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Haemonchus contortus is the major gastrointestinal parasite of sheep raised in tropical and subtropical areas worldwide. This trial aimed to evaluate the influence of H. contortus infection on the bone marrow response of Santa Ines (SI) and Ile de France (IF) suckling lambs experimentally infected with H. contortus. Fourteen SI lambs and 12 IF lambs were randomized in four groups: infected SI (n=8), non-infected SI (n=6), infected IF (n=8) and non-infected IF (n=4). Lambs of infected groups were submitted to 27 infections, conducted every two days, from 14 to 68 days of age, and each lamb received a total of 5400 H. contortus infective larvae (L3). Ten blood samples were obtained during the experimental period to be used for erythrocyte and leukocyte counts, packed cell volume and total plasma protein estimation. Additionally, it was carried out a differential leukocyte count. Lambs from control groups did not shed eggs in faeces all over the experiment, while infected Santa Ines and Ile de France lambs presented means of 2963 EPG (Eggs Per Gram of faeces) and 8175 EPG in the last sampling (P

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:50:39Z No. of bitstreams: 1 S0100-736X2007000100008.pdf: 693372 bytes, checksum: 6bf33ce53281f9a666eed08254885a7f (MD5) Made available in DSpace on 2013-08-22T18:50:39Z (GMT). No. of bitstreams: 1 S0100-736X2007000100008.pdf: 693372 bytes, checksum: 6bf33ce53281f9a666eed08254885a7f (MD5) Previous issue date: 2007-01-01 Made available in DSpace on 2013-09-30T19:41:46Z (GMT). No. of bitstreams: 2 S0100-736X2007000100008.pdf: 693372 bytes, checksum: 6bf33ce53281f9a666eed08254885a7f (MD5) S0100-736X2007000100008.pdf.txt: 29305 bytes, checksum: 9730a51b0d86599faa677ea2f974969b (MD5) Previous issue date: 2007-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:52:56Z No. of bitstreams: 2 S0100-736X2007000100008.pdf: 693372 bytes, checksum: 6bf33ce53281f9a666eed08254885a7f (MD5) S0100-736X2007000100008.pdf.txt: 29305 bytes, checksum: 9730a51b0d86599faa677ea2f974969b (MD5) Made available in DSpace on 2014-05-20T13:52:56Z (GMT). No. of bitstreams: 2 S0100-736X2007000100008.pdf: 693372 bytes, checksum: 6bf33ce53281f9a666eed08254885a7f (MD5) S0100-736X2007000100008.pdf.txt: 29305 bytes, checksum: 9730a51b0d86599faa677ea2f974969b (MD5) Previous issue date: 2007-01-01 Avaliaram-se a associação entre o número de células inflamatórias no intestino delgado e a resistência à infecção por Trichostrongylus colubriformis em ovinos de três raças (Santa Inês, Suffolk e Ile de France), naturalmente infectados. Mastócitos, eosinófilos e leucócitos globulares foram quantificados na mucosa intestinal. A concentração de histamina foi estimada em amostras teciduais do intestino, bem como foi determinado o comprimento de machos e fêmeas de T. colubriformis. A resposta celular foi similar na mucosa intestinal das três raças ovinas (P>0,05). Houve grande variação entre os ovinos em relação aos resultados parasitológicos e celulares, mesmo nos animais de mesma raça. em geral, os animais que apresentaram número menor de células inflamatórias tiveram cargas parasitárias maiores, contagens de ovos por grama de fezes mais altas e exemplares de T. colubriformis maiores. Os resultados indicaram que mastócitos, eosinófilos e leucócitos globulares prejudicaram o estabelecimento, o desenvolvimento e a sobrevivência dos parasitas. The study was carried out to evaluate the relationship of inflammatory intestinal cells with the resistance to Trichostrongylus colubriformis infections in three breeds of sheep (Santa Ines, Suffolk and Ile de France), naturally infected. Mast cells, eosinophils, and globule leucocytes were enumerated in intestinal mucosa. Histamine concentration was estimated in intestinal tissue samples and the length of male and female specimens were determined. The three breeds of sheep showed similar cellular response in the small intestine mucosa (P>0.05). There was extensive variation among sheep in the parasitological and inflammatory cell variables, even in lambs of the same breed. In general, animals presenting less inflammatory cells had a larger worm burden, higher fecal egg counts, and larger T. colubriformis worms. The inflammatory cells possibly impaired the parasite's establishment, development, and survival. Universidade Estadual Paulista Instituto de Biociências Depto Parasitologia Universidade Estadual Paulista Instituto de Biociências Depto Parasitologia