1. Comparative assessment of immunochromatographic test kits using somatic antigens from adult Opisthorchis viverrini and IgG and IgG4 conjugates for serodiagnosis of human opisthorchiasis
- Author
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Paul J. Brindley, Lakkhana Sadaow, Pewpan M. Intapan, Rutchanee Rodpai, Hiroshi Yamasaki, Wanchai Maleewong, Wannaporn Ittiprasert, Weeraya Phupiewkham, Oranuch Sanpool, and Victoria H. Mann
- Subjects
medicine.medical_specialty ,Antibodies, Helminth ,Biology ,Gastroenterology ,Opisthorchiasis ,Chromatography, Affinity ,Article ,Serology ,Antigen ,Positive predicative value ,Internal medicine ,parasitic diseases ,medicine ,Animals ,Humans ,Serologic Tests ,Opisthorchis viverrini ,Clonorchis sinensis ,General Veterinary ,Opisthorchis ,fungi ,General Medicine ,medicine.disease ,biology.organism_classification ,Infectious Diseases ,Insect Science ,Antigens, Helminth ,Immunoglobulin G ,Clonorchiasis ,Parasitology ,Somatic antigen - Abstract
Chronic infections of humans with Opisthorchis viverrini and Clonorchis sinensis spanning decades may lead to life-threatening pathology prior to cholangiocarcinoma (CCA), which usually has a poor prognosis. Serological tools can support the parasitological examination in clinical diagnosis and support screening for risk of CCA. We developed novel immunochromatographic test kits using a soluble, somatic tissue extract of adult O. viverrini worms as an antigen and colloidal gold-labeled conjugates of IgG and IgG4 antibodies, and evaluated the diagnostic values of both the OvSO-IgG and OvSO-IgG4 kits. For diagnosis of human opisthorchiasis individually, the diagnostic sensitivity, specificity, and positive and negative predictive values with 95% confidence intervals in the OvSO-IgG kit were 86.6% (78.9–92.3), 89.5% (84.2–93.5), 82.9% (74.8–89.2), and 91.9% (87.0–95.4), respectively, while the 75% (65.9–82.7), 98.4% (95.5–99.7), 96.6% (90.3–99.3), and 87% (81.7–91.2), respectively, for the OvSO-IgG4 kit at the prevalence of infection of 37.1%. Twenty-three (76.7%) and 14 (46.7%) of 30 clonorchiasis sera showed positive reactivity with the OvSO-IgG and OvSO-IgG4 kits, respectively. There was 84.1% (κ-value = 0.649) concordance between the two kits, which was statistically significant (p
- Published
- 2021