1. Nutritional supplementation with transforming growth factor-beta inhibits intestinal adaptation after massive small bowel resection in a rat.
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Sukhotnik, Igor, Mogilner, Jorge, Ben Lulu, Shani, Bashenko, Yulia, Shaoul, Ron, Chemodanov, Elena, Coran, Arnold, Mogilner, Jorge G, and Coran, Arnold G
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TRANSFORMING growth factors-beta , *SMALL intestine surgery , *EPITHELIAL cells , *CELL differentiation , *DIET therapy , *MESSENGER RNA , *LABORATORY rats , *PHYSIOLOGICAL adaptation , *ANIMAL experimentation , *APOPTOSIS , *BIOLOGICAL models , *CELL physiology , *COMPARATIVE studies , *DIETARY supplements , *GROWTH factors , *SMALL intestine , *RESEARCH methodology , *MEDICAL cooperation , *RATS , *RESEARCH , *EVALUATION research , *SHORT bowel syndrome ,DIGESTIVE organ surgery - Abstract
Introduction: Transforming growth factor beta (TGF-β) has been shown to affect epithelial cell differentiation and proliferation through epithelial-mesenchymal and epithelial-immune cell interaction. In the present study, we evaluated the effect of TGF-β2-enriched polymeric diet (Modulen) on enterocyte turnover in a rat model of short bowel syndrome (SBS).Methods: Male rats were divided into four groups: Sham rats and Sham-TGF-β rats underwent bowel transection, and were treated with TGF-β from the 4th postoperative day, SBS rats underwent a 75% bowel resection, and SBS-TGF-β rats underwent bowel resection and were treated with TGF-β-enriched diet similar to Group B. Parameters of intestinal adaptation, enterocyte proliferation and apoptosis were determined on day 15. Real-time PCR was used to determine Bax and Bcl-2 mRNA expression.Results: Treatment of SBS animals with TGF-β2 supplemented diet led to a significant decrease (vs. SBS rats) in bowel weight in ileum (18%, P < 0.05), mucosal DNA content in jejunum (threefold decrease, P < 0.05) and ileum (2.5-fold decrease, P < 0.05), and mucosal protein in jejunum (twofold decrease, P < 0.05) compared to SBS-untreated animals (Group B). Treatment with TGF-β resulted in a mild decrease in enterocyte proliferation in jejunum (25%, P < 0.05) and ileum (18%, P < 0.05). A decreased cell apoptosis in the SBS-TGF-β group was accompanied by a decreased Bax and increased Bcl-2 mRNA expression.Conclusions: In a rat model of SBS, dietary TGF-β inhibits intestinal adaptation. Decreased enterocyte proliferation is responsible for this effect. [ABSTRACT FROM AUTHOR]- Published
- 2011
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