1. Autologous Marrow Mesenchymal Stem Cell Driving Bone Regeneration in a Rabbit Model of Femoral Head Osteonecrosis.
- Author
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Mastrolia, Ilenia, Giorgini, Andrea, Murgia, Alba, Loschi, Pietro, Petrachi, Tiziana, Rasini, Valeria, Pinelli, Massimo, Pinto, Valentina, Lolli, Francesca, Chiavelli, Chiara, Grisendi, Giulia, Baschieri, Maria Cristina, Santis, Giorgio De, Catani, Fabio, Dominici, Massimo, and Veronesi, Elena
- Subjects
BONE regeneration ,MESENCHYMAL stem cells ,FEMUR head ,TOTAL hip replacement ,OSTEONECROSIS ,BONE growth ,BONE cells - Abstract
Osteonecrosis of the femoral head (ONFH) is a progressive degenerative disease that ultimately requires a total hip replacement. Mesenchymal stromal/stem cells (MSCs), particularly the ones isolated from bone marrow (BM), could be promising tools to restore bone tissue in ONFH. Here, we established a rabbit model to mimic the pathogenic features of human ONFH and to challenge an autologous MSC-based treatment. ON has been originally induced by the synergic combination of surgery and steroid administration. Autologous BM-MSCs were then implanted in the FH, aiming to restore the damaged tissue. Histological analyses confirmed bone formation in the BM-MSC treated rabbit femurs but not in the controls. In addition, the model also allowed investigations on BM-MSCs isolated before (ON-BM-MSCs) and after (ON+BM-MSCs) ON induction to dissect the impact of ON damage on MSC behavior in an affected microenvironment, accounting for those clinical approaches foreseeing MSCs generally isolated from affected patients. BM-MSCs, isolated before and after ON induction, revealed similar growth rates, immunophenotypic profiles, and differentiation abilities regardless of the ON. Our data support the use of ON+BM-MSCs as a promising autologous therapeutic tool to treat ON, paving the way for a more consolidated use into the clinical settings. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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