1. Otago Diagnostic Laboratories' (ODL) Method for the detection of beta-lactamases in Enterobacteriaceae.
- Author
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Han CT, Parnell KD, Chiew YF, Lin JY, Turner P, Wilson AS, Davies JD, Robinson JA, de Ruyter AM, Thompson KM, Moodie JA, and Johnstone MC
- Subjects
- Anti-Bacterial Agents pharmacology, Enterobacteriaceae classification, Enterobacteriaceae drug effects, Enterobacteriaceae Infections, Humans, Laboratories, Hospital, New Zealand, Phenotype, beta-Lactamases analysis, Enterobacteriaceae enzymology, Microbial Sensitivity Tests methods, beta-Lactamases biosynthesis
- Abstract
Aim: The rapid evolvement of beta-lactamases in Enterobacteriaceae is an important concern and the clinical microbiology laboratory is required to detect them, where possible, using a rapid, reliable, simple and low cost methodology., Materials and Methods: A disc diffusion method using NCCLS breakpoints, Jarlier's principle and cefoxitin test for AmpC was carried out. It incorporated seven antimicrobial discs in one agar plate: cefotaxime, aztreonam, amoxicillin-clavulanate, ceftazidime, cefpodoxime, cefepime and cefoxitin. NCCLS disc confirmation test for extended-spectrum beta-lactamase (ESBL) was carried out simultaneously., Results: AmpC, ESBL, CTX-M, and K1 were detected using these tests. The prevalence of ESBL was <1% in the hospital., Conclusion: The method is recommended for the phenotypic detection of beta-lactamases in Enterobacteriaceae or for confirmation after the results are obtained by conventional automated systems.
- Published
- 2005
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