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The effects of foliar application of potassium 3,4‐dichloroisothiazole‐5‐carboxylate (TD‐1123) and S,S,S‐tributyl phosphorotrithioate (DEF) on some physiological events that occur in cotton (Gossypium hirsutum L.) leaves are reported. Transport of 14C from cotton leaves was significantly reduced as soon as 1 day after treatment with DEF. In addition the DEF‐treated leaves exhibited reductions in water potential recovery values (predawn measurements), stomatal conductance, specific weights, soluble N content, and an alteration in free amino acid distribution. Little change in any of these parameters was noted in leaves treated with TD‐1123 alone. A sequential treatment of TD‐1123 followed in 10 days with DEF had an apparent synergistic effect in that the effect on most parameters measured was more pronounced and occurred earlier in such leaves than in those treated with either chemical alone. [ABSTRACT FROM AUTHOR]