomics model of estrogen action in the ovine fetal hypothalamus: evidence for estrogenic effects of ICI 182,780.

Author

Rabaglino, Maria Belen, Keller‐Wood, Maureen, and Wood, Charles E.

Subjects
*TRANSCRIPTOMES, *HYPOTHALAMUS, *NEURAL development, *HYPOTHALAMIC-pituitary-adrenal axis, *ESTROGEN receptors
Abstract

Abstract: Estradiol plays a critical role in stimulating the fetal hypothalamus–pituitary–adrenal axis at the end of gestation. Estradiol action is mediated through nuclear and membrane receptors that can be modulated by ICI 182,780, a pure antiestrogen compound. The objective of this study was to evaluate the transcriptomic profile of estradiol and ICI 182,780, testing the hypothesis that ICI 182,780 antagonizes the action of estradiol in the fetal hypothalamus. Chronically catheterized ovine fetuses were infused for 48 h with: vehicle (Control, n = 6), 17β‐estradiol 500 μg/kg/day (Estradiol, n = 4), ICI 182,780 5 μg/kg/day (ICI 5 μg, n = 4) and ICI 182,780 5 mg/kg/day (ICI 5 mg, n = 5). Fetal hypothalami were collected afterward, and gene expression was measured through microarray. Statistical analysis of transcriptomic data was performed with Bioconductor‐R and Cytoscape software. Unexpectedly, 35% and 15.5% of the upregulated differentially expressed genes (DEG) by Estradiol significantly overlapped (P < 0.05) with upregulated DEG by ICI 5 mg and ICI 5 μg, respectively. For the downregulated DEG, these percentages were 29.9% and 15.5%, respectively. There was almost no overlap for DEG following opposite directions between Estradiol and ICI ICI 5 mg or ICI 5 μg. Furthermore, most of the genes in the estrogen signaling pathway – after activation of the epidermal growth factor receptor – followed the same direction in Estradiol, ICI 5 μg or ICI 5 mg compared to Control. In conclusion, estradiol and ICI 182,780 have estrogenic genomic effects in the developing brain, suggesting the possibility that the major action of estradiol on the fetal hypothalamus involves another receptor system rather than estrogen receptors. [ABSTRACT FROM AUTHOR]

Published
2018
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2. A transcriptomics model of estrogen action in the ovine fetal hypothalamus: evidence for estrogenic effects of ICI 182,780

Author

Maria Belen Rabaglino, Maureen Keller-Wood, and Charles E. Wood

Subjects
0301 basic medicine, medicine.medical_specialty, Physiology, medicine.drug_class, Hypothalamus, Estrogen receptor, purl.org/becyt/ford/1 [https], Ciencias Biológicas, 03 medical and health sciences, 0302 clinical medicine, Fetus, MICROARRAY, Cell surface receptor, Pregnancy, Physiology (medical), Internal medicine, Gene expression, ESTROGEN SIGNALING PATHWAY, polycyclic compounds, medicine, Animals, Gene Regulatory Networks, Receptor, purl.org/becyt/ford/1.6 [https], Infusions, Intravenous, BRAIN DEVELOPMENT, Fulvestrant, Sheep, Chemistry, Biología del Desarrollo, FETAL PROGRAMMING, Estrogens, Antiestrogen, 030104 developmental biology, Endocrinology, Estrogen, Female, Estrogen Receptor Antagonists, Transcriptome, 030217 neurology & neurosurgery, hormones, hormone substitutes, and hormone antagonists, CIENCIAS NATURALES Y EXACTAS
Abstract

Estradiol plays a critical role in stimulating the fetal hypothalamus?pituitary?adrenal axis at the end of gestation. Estradiol action is mediated through nuclear and membrane receptors that can be modulated by ICI 182,780, a pure antiestrogen compound. The objective of this study was to evaluate the transcriptomic profile of estradiol and ICI 182,780, testing the hypothesis that ICI 182,780 antagonizes the action of estradiol in the fetal hypothalamus. Chronically catheterized ovine fetuses were infused for 48 h with: vehicle (Control, n = 6), 17β‐estradiol 500 μg/kg/day (Estradiol, n = 4), ICI 182,780 5 μg/kg/day (ICI 5 μg, n = 4) and ICI 182,780 5 mg/kg/day (ICI 5 mg, n = 5). Fetal hypothalami were collected afterward, and gene expression was measured through microarray. Statistical analysis of transcriptomic data was performed with Bioconductor‐R and Cytoscape software. Unexpectedly, 35% and 15.5% of the upregulated differentially expressed genes (DEG) by Estradiol significantly overlapped (P < 0.05) with upregulated DEG by ICI 5 mg and ICI 5 μg, respectively. For the downregulated DEG, these percentages were 29.9% and 15.5%, respectively. There was almost no overlap for DEG following opposite directions between Estradiol and ICI ICI 5 mg or ICI 5 μg. Furthermore, most of the genes in the estrogen signaling pathway after activation of the epidermal growth factor receptor followed the same direction in Estradiol, ICI 5 μg or ICI 5 mg compared to Control. In conclusion, estradiol and ICI 182,780 have estrogenic genomic effects in the developing brain, suggesting the possibility that the major action of estradiol on the fetal hypothalamus involves another receptor system rather than estrogen receptors. Fil: Rabaglino, Maria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina Fil: Keller Wood, Maureen. University of Florida; Estados Unidos Fil: Wood, Charles E.. University of Florida; Estados Unidos

Published
2018

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