4 results on '"somatic tissue"'
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2. In vitro culture of somatic cells derived from ear tissue of collared peccary (Pecari tajacu Linnaeus, 1758) in medium with different requirements.
- Author
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Santos, Magda L. T., Borges, Alana A., Queiroz Neta, Luiza B., Santos, Maria V. O., Oliveira, Moacir F., Silva, Alexandre R., and Pereira, Alexsandra F.
- Abstract
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- Published
- 2016
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3. In vitro culture of somatic cells derived from ear tissue of collared peccary (Pecari tajacu Linnaeus, 1758) in medium with different requirements
- Author
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Magda L.T. Santos, Alana A. Borges, Luiza B. Queiroz Neta, Maria V.O. Santos, Moacir F. Oliveira, Alexandre R. Silva, and Alexsandra F. Pereira
- Subjects
Somatic cells ,collared peccary ,Pecari tajacu ,culture medium ,wild animals ,conservation ,somatic tissue ,protein source ,mitotic factor. ,Veterinary medicine ,SF600-1100 - Abstract
ABSTRACT: The maintenance of metabolic activities during the in vitro culture of somatic cells of wild animals, especially collared peccary (Pecari tajacu), is an interesting step in conservation of these cells for the use in nuclear transfer. In this context, it is necessary to optimize the culture conditions of somatic cells by the establishment of appropriate supplementation to the media. Therefore, this study aimed to analyze the composition of the culture means of somatic cell derived from ear tissue of collared peccaries, evaluating concentrations of fetal bovine serum (FBS; 10% vs. 20%) and epidermal growth factor (EGF; 5ng/mL vs. 10ng/mL). Tissues were submitted to primary culture and subcultures for 40 days and cells were analyzed for morphology, adhesion, subconfluence, and proliferative activity to develop the growth curve and to determine the population doubling time (PDT), viability, and functional/metabolic activity. No difference was observed between the concentrations of FBS for several parameters, except for viability [FBS10: 85.6% vs. FBS20: 98.2%], PDT [FBS10: 155.4h vs. 77.2h], and functional/metabolic assay [FBS10: 0.57-0.55 vs. FBS20: 0.82-0.99 (D5-D7)]. For the EGF in culture, no difference was observed in the evaluated parameters. In all experiments, the growth curves were typical S-shape and the cells passed through a lag, logarithmic, and plateau phase. In conclusion, 20% FBS is suitable for the recovery of somatic cells; nevertheless, EGF does not improve the quality of growing these cells. To our knowledge, this is the first study culturing somatic cells of collared peccaries.
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4. Cultivo in vitro de células somáticas derivadas de tecido auricular de cateto (Pecari tajacu Linnaeus, 1758) em meio com diferentes requerimentos
- Author
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Alana Azevedo Borges, Luiza Bento de Queiroz Neta, Moacir Franco de Oliveira, Alexandre Rodrigues Silva, M. L. T. Santos, Alexsandra Fernandes Pereira, and María Victoria Santos
- Subjects
0301 basic medicine ,Pecari ,Pecari tajacu ,culture medium ,somatic tissue ,Somatic cell ,fator mitótico ,Context (language use) ,Biology ,mitotic factor ,Andrology ,03 medical and health sciences ,tecido somático ,Epidermal growth factor ,wild animals ,tecido auricular ,Doubling time ,conservação ,Somatic cells ,protein source ,fonte proteica ,lcsh:Veterinary medicine ,General Veterinary ,meio de cultivo ,0402 animal and dairy science ,conservation ,collared peccary ,04 agricultural and veterinary sciences ,Growth curve (biology) ,biology.organism_classification ,040201 dairy & animal science ,In vitro ,animais silvestres ,030104 developmental biology ,catetos ,Células somáticas ,Immunology ,lcsh:SF600-1100 ,Fetal bovine serum - Abstract
The maintenance of metabolic activities during the in vitro culture of somatic cells of wild animals, especially collared peccary (Pecari tajacu), is an interesting step in conservation of these cells for the use in nuclear transfer. In this context, it is necessary to optimize the culture conditions of somatic cells by the establishment of appropriate supplementation to the media. Therefore, this study aimed to analyze the composition of the culture means of somatic cell derived from ear tissue of collared peccaries, evaluating concentrations of fetal bovine serum (FBS; 10% vs. 20%) and epidermal growth factor (EGF; 5ng/mL vs. 10ng/mL). Tissues were submitted to primary culture and subcultures for 40 days and cells were analyzed for morphology, adhesion, subconfluence, and proliferative activity to develop the growth curve and to determine the population doubling time (PDT), viability, and functional/metabolic activity. No difference was observed between the concentrations of FBS for several parameters, except for viability [FBS10: 85.6% vs. FBS20: 98.2%], PDT [FBS10: 155.4h vs. 77.2h], and functional/metabolic assay [FBS10: 0.57-0.55 vs. FBS20: 0.82-0.99 (D5-D7)]. For the EGF in culture, no difference was observed in the evaluated parameters. In all experiments, the growth curves were typical S-shape and the cells passed through a lag, logarithmic, and plateau phase. In conclusion, 20% FBS is suitable for the recovery of somatic cells; nevertheless, EGF does not improve the quality of growing these cells. To our knowledge, this is the first study culturing somatic cells of collared peccaries. RESUMO: A manutenção das atividades metabólicas durante o cultivo in vitro de células somáticas de animais silvestres, especialmente cateto (Pecari tajacu), é uma etapa interessante na conservação dessas células para o uso na transferência nuclear. Nesse contexto, é necessário aperfeiçoar as condições de cultivo de células somáticas pelo estabelecimento de suplementações apropriadas aos meios. Portanto, este estudo objetivou analisar a composição dos meios de cultivo de células somáticas derivadas de tecido auricular de catetos, avaliando concentrações de soro fetal bovino (SFB; 10% vs. 20%) e fator de crescimento epidermal (EGF; 5 ng/mL vs. 10 ng/mL). Para tanto, tecidos foram submetidos ao cultivo primário e subcultivos por 40 dias e células foram analisadas por morfologia, adesão, subconfluência, e atividade proliferativa pelo desenvolvimento da curva de crescimento e determinação do time de duplicação da população (PDT), viabilidade, e atividade funcional/metabólica. Nenhuma diferença foi observada entre as concentrações de SFB para os vários parâmetros, exceto para viabilidade [SFB10: 85,6% vs. SFB20: 98,2%], PDT [SFB10: 155,4 h vs. 77,2 h], e atividade funcional/metabólica [SFB10: 0,57-0,55 vs. SFB20: 0,82-0,99 (D5-D7)]. Para o EGF em cultivo, nenhuma diferença foi observada nos parâmetros avaliados. Em todos os experimentos, as curvas de crescimento foram típicas de forma S e as células passaram por uma fase lag, logarítmica e platô. Em conclusão, 20% de SFB é adequado para a recuperação de células somáticas; contudo, EGF não melhora a qualidade de crescimento dessas células. Ao nosso conhecimento, este é o primeiro estudo cultivando células somáticas de catetos.
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