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4 results on '"Prow, Tarl"'

3. Gold Nanoparticle Penetration and Reduced Metabolism in Human Skin by Toluene

Author

Margaret K. Butler, David Liu, Hagar I. Labouta, H. Peter Soyer, Marc Schneider, Tobias Kraus, Lynlee L. Lin, Tarl W. Prow, Michael S. Roberts, Anthony P. Raphael, Labiba K. El-Khordagui, Jeffrey E. Grice, Labouta, Hagar I, Liu, David C, Lin, Lynlee L, Butler, Margaret K, Grice, Jeffrey E, Raphael, Anthony P, Kraus, Tobias, El-Khordagui, Labiba K, Soyer, H Peter, Roberts, Michael S, Schneider, Marc, and Prow, Tarl W

Subjects
skin, Drug Compounding, Skin Absorption, Drug Evaluation, Preclinical, Metal Nanoparticles, Pharmaceutical Science, Nanoparticle, Human skin, Administration, Cutaneous, Photochemistry, Permeability, chemistry.chemical_compound, Drug Delivery Systems, fluorescence lifetime, Humans, Pharmacology (medical), Particle Size, Skin, Pharmacology, confocal reflectance microscopy, Aqueous solution, integumentary system, nanoparticle, Organic Chemistry, Metabolism, Penetration (firestop), Toluene, chemistry, Colloidal gold, multiphoton microscopy, Solvents, Molecular Medicine, Gold, Particle size, Epidermis, NADP, Biotechnology
Abstract

Purpose: To measure penetration and metabolic effects of ionstabilized, polar, 15 nm gold nanoparticles in aqueous solution (AuNP-Aq) and sterically stabilized, non-polar, 6 nm gold nanoparticles in toluene (AuNP-TOL) on excised human skin. Methods: Gold nanoparticles were characterized with dynamic light scattering and transmission electron microscopy (TEM). Skin penetration studies were done on frozen or fresh excised skin using static Franz diffusion cells. Viable treated skin was assessed by dermoscopy, reflectance confocal microscopy (RCM), multiphoton tomography (MPT) with fluorescence lifetime imaging microscopy (FLIM), and TEM. Results: Dermoscopy and RCM showed large aggregates in the furrows of AuNP-Aq-treated skin. Treatment of thawed and viable skin only showed enhanced permeability to nanoparticles in the AuNP-TOL group with MPT and FLIM imaging to stratum spinosum of epidermis. TEM analysis revealed gold nanoparticles within AuNP-treated stratum corneum. FLIM analysis of NAD(P)H showed a significant decrease in total NAD(P)H in all toluenetreated groups. Conclusions: Gold nanoparticles, 15 nm, in aqueous solution aggregated on the skin surface. Toluene treatment eliminated skin metabolism; skin treated with toluene/gold nanoparticles (6 nm) for 24 h, but not at 4 h, showed increased nanoparticle permeability. These results are of value to nanotoxicology. Refereed/Peer-reviewed

Published
2011

4. Time-Correlated Single Photon Counting For Simultaneous Monitoring Of Zinc Oxide Nanoparticles And NAD(P)H In Intact And Barrier-Disrupted Volunteer Skin

Author

Thomas Robertson, Tarl W. Prow, Andrei V. Zvyagin, Lynlee L. Lin, H. Peter Soyer, Michael S. Roberts, Jeffrey E. Grice, Wolfgang Becker, Margaret K. Butler, Lin, Lynlee L, Grice, Jeffrey E, Butler, Margaret K, Zvyagin, Andrei V, Becker, Wolfgang, Robertson, Thomas A, Soyer, H Peter, Roberts, Michael S, and Prow, Tarl W

Subjects
Time Factors, Administration, Topical, Skin Absorption, Analytical chemistry, Metal Nanoparticles, Pharmaceutical Science, Nanoparticle, chemistry.chemical_element, Human skin, Cosmetics, Zinc, Dermatitis, Atopic, Drug Delivery Systems, Toxicity Tests, Humans, Psoriasis, Pharmacology (medical), Surgical Tape, Volunteer, Skin, Pharmacology, Photons, sunscreen, Dose-Response Relationship, Drug, Chemistry, Organic Chemistry, Water Loss, Insensible, Photon counting, zinc oxide nanoparticle, Dose–response relationship, Toxicity, Biophysics, Molecular Medicine, NAD+ kinase, Zinc Oxide, human skin, multiphotonmicroscopy, Sunscreening Agents, metabolism, NADP, Biotechnology
Abstract

Purpose There is a lack of relevant, non-animal alternatives for assessing exposure and toxicity of nanoparticle-containing cosmetics, e.g. sunscreens. Our goal was to evaluate timecorrelated single photon counting (TCSPC) for simultaneous monitoring of zinc oxide nanoparticles (ZnO-NP) and the metabolic state of volunteer skin. Methods We separated the fluorescence lifetime signatures of endogenous fluorophore signals (i.e. nicotinamide adenine dinucleotide phosphate, NAD(P)H and keratin) and the ZnO-NP signal using advanced TCSPC to simultaneously determine ZnO-NP penetration profiles and NAD(P)H changes in subjects with altered barrier function, including tape-stripped skin and in psoriasis or atopic dermatitis lesions. Results We detected no ZnO-NP penetration into viable human skin in any group. ZnO-NP signal was significantly increased (p

Published
2011

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