1. Cross-resistance pattern to ACCase-inhibiting herbicides in a novel Trp1999Leu mutation American sloughgrass (Beckmannia syzigachne) population.
- Author
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Liu, Bingqi, Ding, Fei, Wang, Mingliang, Wang, Fei, Luo, Xiaoyong, and Li, Lingxu
- Subjects
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HERBICIDES , *HERBICIDE resistance , *POPULATION , *INSECTICIDE resistance - Abstract
The plastid acetyl coenzyme carboxylase (ACCase) Trp 1999 Leu mutation was identified in a Beckmannia syzigachne population resistant to fenoxaprop-p-ethyl. The pattern of cross-resistance for the Trp 1999 Leu mutation is still ambiguous. In this paper, mutant homozygote (1999Leu/Leu, RR) and wild type (1999Trp/Trp, SS) B. syzigachne plants with the same genetic background were purified from the JS-26 population using the dCAPS method. The activity of ACCase in RR and SS was determined. Then, the cross-resistance pattern to ACCase inhibiting herbicides of the Trp 1999 Leu mutation was determined using the whole-plant method. ACCase activity showed that the Trp 1999 Leu mutation decreased ACCase sensitivity to fenoxaprop-p-ethyl by 2.73-fold. A dose-response experiment indicated that the Trp 1999 Leu mutation conferred high resistance to quizalofop-p-ethyl (20.29-fold), metamifop (12.22-fold) and pinoxaden (18.60-fold), moderate resistance to fenoxaprop-p-ethyl (8.20-fold) and sethoxydim (6.38-fold), low resistance to cyhalofop-butyl (2.73-fold) and no resistance to clodinafop-propargyl (1.42 fold) and clethodim (1.59-fold). This is the first report of the role of Trp 1999 Leu in fenoxaprop-p-ethyl resistance and of the patterns of cross-resistance to ACCase-inhibiting herbicides in B. syzigachne. Unlabelled Image • A novel W 1999 L mutation in ACCase was identified in fenoxaprop resistant Beckmannia syzigachne. • W 1999 L mutation resulted in 2.73-folds decrease on ACCase sensitivity to fenoxaprop-p-ethyl. • W 1999 L mutation mutation conferred moderate to high or no to low resistance to APPs with two-rings or one-ring aromatic moiety. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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