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2 results on '"organ perfusion"'

1. The practicalities of establishing a porcine isolated heart model.

Author

Pavey, Warren, Raisis, Anthea, Dunne, Ben, Van Laeken, Els, Jenkinson, Charles, Vincent, Viji, Baird, Peter, Prince, Stuart, Ho, Kwok M., Merry, Christopher, and Gilfillan, Ian

Subjects
*HEART analysis, *ANTHROPOMETRY, *BIOLOGICAL models, *CORONARY disease, *HEART, *HEART transplantation, *HEART failure, *MEDICAL protocols, *MYOCARDIAL reperfusion, *PHLEBOTOMY, *SWINE, *TRANSESOPHAGEAL echocardiography, *CARDIOPLEGIC solutions, *PHARMACODYNAMICS
Abstract

Background: The isolated heart apparatus is over 100 years old, but remains a useful research tool today. While designs of many large animal systems have been described in the literature, trouble-shooting and refining such a model to yield a stable, workable system has not been previously described. This paper outlines the issues, in tabular form, that our group encountered in developing our own porcine isolated heart rig with the aim of assisting other workers in the field planning similar work. The paper also highlights some of the modern applications of the isolated heart apparatus. Methods Landrace pigs (50-80 kg) were used in a pilot project to develop the model. The model was then used in a study examining the effects of various cardioplegic solutions on function after reanimation of porcine hearts. During the two projects, non-protocol issues were documented as well as their solutions. These were aggregated in this paper. Results: Issues faced by the group without explicit literature solutions included pig size selection, animal acclimatisation, porcine transoesophageal echocardiography, cannulation and phlebotomy for cross-clamping, cardioplegia delivery, heart suspension and rig tuning. Conclusion: Prior recognition of issues and possible solutions faced by workers establishing a porcine isolated heart system will speed progress towards a useable system for research. The isolated heart apparatus remains applicable in transplant, ischaemia reperfusion, heart failure and organ preservation research. [ABSTRACT FROM AUTHOR]

Published
2018
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2. Ex vivo perfusion of the swine heart as a method for pre-transplant assessment

Author

S Germscheid, Darren H. Freed, P White, P. Mundt, Ganghong Tian, S. Colah, Ayyaz Ali, Stephen R. Large, and Florian Falter

Subjects
Cardiac function curve, systolic blood pressure, medicine.medical_specialty, Extracorporeal Circulation, organ donor, Swine, ex vivo study, hematocrit, medicine.medical_treatment, Myocardial Reperfusion, Myocardial Reperfusion Injury, electrolyte, Risk Assessment, animal tissue, heart preload, heart function, Internal medicine, Medicine, Animals, Radiology, Nuclear Medicine and imaging, Advanced and Specialized Nursing, Heart transplantation, business.industry, cost effectiveness analysis, Extracorporeal circulation, Heart, General Medicine, Organ Preservation, Tissue Donors, reperfusion, Cardiovascular physiology, Transplantation, Perfusion, preoperative evaluation, Preload, Circulatory system, Cardiology, Heart Transplantation, blood gas, organ perfusion, heart ventricle pressure, brain stem, Cardiology and Cardiovascular Medicine, business, Safety Research
Abstract

We describe a cost-effective, reproducible circuit in a porcine, ex vivo, continuous warm-blood, bi-ventricular, working heart model that has future possibilities for pre-transplant assessment of marginal hearts donated from brain stem dead donors and hearts donated after circulatory determination of death (DCDD). In five consecutive experiments over five days, pressure volume loops were performed. During working mode, the left ventricular end systolic pressure volume relationship (LV ESPVR) was 23.1±11.1 mmHg/ml and the LV preload recruitable stroke work (PRSW) was 67.8±7.2. (Standard PVAN analysis software) (Millar Instruments, Houston, TX, USA) All five hearts were perfused for 219±64 minutes and regained normal cardiac function on the perfusion system.They displayed a significant upward and leftward shift of the end systolic pressure volume relationship, a significant increase in preload recruitable stroke work and minimal stiffness. These hearts could potentially be considered for transplantation. The circuit was effective during reperfusion and working modes whilst proving to be successful in maintaining cardiac function in excess of four hours. Using an autologous prime of approximately 20% haematocrit (Hct), electrolytes and blood gases were easy to control within this period using standard perfusion techniques.

Published
2012

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