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Aim: To test a chemical-mechanical method and antimicrobial photodynamic therapy (a-PDT) in combination to manage a deep dental carious lesion in a permanent molar pediatric patient., Background: Due to specific characteristics of early-erupted teeth, as first permanent lower molars, techniques that provide less invasive tissue removal aiming keep healthy tooth parts, such as pulp tissue, are desired. Furthermore, especially to children, provide comfort, less noise and vibration are important issues that should be considered on the management of deep caries., Case Description: The following case report presents an association of Papacarie Duo® (PD) and a-PDT used in a 9-year-old patient with a deep dental caries in the mandibular right first molar. After PD application, the carious tissue was carefully removed with the cavity stained for 1 min with rose bengal solution, irradiated with a high intensity LED constituting the a-PDT technique, and definitively restored with composite resin. At 6 months of follow-up, no signs of caries were verified, showing success to applied techniques. A microbiological evaluation attested the antimicrobial effects of the alternative procedures., Conclusion: Although it reached optimal clinical and bacterial reduction outcomes, the approach demonstrated an excessive length of time taken for the management of carious lesion., Clinical Significance: The use of new technologies should be encouraged, especially when treating deep caries aiming for a less invasive approach., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Antimicrobial photodynamic therapy (aPDT) has emerged as a promising antimicrobial treatment to control microorganisms including those involved in oral diseases, especially dental caries. Hence, the aim of this study was to evaluate the influence of aPDT - pre-irradiation time (PIT), at different periods, on antimicrobial rate of Streptococcus mutans (S. mutans). A standard suspension of S. mutans UA159 was prepared and submitted at sensitization of 0.005 % methylene blue (MB) for 0, 1, 3 and 5 min (G1 - G4 groups, respectively) and irradiated with a red laser (660 nm; 321 J/cm 2 ; 9 J; 90 s) afterward. A control group using PBS instead of MB was performed as well (G5). The number of colony-forming units (CFU)/mL was recorded, transformed into log 10 and analyzed by ANOVA and Tukey's test at a cutoff value at 0.05. Overall, the aPDT groups tested achieved a bacterial reduction > 1-log 10 when compared to G5 (p < 0.05) with no statistical difference among the different PIT tested. The need of PIT before aPDT application deserves attention, since its time reduction implies on shorter clinical approaches without compromising the photodynamic antibacterial efficacy in the in vitro parameters employed., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Background: The failure of endodontic treatment is linked to the presence of microorganisms, particularly Enterococcus faecalis, in the root canals. This study evaluated the effectiveness of photodynamic therapy (PDT) using erythrosine irradiated by a high-power curing light on a planktonic suspension culture of E. faecalis., Methods: Bacterial suspensions of E. faecalis were adjusted and then mixed in a 1:1 proportion, in triplicate, in treatment groups by varying the length of irradiation time (120 and 240s) and the molarity of the erythrosine (5 and 10μM). In order to verify the post-treatment bactericidal effect, a count of the viable bacteria was performed (CFUmL -1 ) and transformed into Log10 CFU. The one-way ANOVA with Tukey post-hoc test was applied to check for differences between the groups., Results: The bacteria were completely eradicated in the groups that used PDT with 5μM 240s, 10μM 120s and 10μM 240s (p≪0.001). The effect of the PDT 5μM 120s group was significant (p≪0.05) in comparison with the groups using only light or only erythrosine. Positive control (exposure to 2.5% NaClO for 120 and 240s) completely eradicated E. faecalis. The negative control (PBS) did not alter the quantities of E. faecalis CFU with 9.605 Log10 CFU at 120s and 9.621 Log10 CFU at 240s., Conclusion: PDT with erythrosine in a concentration of 10μM and high-power LED is capable of totally eliminating E. faecalis in planktonic suspension., (Copyright © 2017 Elsevier B.V. All rights reserved.)