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We prepared spiro rhodamine (RB)-coumarin (Cou) compact electron donor–acceptor dyads (RB-Cou-CF3 and RB-Cou-CN), to study the charge transfer (CT) and spin–orbit CT intersystem crossing (SOCT-ISC). The π-conjugation planes of the rhodamine and coumarin units in both dyads are in nearly orthogonal geometry (dihedral angle: 86.3°). CT state emission was observed for RB-Cou-CF3 (at 550 nm) and RB-Cou-CN (at 595 nm). Although the fluorescence of the pristine coumarin units (fluorescence quantum yields ΦF = 59%) was quenched in the dyads (ΦF = 0.5 ~ 1.1% in n-hexane), the triplet state quantum yields of the dyads are also low (singlet oxygen quantum yield, ΦΔ = 2.3–7.5% in n-hexane). Nanosecond transient absorption spectra show that the 3Cou* state was formed, which shows a triplet state lifetime of 11–15.6 μs. The proposed photophysical path for the dyads is as follows: RB-1Cou* → RB+•-Cou−• → RB-3Cou*. The low SOCT-ISC yield is attributed to the slightly lower charge-transfer state energy (1.94 eV in toluene) as compared to the 3Cou* state energy (2.23 eV) and the shallow potential energy curve (PEC) at energy minima of the dyads. This work indicates that orthogonal conformation of donor–acceptor units is inadequate for achieving efficient SOCT-ISC. These results are useful for studying charge separation and intersystem crossing of electron donor/acceptor dyads. [ABSTRACT FROM AUTHOR]

The rise of antibacterial drug resistance means treatment options are becoming increasingly limited. We must find ways to tackle these hard-to-treat drug-resistant and biofilm infections. With the lack of new antibacterial drugs (such as antibiotics) reaching the clinics, research has switched focus to exploring alternative strategies. One such strategy is antibacterial photodynamic therapy (aPDT), a system that relies on light, oxygen, and a non-toxic dye (photosensitiser) to generate cytotoxic reactive oxygen species. This technique has already been shown capable of handling both drug-resistant and biofilm infections but has limited clinical approval to date, which is in part due to the low bioavailability and selectivity of hydrophobic photosensitisers. Nanotechnology-based techniques have the potential to address the limitations of current aPDT, as already well-documented in anti-cancer PDT. Here, we review recent advances in nanoparticle-based targeting tactics for aPDT. [ABSTRACT FROM AUTHOR]

A novel tricationic Zn(II)phthalocyanine derivative, (NCH3)3ZnPc3+, was synthesized by ring expansion reaction of boron(III) [2,9(10),16(17)-trinitrosubphthalocyaninato]chloride. First, the reaction of this subphthalocyanine with 2,3-naphthalenedicarbonitrile and Zn(CH3COO)2 catalyzed by 8-diazabicyclo[5.4.0]undec-7-ene was used to obtain the A3B-type nitrophthalocyanine. After reduction of nitro groups with Na2S and exhaustive methylation of amino groups, (NCH3)3ZnPc3+ was formed in good yields. In addition, the tetracationic analog (NCH3)4ZnPc4+ was synthesized to compare their properties. The absorption and fluorescence spectra showed the Q-bands and the red emission, respectively, which are characteristic of the Zn(II)phthalocyanine derivatives in N,N-dimethylformamide. Furthermore, photodynamic activity sensitized by these compounds was studied in the presence of different molecular probes to sense the formation of reactive oxygen species. (NCH3)3ZnPc3+ efficiently produced singlet molecular oxygen and also it sensitized the formation of superoxide anion radical in the presence of NADH, while the photodynamic activity of (NCH3)4ZnPc4+ was very poor, possibly due to the partial formation of aggregates. Furthermore, the decomposition of L-tryptophan induced by (NCH3)3ZnPc3+ was mainly mediated by a type II mechanism. Antimicrobial photodynamic inactivation sensitized by these phthalocyanines was evaluated in Staphylococcus aureus, Escherichia coli, and Candida albicans, as representative microbial cells. In cell suspensions, (NCH3)3ZnPc3+ was rapidly bound to microbial cells, showing bioimages with red fluorescence emission. After 5 min of irradiation with visible light, (NCH3)3ZnPc3+ was able to completely eliminate S. aureus, E. coli and C. albicans, using 1.0, 2.5 and 5.0 μM phthalocyanine, respectively. In contrast, a low photoinactivation activity was found with (NCH3)4ZnPc4+ as a photosensitizer. Therefore, the amphiphilic tricationic phthalocyanine (NCH3)3ZnPc3+ is a promising photosensitizing structure for application as a broad-spectrum antimicrobial phototherapeutic agent. [ABSTRACT FROM AUTHOR]

The Environmental Effects Assessment Panel (EEAP) is one of three Panels that regularly informs the Parties (countries) to the Montreal Protocol on the effects of ozone depletion and the consequences of climate change interactions with respect to human health, animals, plants, biogeochemistry, air quality, and materials. The Panels provide a detailed assessment report every four years. The most recent 2014 Quadrennial Assessment by the EEAP was published as a special issue of seven papers in 2015 (Photochem. Photobiol. Sci., 2015, 14, 1–184). The next Quadrennial Assessment will be published in 2018/2019. In the interim, the EEAP generally produces an annual update or progress report of the relevant scientific findings. The present progress report for 2015 assesses some of the highlights and new insights with regard to the interactive nature of the effects of UV radiation, atmospheric processes, and climate change. [ABSTRACT FROM AUTHOR]