1. Neonicotinoid insecticides mode of action on insect nicotinic acetylcholine receptors using binding studies
- Author
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Andrew K. Jones, Emiliane Taillebois, Steeve H. Thany, Alison Cartereau, Université Francois Rabelais [Tours], Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Institut National de la Recherche Agronomique (INRA)-Université d'Orléans (UO), and Oxford Brookes University
- Subjects
0301 basic medicine ,nicotinic receptors ,Insecticides ,[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,binding studies ,Health, Toxicology and Mutagenesis ,media_common.quotation_subject ,Insect ,Receptors, Nicotinic ,Neonicotinoids ,03 medical and health sciences ,0302 clinical medicine ,neonicotinoid insecticides ,Animals ,Humans ,Binding site ,Mode of action ,media_common ,Acetylcholine receptor ,Binding Sites ,Chemistry ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Binding properties ,Neonicotinoid ,General Medicine ,3. Good health ,030104 developmental biology ,Nicotinic agonist ,nervous system ,Biochemistry ,insect ,Whole body ,Agronomy and Crop Science ,030217 neurology & neurosurgery ,Protein Binding - Abstract
International audience; Nicotinic acetylcholine receptors (nAChRs) are the main target of neonicotinoid insecticides, which are widely used in crop protection against insect pests. Electrophysiological and molecular approaches have demonstrated the presence of several nAChR subtypes with different affinities for neonicotinoid insecticides. However, the precise mode of action of neonicotinoids on insect nAChRs remains to be elucidated. Radioligand binding studies with [3H]-α-bungarotoxin and [3H]-imidacloprid have proved instructive in understanding ligand binding interactions between insect nAChRs and neonicotinoid insecticides. The precise binding site interactions have been established using membranes from whole body and specific tissues. In this review, we discuss findings concerning the number of nAChR binding sites against neonicotinoid insecticides from radioligand binding studies on native tissues. We summarize the data available in the literature and compare the binding properties of the most commonly used neonicotinoid insecticides in several insect species. Finally, we demonstrate that neonicotinoid-nAChR binding sites are also linked to biological samples used and insect species.
- Published
- 2018