1. Effect of Reserpine Pretreatment on Avian Erythrocyte Carbonic Anhydrase Activation by Isoproterenol
- Author
-
Alexander D. Kenny, Ingrid M. Greene, Immaculata N.A. Igbo, and Charles E. Reigel
- Subjects
Male ,medicine.medical_specialty ,Erythrocytes ,Reserpine ,Coturnix ,Propranolol ,Carbonic anhydrase ,Internal medicine ,Isoprenaline ,medicine ,Animals ,Carbonic Anhydrases ,Pharmacology ,chemistry.chemical_classification ,biology ,Chemistry ,Body Weight ,Isoproterenol ,General Medicine ,Enzyme Activation ,Endocrinology ,Epinephrine ,Enzyme ,biology.protein ,Catecholamine ,Hemoglobin ,medicine.drug - Abstract
We studied the action of beta-adrenergic agonists on Japanese quail erythrocyte carbonic anhydrase (CA) in vitro. Earlier we had reported that epinephrine increased CA activity by 14%; the present study focused on an attempt to increase the size of this response. Washed erythrocytes from reserpine-treated (1 mg/kg daily i.m. for 3 days) and control birds were incubated for 40 min in the presence of isoproterenol 10(-6) mol/l. The activity of CA expressed as Wilbur-Anderson units/mg hemoglobin was increased by as much as 42% in reserpine-treated birds over the control depending on the conditions. Addition of 10(-5) mol/l of the beta-adrenergic antagonist propranolol inhibited the isoproterenol-induced effect in nonreserpinized birds. We conclude that pretreatment with reserpine, which was accompanied by a fall in plasma catecholamine levels, particularly epinephrine levels, enhanced the activation of CA by isoproterenol.
- Published
- 1994