28 results on '"Tempestini, A"'
Search Results
2. Erythrosine as a photosensitizer for antimicrobial photodynamic therapy with blue light-emitting diodes – An in vitro study
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Gonçalves, Marcela Leticia Leal, Santos, Elaine Marcílio, Renno, Ana Cláudia Muniz, Horliana, Anna Carolina Ratto Tempestini, Cruz, Matheus de Almeida, Parisi, Julia Risso, Prates, Renato Araújo, Leal-Rossi, Adriana, Fernandes, Kristianne Porta Santos, Mesquita-Ferrari, Raquel Agnelli, and Bussadori, Sandra Kalil
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- 2021
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3. Clinical evaluation of in-office tooth whitening with violet LED (405 nm): A double-blind randomized controlled clinical trial
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Santos, Ana Eliza Castanho Garrini, Bussadori, Sandra Kalil, Pinto, Marcelo Mendes, Brugnera, Aldo Jr, Zanin, Fátima Antonia Aparecida, Silva, Tamiris, Martinbianco, Ana Luiza Cabrera, Pantano Junior, Dacio Antônio, Rodrigues, Maria Fernanda Setúbal Dextro, Artese, Hilana Paula Carrillo, Deana, Alessandro Melo, Motta, Lara Jansiski, and Horliana, Anna Carolina Ratto Tempestini
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- 2021
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4. Pericoronitis treatment with antimicrobial photodynamic therapy using a new formula of methylene blue: A case report
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Schalch, Tânia Oppido, primary, Piatto, Érika Thaís, additional, Simão, Débora de Sousa, additional, Bussadori, Sandra Kalil, additional, Motta, Lara Jansiski, additional, Pavani, Christiane, additional, and Horliana, Anna Carolina Ratto Tempestini, additional
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- 2023
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5. Pericoronitis treatment with antimicrobial photodynamic therapy using a new formula of methylene blue: A case report
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Tânia Oppido Schalch, Érika Thaís Piatto, Débora de Sousa Simão, Sandra Kalil Bussadori, Lara Jansiski Motta, Christiane Pavani, and Anna Carolina Ratto Tempestini Horliana
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Oncology ,Biophysics ,Pharmacology (medical) ,Dermatology - Published
- 2023
6. Effectiveness of Violet LED alone or in association with bleaching gel during dental photobleaching: A Systematic Review
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Bianca Rossi, Susana Morimoto, Tamara Kerber Tedesco, Sandra Ribeiro Cunha, Anna Carolina Ratto Tempestini Horliana, and Karen Müller Ramalho
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Photobleaching ,Oncology ,Photochemotherapy ,Biophysics ,Pharmacology (medical) ,Dermatology ,Hydrogen Peroxide ,Tooth Bleaching Agents ,Hypochlorous Acid ,Peroxides - Abstract
To conduct a systematic review to determine the efficacy of violet led in promoting dental bleaching by itself or accelerating dental bleaching when associated with peroxides.Clinical and in vitro studies were identified by a search on November 27th 2020 in the PubMed and Scopus databases. Inclusion criteria were: 1) studies related to bleaching; 2) studies related to violet LED Light (405-410nm); and 3) studies that analyzed efficacy. The authors assessed the studies for risk of bias independently. Authors extracted outcomes including color change evaluation and pain assessment independently.During the search process, 895 articles were found in the previously cited databases. After the first screening consisting of title and abstract evaluations, 18 articles were selected. Finally, 13 articles met the eligibility criteria and were included in this review, being 5 clinical trial/case series and 8 in vitro studies. In vitro studies showed a high risk of bias and interventional studies showed a low risk of bias.The violet Led seems to have the potential to bleach teeth without peroxides, with a clinical perceptible color alteration. However, the effect is small in comparison to bleaching using peroxides. When Violet Led is used in association with peroxides, it seems to potentialize the bleaching result. However, due to the high heterogeneity between studies, a small number of clinical studies, and the high risk of bias of the in vitro included studies, the results are not definitive, and further well-designed studies are needed to reach safe evidence.
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- 2022
7. Erythrosine as a photosensitizer for antimicrobial photodynamic therapy with blue light-emitting diodes - An in vitro study
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Adriana Leal-Rossi, Anna Carolina Ratto Tempestini Horliana, Sandra Kalil Bussadori, Kristianne Porta Santos Fernandes, Ana Claudia Muniz Renno, Renato Araujo Prates, Marcela Leticia Leal Gonçalves, Raquel Agnelli Mesquita-Ferrari, Julia Risso Parisi, Elaine Marcílio Santos, and Matheus de Almeida Cruz
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medicine.medical_treatment ,Biophysics ,Photodynamic therapy ,Dermatology ,Absorbance ,chemistry.chemical_compound ,Anti-Infective Agents ,Candida albicans ,medicine ,Pharmacology (medical) ,Photosensitizer ,Viability assay ,Irradiation ,Photosensitizing Agents ,biology ,biology.organism_classification ,Erythrosine ,Corpus albicans ,Methylene Blue ,Oncology ,chemistry ,Photochemotherapy ,Biofilms ,Nuclear chemistry - Abstract
Background This study aims to test the absorbance of a new composition of erythrosine, its pH, cell viability and potential as a photo sensitizer against Candida albicans when irratiaded with blue light emitting-diode (LED). Methods For pH and absorbance tests, erythrosine was prepared at a concentration of 0.03/ml. The cells of the L929 strain were cultured and the alamarBlue® assay was performed on samples to assess cell viability. For the microbiological essay, the strain of Candida albicans ATCC 90028 was selected. Yeast suspensions were divided into the following groups: control without irradiation or photosensitizer (C), irradiated group without photosensitizer (L), photosensitizer group without irradiation (0), and groups that received photosensitizer and irradiation, called aPDT groups. Results Erythrosine had no significant changes in pH and its absorbance was also consistent (≅400 nm). When it came to cell viability, on the first day, the group that was in contact with the dye and irradiated with the LED in minimun power was found to have the higher cell proliferation. On day 3, both irradiated groups (maximum and minimum) showed the highest cell proliferation. In the microbiological essay with C. albicans, aPDT groups started to show microbial reduction after 60 and 90 s of irradiation and when irradiated for 120 s, 6 microbial reduction logs were found. Conclusions The erythrosine in question is a PS, with pH stability, blue light absorbance, cell viability and efficacy against C. albicans. More studies with this PS should be encouraged in order to verify its performance in aPDT.
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- 2021
8. Parameters for antimicrobial photodynamic therapy on periodontal pocket—Randomized clinical trial
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Christiane Pavani, Anna Carolina Ratto Tempestini Horliana, Martha S. Ribeiro, Giovane Noschese, Sandra Kalil Bussadori, Daniela de Fátima Teixeira da Silva, Ilka Tiemy Kato, Letícia Heineck Alvarenga, Renato Araujo Prates, Mark Wainwright, Yhago Gazote Eloy Geraldo, Bianca Godoy-Miranda, Ana Carolina Gomes, and Pamela Carribeiro
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Male ,RM ,Time Factors ,Gingival and periodontal pocket ,Drug Compounding ,medicine.medical_treatment ,030303 biophysics ,Biophysics ,Dentistry ,Photodynamic therapy ,Dermatology ,law.invention ,Surface-Active Agents ,030207 dermatology & venereal diseases ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Randomized controlled trial ,law ,medicine ,Humans ,Periodontal Pocket ,Pharmacology (medical) ,Photosensitizer ,0303 health sciences ,Photosensitizing Agents ,business.industry ,Antimicrobial ,medicine.disease ,Combined Modality Therapy ,Chronic periodontitis ,QR ,Methylene Blue ,Clinical trial ,Photochemotherapy ,Oncology ,chemistry ,Female ,Periodontal Index ,business ,Methylene blue - Abstract
BACKGROUND: Antimicrobial photodynamic therapy (aPDT) has been investigated as an adjunctive to periodontal treatment but the dosimetry parameters adopted have discrepancies and represent a challenge to measure efficacy. There is a need to understand the clinical parameters required to obtain antimicrobial effects by using aPDT in periodontal pockets. The aim of this study was to investigate parameters relating to the antimicrobial effects of photodynamic therapy in periodontal pockets. MATERIAL AND METHODS: This randomized controlled clinical trial included 30 patients with chronic periodontitis. Three incisors from each patient were selected and randomized for the experimental procedures. Microbiological evaluations were performed to quantify microorganisms before and after treatments and spectroscopy was used to identify methylene blue in the pocket. A laser source with emission of radiation at wavelength of ʎ = 660 nm and output radiant power of 100 mW was used for 1, 3 and 5 min. One hundred μM methylene blue was used in aqueous solution and on surfactant vehicle. RESULTS: The results demonstrated the absence of any antimicrobial effect with aqueous methylene blue-mediated PDT. On the other hand, methylene blue in the surfactant vehicle produced microbial reduction in the group irradiated for 5 min (p
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- 2019
9. Use of optical fluorescence for the diagnosis of dental biofilm in young permanent molars - A case series
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Cardoso, Aretusa, primary, Fernandes, Juliana Terra, additional, Bussadori, Sandra Kalil, additional, Horliana, Anna Carolina Ratto Tempestini, additional, Fernandes, Kristianne Porta Santos, additional, Gonçalves, Marcela Leticia Leal, additional, and Motta, Lara Jansiski, additional
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- 2021
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10. Oral hygiene associated with antimicrobial photodynamic therapy or lingual scraper in the reduction of halitosis after 90 days follow up: A randomized, controlled, single-blinded trial
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Romero, Sergio Santos, primary, do Vale, Katia Llanos, additional, Remolina, Vanessa Gomes, additional, Silva, Thayná Gomes, additional, Schalch, Tânia Oppido, additional, Ramalho, Karen Muller, additional, Negreiros, Renata Matalon, additional, Ando, Ellen Sayuri, additional, Mayer, Marcia Pinto Alves, additional, Mesquita Ferrari, Raquel Agnelli, additional, Motta, Lara Jansiski, additional, Fernandes, Kristianne Porta Santos, additional, Bussadori, Sandra Kalil, additional, and Horliana, Anna Carolina Ratto Tempestini, additional
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- 2021
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11. Treatment of halitosis with photodynamic therapy in older adults with complete dentures: A randomized, controlled, clinical trial
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Llanos do Vale, Katia, primary, Ratto Tempestini Horliana, Anna Carolina, additional, Romero dos Santos, Sergio, additional, Oppido Schalch, Tania, additional, Melo de Ana, Alessandro, additional, Agnelli Mesquita Ferrari, Raquel, additional, Kalil Bussadori, Sandra, additional, and Porta Santos Fernandes, Kristianne, additional
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- 2021
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12. Photodynamic therapy and Acyclovir in the treatment of recurrent herpes labialis: A controlled randomized clinical trial
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Ramalho, Karen Müller, primary, Cunha, Sandra Ribeiro, additional, Gonçalves, Flavia, additional, Escudeiro, Gustavo Silva, additional, Steiner-Oliveira, Carolina, additional, Horliana, Anna Carolina Rato Tempestini, additional, and Eduardo, Carlos de Paula, additional
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- 2021
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13. Photodynamic therapy and Acyclovir in the treatment of recurrent herpes labialis: A controlled randomized clinical trial
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Anna Carolina Rato Tempestini Horliana, Sandra Ribeiro Cunha, Flávia Gonçalves, Carlos de Paula Eduardo, Karen Müller Ramalho, Gustavo Silva Escudeiro, and Carolina Steiner-Oliveira
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medicine.medical_specialty ,medicine.medical_treatment ,030303 biophysics ,Biophysics ,Statistical difference ,Acyclovir ,Photodynamic therapy ,Dermatology ,Herpesvirus 1, Human ,medicine.disease_cause ,law.invention ,Lesion ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,law ,Recurrence ,Edema ,medicine ,Humans ,Pharmacology (medical) ,0303 health sciences ,Photosensitizing Agents ,business.industry ,Significant difference ,Herpes simplex virus ,Oncology ,Photochemotherapy ,Recurrent herpes labialis ,medicine.symptom ,Herpes Labialis ,business - Abstract
Background Herpes Simplex Virus Type 1 (HSV-1) is one of the most widespread infections that can effect the orofacial region. Recurrent infection is considered a life-long oral health problem, leading to pain, discomfort, and social restriction due to esthetic features when active. Effective therapies are needed. This study aimed to compare photodynamic therapy (PDT), Topical Acyclovir (AC), and the association of both in the healing process and self-reported symptomologies of HSV-1 recurrences. Methods Patients were randomly assigned into 3 groups (n = 25): PDT (low-power laser, 660 nm, 40 mW, 120 J/cm2, 4.8 J, 120 s per point) and methylene blue (0.005 %) as photosensitizer; AC (5%); PDT + AC.Data concerning lesion size, healing time, and self-reported healing parameters, such as pain, tingling, and edema were taken every day up to complete healing for all studied groups. Results There was no significant difference in healing time and pain between groups. AC group showed a significant minor reduction of the lesion compared to the AC-PDT group on day 1. Regarding edema and tingling, the comparison of treatments showed a statistical difference only on day 1, where PDT showed better results. Conclusion With all the limitations of this study, it can be concluded that only on day 1 PDT showed positive effects in the treatment of herpes lesions in comparison to AC.
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- 2020
14. Clinical evaluation of in-office tooth whitening with violet LED (405 nm): A double-blind randomized controlled clinical trial
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Hilana Paula Carrillo Artese, Lara Jansiski Motta, Maria Fernanda Setúbal Dextro Rodrigues, Tamiris Silva, Alessandro Melo Deana, Ana Eliza Castanho Garrini dos Santos, Anna Carolina Ratto Tempestini Horliana, Ana Luiza Cabrera Martinbianco, Fatima A. A. Zanin, Dacio Antônio Pantano Junior, Aldo Brugnera, Marcelo Mendes Pinto, and Sandra Kalil Bussadori
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Visual analogue scale ,030303 biophysics ,Biophysics ,Dentistry ,Dermatology ,Double blind ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Tooth Bleaching ,Humans ,Medicine ,Pharmacology (medical) ,Tooth Bleaching Agents ,Tooth whitening ,0303 health sciences ,Photosensitizing Agents ,business.industry ,Clinical trial ,Photochemotherapy ,Oncology ,Tooth Sensitivity ,Quality of Life ,Violet light ,Carbamide peroxide ,business ,Clinical evaluation - Abstract
Although there is little evidence showing the effectiveness of violet LED on in-office tooth whitening, there are some studies which have reported satisfactory results. This double-blind randomized controlled clinical trial aimed to evaluate the effect of a violet light emission diode (LED) (405 nm) system, used in-office, on tooth whitening, sensitivity, use of medication after whitening and quality of life.Eight patients were randomized into 4 groups (n = 20): G1 - violet LED, G2 - 35% carbamide peroxide (CP) and violet LED, G3 only CP 35% and G4 35% hydrogen peroxide (HP). The color was measured using a spectrophotometer at the following times: baseline, 15 and 180days. The colorimetric changes were analyzed using measurements from the CieLab System ΔE (ΔL, Δa and Δb) and the WID (Whiteness Index for Dentistry) for the 4 groups. Tooth sensitivity was recorded via a visual analog scale (VAS). Additionally, the Psychosocial Impact of Dental. Aesthetics Questionnaire (PIDAQ) was used to evaluate quality of life.For the analysis of ΔL, G4 and G2 presented more brightness than G1 and G3 (p0.05), implying that G2 is at least as good as G4 (p0.05). For measure of Δa, G4 presented better results, standing out from the other groups. (p0.05). For Δb, both G4 and G2 showed a greater tendency for blue color than groups G1 and G3 (p0.05). When analyzing ∆E at the 180-day follow up, G4 produced the highest ∆E, while G3 showed the lowest ∆E. The other 2 whitening groups produced intermediate ∆E values. For ∆W, G1 and G3 significantly differed from G2 and G3. When analyzing the 180-day follow up, G2 produced the highest ∆W, while G3 showed the lowest ∆W. The other two whitening treatments produced intermediate ∆W values. As for sensitivity, only G4 patients showed dental sensitivity within 24 h of the bleaching, with pain ceasing after 48 h. For G4, 33% of the patients needed to take analgesics within the first 24 h after the first 3 whitening sessions. For PIDAQ, there was no overall decrease in score over time for any of the groups and there was no difference between them (p0.05).Our results showed that violet LED was not able to whiten teeth at the same intensity, when used alone, as it was when associated with 35% CP, contrary to our initial hypothesis. However, given that pain was not consistently reported in G2, one could suggest that treatment with LED + 35% CP is quite similar to that of 35% HP when used for tooth whitening, but with better pain outcomes. All treatments suggested an improvement in quality of life.Clinical Trial Registry: This trial is registered at ClinicalTrials.gov; the registration number is NCT03192852 https://clinicaltrials.gov/ct2/show/NCT03192852.
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- 2021
15. Photodynamic therapy for endodontic treatment of primary teeth: A randomized controlled clinical trial
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Okamoto, Camila Basilio, primary, Bussadori, Sandra Kalil, additional, Prates, Renato Araujo, additional, da Mota, Ana Carolina Costa, additional, Tempestini Horliana, Anna Carolina Ratto, additional, Fernandes, Kristianne Porta Santos, additional, and Motta, Lara Jansiski, additional
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- 2020
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16. Antimicrobial photodynamic therapy with Bixa orellana extract and blue LED in the reduction of halitosis—A randomized, controlled clinical trial
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Gonçalves, Marcela Leticia Leal, primary, da Mota, Ana Carolina Costa, additional, Deana, Alessandro Melo, additional, Cavalcante, Lisyanne Araújo de Souza, additional, Horliana, Anna Carolina Ratto Tempestini, additional, Pavani, Christiane, additional, Motta, Lara Jansiski, additional, Fernandes, Kristianne Porta Santos, additional, Mesquita-Ferrari, Raquel Agnelli, additional, da Silva, Daniela Fátima Teixeira, additional, Motta, Pamella de Barros, additional, Araújo Prates, Renato, additional, and Bussadori, Sandra Kalil, additional
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- 2020
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17. Preventive effect of remineralizing materials on dental erosion lesions by speckle technique: An in vitro analysis
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Olivan, Silvia Regina Garcia, primary, Sfalcin, Ravana Angelini, additional, Fernandes, Kristianne Porta Santos, additional, Ferrari, Raquel Agnelli Mesquita, additional, Horliana, Anna Carolina Ratto Tempestini, additional, Motta, Lara Jansiski, additional, Ortega, Silvana Machado, additional, Pinto, Marcelo Mendes, additional, Deana, Alessandro Melo, additional, and Bussadori, Sandra Kalil, additional
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- 2020
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18. Photodynamic antimicrobial chemotherapy action of phenothiazinium dyes in planktonic Candida albicans is increased in sodium dodecyl sulfate
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Alves da Collina, Gabriela, primary, Freire, Fernanda, additional, da Silva Barbosa, Vinicius, additional, Bento Correa, Caroline, additional, Reis Nascimento, Helenyce, additional, Ratto Tempestini Horliana, Anna Carolina, additional, Teixeira da Silva, Daniela de Fátima, additional, Araujo Prates, Renato, additional, and Pavani, Christiane, additional
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- 2020
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19. “Efficacy of photodynamic therapy on the treatment of herpes labialis: A systematic review”
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Lotufo, Monica Andrade, primary, Tempestini Horliana, Anna Carolina Ratto, additional, Santana, Thalita, additional, de Queiroz, Aline Campos, additional, Gomes, Andréa Oliver, additional, Motta, Lara Jansiski, additional, Ferrari, Raquel Agnelli Mesquita, additional, dos Santos Fernandes, Kristianne Porta, additional, and Bussadori, Sandra Kalil, additional
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- 2020
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20. Use of optical fluorescence for the diagnosis of dental biofilm in young permanent molars - A case series
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Sandra Kalil Bussadori, Anna Carolina Ratto Tempestini Horliana, Marcela Leticia Leal Gonçalves, Juliana Terra Fernandes, Aretusa Cardoso, Lara Jansiski Motta, and Kristianne Porta Santos Fernandes
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Molar ,Diagnostic methods ,030303 biophysics ,Combined use ,Biophysics ,Dentistry ,Dermatology ,Oral Hygiene Index ,Fluorescence ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Humans ,Medicine ,Pharmacology (medical) ,Child ,0303 health sciences ,Photosensitizing Agents ,business.industry ,Significant difference ,Biofilm ,Repeated measures design ,Optical fluorescence ,Photochemotherapy ,Oncology ,Biofilms ,business ,Brazil - Abstract
Background Fluorescence appears clearly in oral biofilm in red tones, showing the presence of microorganisms in regions where there is biofilm accumulation. This study aims to evaluate the applicability and effectiveness of the diagnosis of oral biofilm with the optical fluorescence technique using the EVINCE (Evidenciador Clinico – MMOptics, Sao Carlos, SP, Brazil) equipment. Furthermore, to compare the efficacy of the optical fluorescence diagnostic method with the traditional method of clinical disclosure of Fuchsin-based dye biofilm and to observe their combined use. Methods Sixteen children, aged between 7 and 12, were included in this case series, following the Oral Hygiene Index – Simplificated (OHI-S) evaluation. They were evaluated by 3 different professionals. The 1 st evaluator checked the OHI-S observing only with EVINCE. In the second stage, a 2nd evaluator performed the traditional disclosure technique with Fucsina, and finally a 3rd evaluator who observed with EVINCE the teeth previously stained in stage 2, combining the two methods. Descriptive analysis of the variables was performed and comparative tests of repeated measures to evaluate differences between the results of the three evaluation methodologies. Results There is no significant difference between the observation made only with EVINCE and with the traditional methodology of plaque disclosure. However, there is a difference when the two techniques are used in the third evaluation moment, showing that the combination could provide better results. Conclusions The association of both the conventional method and the use of EVINCE showed a very satisfactory result for the diagnosis of the presence of biofilm.
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- 2021
21. Preventive effect of remineralizing materials on dental erosion lesions by speckle technique: An in vitro analysis
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Kristianne Porta Santos Fernandes, Silvia Regina Garcia Olivan, Lara Jansiski Motta, Raquel Agnelli Mesquita Ferrari, Silvana Machado Ortega, Alessandro Melo Deana, Anna Carolina Ratto Tempestini Horliana, Ravana Angelini Sfalcin, Sandra Kalil Bussadori, and Marcelo Mendes Pinto
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business.product_category ,Surface Properties ,GC America ,Biophysics ,Dentistry ,Dermatology ,Diamines ,In Vitro Techniques ,In vitro analysis ,Fluorides ,Materials Testing ,Medicine ,In vitro study ,Animals ,Pharmacology (medical) ,Fluorides, Topical ,Tooth Erosion ,Dental Enamel ,Toothpaste ,Enamel paint ,business.industry ,Fluoride varnish ,Significant difference ,Optical Imaging ,Cariostatic Agents ,Oncology ,visual_art ,visual_art.visual_art_medium ,Sodium Fluoride ,Cattle ,Soft drink ,business ,Toothpastes - Abstract
The aim of this in vitro study was to evaluate the preventive effect of different materials on dental erosion lesions by speckle coherent light scattering analysis. Forty bovine teeth were divided in the following groups (n = 10): 1) DURA- Preventive Treatment with Fluoride Varnish (Duraphat, Colgate-Palmolive); 2) ELX- Preventive treatment with Elmex® Erosion Protection Toothpaste (GABA International AG, Therwil, Switzerland); 3) MP- Preventive treatment with MI Paste® (GC America); and 4) REG- Preventive Treatment with Regenerate Enamel Science™ (Unilever). For all groups, each sample was divided into 3 areas: non-treatment (control); preventive treatment + erosive challenge; non-treatment + erosive challenge. The erosive challenge was carried out using Sprite® Zero soft drink (pH 2.58). After the erosive challenge, the samples were evaluated by speckle coherent light scattering method in the eroded area compared to the sound area. The results showed that there was a statistically significant difference between eroded area with and without preventive treatment, however, there was no statistically significant difference among the different preventive materials tested.
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- 2019
22. Photodynamic antimicrobial chemotherapy action of phenothiazinium dyes in planktonic Candida albicans is increased in sodium dodecyl sulfate
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Anna Carolina Ratto Tempestini Horliana, Fernanda Freire, Helenyce Reis do Nascimento, Christiane Pavani, Caroline Bento Correa, Gabriela Alves da Collina, Daniela de Fátima Teixeira da Silva, Renato Araujo Prates, and Vinicius da Silva Barbosa
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food.ingredient ,030303 biophysics ,Biophysics ,Azure A ,Dermatology ,Pact ,030207 dermatology & venereal diseases ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,food ,Anti-Infective Agents ,Candida albicans ,Agar ,Pharmacology (medical) ,Sodium dodecyl sulfate ,Coloring Agents ,0303 health sciences ,Photosensitizing Agents ,biology ,Sodium Dodecyl Sulfate ,Antimicrobial ,biology.organism_classification ,Plankton ,Oncology ,chemistry ,Photochemotherapy ,Urea ,Methylene blue ,Nuclear chemistry - Abstract
Background Photodynamic antimicrobial chemotherapy (PACT) is an important therapeutic platform for antimicrobial activity, especially due to the resistant strains, however, the physical-chemical properties of the photosensitizers may affect the final outcome. Recently, this research group showed that the control of aggregation potentiates the PACT action of Methylene Blue. In this study, the researchers investigated the PACT action of other phenothiazinium dyes (PDs), such as Azure A, Azure B (AB), and 1,9-dimethyl methylene blue (DMMB), and the effects of different mediums which modulate PD aggregation were studied. Methods The C. albicans planktonic culture was treated with PDs at different concentrations (0–100.00 mg/L), both in the dark (5 min) and irradiated (640 ± 12 nm LED during 30 min, 2.60 mW/cm2). After the treatments, the diluted samples were grown on Sabouraud Dextrose Agar and maintained at 37 °C for 24 h. Later, one single concentration was defined and the inoculum was treated with the compounds in different media (water, phosphate buffer saline – PBS, physiological solution – 0.90 % NaCl, urea 1.00 mol/L, and sodium dodecyl sulfate 0.25 % - SDS), at 20.00 mg/L for AA and AB, and at 0.50 mg/L for DMMB. The cell uptake and the dimer to the monomer ratios were determined by spectrophotometry. Results PACT with the PDs showed a reduction in the CFU/mL, with DMMB being the most effective due to the higher cell uptake within the series. When PACT was applied in the different mediums, the inactivation response was influenced by the medium. In water, the physiological solution, PBS, and urea showed no significant differences in relation to the control group. On the other hand, SDS potentiated the PACT action, and the inactivation of the microorganism was achieved with AB and DMMB. Conclusions In water, DMMB presented the highest PACT action within the PDs being studied, reaching a microorganism inactivation with a 1.00 mg/L solution, mainly due to its high cell uptake. SDS 0.25 % increased the PACT action of the PDs studied, mainly with DMMB and AB, and this may be used in formulations to develop antimicrobial treatments to be used in clinics.
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- 2019
23. Oral hygiene associated with antimicrobial photodynamic therapy or lingual scraper in the reduction of halitosis after 90 days follow up: A randomized, controlled, single-blinded trial
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Lara Jansiski Motta, Marcia Pinto Alves Mayer, Raquel Agnelli Mesquita Ferrari, Thayná Gomes Silva, Katia Llanos do Vale, Vanessa Gomes Remolina, Sandra Kalil Bussadori, Kristianne Porta Santos Fernandes, Sergio Dos Santos Romero, Renata Matalon Negreiros, Anna Carolina Ratto Tempestini Horliana, Karen Müller Ramalho, Tânia Oppido Schalch, and Ellen Sayuri Ando
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Adult ,Future studies ,Red laser ,medicine.medical_treatment ,media_common.quotation_subject ,030303 biophysics ,Biophysics ,Dentistry ,Photodynamic therapy ,Dermatology ,Oral hygiene ,030207 dermatology & venereal diseases ,03 medical and health sciences ,Dental floss ,0302 clinical medicine ,Anti-Infective Agents ,Tongue ,Hygiene ,medicine ,Humans ,Pharmacology (medical) ,media_common ,0303 health sciences ,Photosensitizing Agents ,CROMATOGRAFIA LÍQUIDA ,business.industry ,Halitosis ,Oral Hygiene ,Antimicrobial ,medicine.anatomical_structure ,Photochemotherapy ,Oncology ,business - Abstract
Background Although antimicrobial photodynamic therapy (aPDT) can reduce halitosis immediately after application, it returns after a week. This probably occurs because bacteria residing in the oral cavity may recolonize the dorsum of the tongue. Objective Verify if modification of oral hygiene behavior associated with aPDT or lingual scraper can reduce halitosis after a 90-day follow-up. Methods Forty adults with positive halitosis were randomized in G1 (n = 20) -aPDT + oral hygiene behavior (OHB) or G2 (n = 20)- lingual scraper + OHB. G1 group were submitted to 0.005 % methylene blue in the middle and posterior third of the tongue, with pre-irradiation of 1 min. Irradiations were performed with red laser diode (λ =660 nm), 100 mW, 318 J/cm2, 3537 mW/cm2, 9 J per point at 6 points. In the G2 group, the tongue was scraped 10 times on the right side and on the left side with a tongue scraper. All patients were instructed on OHB at baseline, 7 and 90 days (guidance on the use of dental floss and the Bass technique for brushing). Halitosis was evaluated by gas chromatography (OralChroma®). Values > 112 ppb for Hydrogen sulfide (H2S) gas was considered positive halitosis. Methylmercaptanes and dimethylsulfide were also measured. The gas measures were assessed at baseline, immediately, and at 7 and 90 days. Paired t-test was used for the statistical analysis. For comparison between groups, the t-test was used. Values of p Results There was no difference between groups immediately after treatment (p = .1532) after 7 days (p = 0.9312) and 90 days (p = 0.6642). For the aPDT group, there was a decrease in hydrogen sulfide immediately after treatment (p = 0.0001), after 7 days, values remained 3-fold smaller (p = 0.0088) and 2-fold smaller after 90 days (p = 0.0270). For the scraper group, there was a decrease immediately after treatment (p = 0.0001), the values remains 2-fold smaller (p = 0.0003) after 7 days and 3 months (p = 0.0001). Conclusion The oral hygiene behavior associated with aPDT or tongue scraper was not able to reduce halitosis after 90-day follow-up. Despite halitosis remaining higher than 112 ppb in all follow-up periods, the mean values remain 2 or 3 fold smaller than baseline values. Future studies should include other oral hygiene behavior to achieve better results in the treatment of halitosis.
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- 2021
24. Photodynamic therapy for endodontic treatment of primary teeth: A randomized controlled clinical trial
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Sandra Kalil Bussadori, Camila Basilio Okamoto, Renato Araujo Prates, Kristianne Porta Santos Fernandes, Anna Carolina Ratto Tempestini Horliana, Ana Carolina Costa da Mota, and Lara Jansiski Motta
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Photosensitizing Agents ,business.industry ,medicine.medical_treatment ,Root canal ,Biophysics ,Dentistry ,Photodynamic therapy ,Dermatology ,Root Canal Therapy ,Methylene Blue ,Clinical trial ,medicine.anatomical_structure ,Photochemotherapy ,Oncology ,medicine ,Deciduous teeth ,Humans ,Pharmacology (medical) ,Clinical significance ,Dental Pulp Cavity ,Tooth, Deciduous ,business ,Anterior teeth ,Permanent teeth ,Pulp necrosis - Abstract
Objectives This study aimed to evaluate the reduction in bacterial load following conventional endodontic treatment with and without antimicrobial photodynamic therapy (a-PDT) in primary teeth. Methods Thirty primary anterior teeth with a diagnosis of pulp necrosis were selected. Patients were randomly allocated to two groups as follows: Group I, patients undergoing conventional root canal therapy (n = 15) and Group II, patients undergoing conventional root canal therapy combined with antimicrobial PDT (n = 15). For PDT, methylene blue, at a concentration of 0.005 %, was used as the photosensitizing agent, which was applied to the interior of the canal with a sterile paper cone for 3 min, followed by the administration of laser light for 40 s (wavelength: 660 nm, energy density: 4 J/cm², power: 100 mW), delivered in direct contact at the entrance to the root canal. Two microbiological samples of the intra-canal content were taken (one before and one immediately after treatment in both groups) using paper cones. Clinical follow-up involved the investigation of fistulas and mobility and was performed 1- and 3 months after treatment. Data were statistically analyzed. Results The reduction in bacterial load was 93 % in Group I and 99 % in Group II, with no statistically significant difference. Conclusions Conventional treatment combined with antimicrobial PDT with parameters used in this study proved effective but presented equal efficacious capability to conventional endodontic treatment alone. Clinical significance The use of PDT has been studied in endodontic treatment protocols in permanent teeth. However, clinical trials in deciduous teeth are necessary for establishing the effectiveness and parameters of this application. The present study analyzes the results of PDT in the endodontic treatment of deciduous teeth.
- Published
- 2020
25. Antimicrobial photodynamic therapy with Bixa orellana extract and blue LED in the reduction of halitosis—A randomized, controlled clinical trial
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Sandra Kalil Bussadori, Daniela Fátima Teixeira da Silva, Marcela Leticia Leal Gonçalves, Raquel Agnelli Mesquita-Ferrari, Ana Carolina Costa da Mota, Alessandro Melo Deana, Lisyanne Araújo de Souza Cavalcante, Lara Jansiski Motta, Kristianne Porta Santos Fernandes, Anna Carolina Ratto Tempestini Horliana, Pamella de Barros Motta, Renato Araujo Prates, and Christiane Pavani
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medicine.medical_treatment ,030303 biophysics ,Biophysics ,Dentistry ,Photodynamic therapy ,Dermatology ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Anti-Infective Agents ,Tongue ,medicine ,Humans ,Pharmacology (medical) ,0303 health sciences ,Photosensitizing Agents ,biology ,Plant Extracts ,business.industry ,Bixaceae ,Halitosis ,Antimicrobial ,biology.organism_classification ,Clinical trial ,Bixa ,medicine.anatomical_structure ,Photochemotherapy ,Oncology ,business ,Brazil ,After treatment - Abstract
Background This study aimed to evaluate the reduction of halitosis when using antimicrobial photodynamic therapy (aPDT) with Bixa orellana extract and blue light-emitting diode (LED). Methods Forty-four UNINOVE students or employees with a diagnosis of sulfide (H2S) ≥ 112 ppb in gas chromatography were selected. The patients were randomly divided in groups: Group 1 (n = 15): aPDT with annatto and LED; Group 2 (n = 14): tongue scraping; Group 3 (n = 15): tongue scraping and aPDT. For aPDT, a wasBixa orellana extract used in a concentration of 20 % w/v (Formula e Acao®, Sao Paulo, Brazil) on the tongue for 2 min, associated with a blue-violet LED (Valo Cordless Ultradent® Products, Inc., South Jordan, UT, USA) (395−480 nm). Six points were irradiated on the back of the tongue, at wavelength 395−480 nm for 20 s, energy of 9.6 J and radiant energy of 6.37 J/cm2 per point. The results were compared before, immediately after treatment and 7 days after. The Friedman test was used for the intragroup analysis and the Kruskal Wallis test for the intergroup analysis. Results In all groups, there was a difference between baseline and the value immediately after the treatment. In Groups 1 and 3, there was no difference between the baseline and the 7 days control. Conclusion There was an immediate reduction of halitosis, but the reduction was not maintained after 7days.
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- 2020
26. The role of periodontal treatment associated with photodynamic therapy on the modulation of systemic inflammation in the experimental model of asthma and periodontitis
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Sandra Kalil Bussadori, Jessica Gonzaga Oliveira, Adriana Lino-dos-Santos-Franco, Felipe Murakami-Malaquias-da-Silva, Marlon Palma-Cruz, Renata Matalon Negreiros, João Gabriel Fernandes da Silva, Ana Paula Ligeiro-de-Oliveira, Nicole Cristine Rigonato-Oliveira, Isabella Sena Avelar, Anna Carolina Ratto Tempestini Horliana, and Ellen Perim Rosa
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Male ,medicine.medical_specialty ,medicine.medical_treatment ,030303 biophysics ,Biophysics ,Photodynamic therapy ,Dermatology ,Systemic inflammation ,Bronchoalveolar Lavage ,Gastroenterology ,Root Planing ,Flow cytometry ,Mice ,030207 dermatology & venereal diseases ,03 medical and health sciences ,Basal (phylogenetics) ,0302 clinical medicine ,Internal medicine ,Animals ,Medicine ,Pharmacology (medical) ,Platelet ,Periodontitis ,Asthma ,Mice, Inbred BALB C ,0303 health sciences ,Photosensitizing Agents ,medicine.diagnostic_test ,Platelet Count ,Tumor Necrosis Factor-alpha ,business.industry ,medicine.disease ,humanities ,Methylene Blue ,Photochemotherapy ,Oncology ,Dental Scaling ,Differential Leukocyte Count ,Lasers, Semiconductor ,medicine.symptom ,business - Abstract
The association of Periodontitis (P) with several systemic diseases, among them asthma (A), has been previously studied. As periodontal treatment (TTO) associated with photodynamic therapy (PDT) is able to treat P, the aim of this study is to verify whether periodontitis exerts systemic effects on asthma, and whether TTO, associated or not with PDT, is capable of altering the systemic course of both pathologies.64 male Balb/c mice were divided into 8 groups (n = 8): Basal (B), P, P + TTO, P + TTO + PDT, Asthma, A + P, A + P + TTO, A + P + TTO + PDT. After 43 days, all animals were euthanized. The total and differential leukocyte count in serum, platelet count, alveolar bronchial lavage cell count, femoral lavage cell count in addition to the reactivity of the trachea, lung edema and gingiva cytokines were analyzed. The frequency of inflammatory cells was assessed via flow cytometry. One-way ANOVA test was used, followed by the Student-Newman-Keuls post-test.There was an increase in the number of blood circulating eosinophils in group A when compared to group B (p 0.01); this characterized the asthma experimental model. P (p 0.05) presented a lower amount of cytokine TNF-α in the gingiva when compared to the Asthma group. Apart from that, there was no statistical difference found for the other analyzed parameters.These data contributed to elucidate that P and A, associated or not with TTO and PDT, are not able to interfere with the systemic parameters of Balb/c mice.
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- 2020
27. 'Efficacy of photodynamic therapy on the treatment of herpes labialis: A systematic review'
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Lara Jansiski Motta, Thalita Santana, Aline Campos de Queiroz, Mônica Andrade Lotufo, Raquel Agnelli Mesquita Ferrari, Andréa Oliver Gomes, Sandra Kalil Bussadori, Kristianne Porta Santos Fernandes, and Anna Carolina Ratto Tempestini Horliana
- Subjects
medicine.medical_specialty ,medicine.medical_treatment ,Biophysics ,Photodynamic therapy ,Dermatology ,law.invention ,Randomized controlled trial ,Recurrence ,law ,medicine ,Humans ,Pharmacology (medical) ,Low-Level Light Therapy ,Adverse effect ,Herpes Labialis ,Protocol (science) ,Photosensitizing Agents ,business.industry ,Checklist ,Clinical trial ,Systematic review ,Photochemotherapy ,Oncology ,Patient Satisfaction ,business - Abstract
Objective We conducted a systematic review to evaluate the efficacy of PDT in the management of recurrent herpes labialis (RHL). Study design: systematic review. Methods This study was reported according to the PRISMA checklist and we performed a literature search on five databases. Results The search revealed that there are no published clinical trials addressing PDT on RHL, therefore we conducted a review of case reports and five studies were included for qualitative review. The number of treated patients varied from 2 to 6 in each article. Most studies used methylene blue as a photosensitizer, while one used 5-aminolevulinic acid. For light irradiation, most studies used laser and one used a red light from halogen lamp. Patients’ follow-up varied from 24 h to 12 months. All articles reported good outcomes with resolution of disease and no recurrences. Only one study reported adverse effects during treatment (burning and pain). Conclusion The results of this review suggest that PDT could be an effective treatment for herpes labialis. However, due to very few case reports and heterogeneity among protocols, there is a call for well-designed randomized clinical trials to confirm the efficacy of this therapy and to establish standardized protocols. The review protocol was registered at the International Prospective Register of Systematic Reviews (PROSPERO) under number CRD CRD42018108973.
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- 2020
28. Parameters for antimicrobial photodynamic therapy on periodontal pocket—Randomized clinical trial
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Alvarenga, Letícia H., primary, Gomes, Ana Carolina, additional, Carribeiro, Pamela, additional, Godoy-Miranda, Bianca, additional, Noschese, Giovane, additional, Simões Ribeiro, Martha, additional, Kato, Ilka Tiemy, additional, Bussadori, Sandra Kalil, additional, Pavani, Christiane, additional, Geraldo, Yhago Gazote Eloy, additional, Silva, Daniela de Fátima Teixeira da, additional, Horliana, Anna Carolina Ratto Tempestini, additional, Wainwright, Mark, additional, and Prates, Renato Araujo, additional
- Published
- 2019
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