1. Unzipping and shearing DNA with electrophoresed nanoparticles in hydrogels
- Author
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Shin Ichiro M. Nomura, Keitel Cervantes-Salguero, Ibuki Kawamata, and Satoshi Murata
- Subjects
Electrophoresis ,Dna duplex ,Materials science ,Base pair ,Acrylic Resins ,General Physics and Astronomy ,Nanoparticle ,Metal Nanoparticles ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,chemistry.chemical_compound ,A-DNA ,Physical and Theoretical Chemistry ,Composite material ,Nucleic Acid Hybridization ,Hydrogels ,DNA ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,chemistry ,Colloidal gold ,Duplex (building) ,Self-healing hydrogels ,Gold ,0210 nano-technology - Abstract
We show electric control of unzipping and shearing dehybridization of a DNA duplex anchored to a hydrogel. Tensile force is applied by electrophoresing (25 V cm−1) gold nanoparticles pulling the DNA duplex. The pulled DNA strand is gradually released from the hydrogel. The unzipping release rate is faster than shearing; for example, 3-fold for a 15 base pair duplex, which helps to design electrically driven DNA devices.
- Published
- 2017