6 results on '"Brindley, Paul J."'
Search Results
2. Decreased risk of cholangiocarcinogenesis following repeated cycles of Opisthorchis viverrini infection-praziquantel treatment: Magnetic Resonance Imaging (MRI) and histopathological study in a hamster model.
- Author
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Hanpanich P, Laha T, Sripa B, Mairiang E, Sereerak P, Upontain S, Tangkawattana P, Brindley PJ, and Tangkawattana S
- Subjects
- Animals, Cricetinae, Humans, Magnetic Resonance Imaging, Male, Mesocricetus, Opisthorchiasis complications, Opisthorchiasis parasitology, Risk, Anthelmintics therapeutic use, Bile Duct Neoplasms parasitology, Carcinogenesis drug effects, Cholangiocarcinoma parasitology, Opisthorchiasis drug therapy, Opisthorchis physiology, Praziquantel therapeutic use
- Abstract
It has been suggested that repeated infection of Opisthorchis viverrini followed by repeated treatment with praziquantel (PZQ) increases risk of development of cholangiocarcinoma (CCA). Evidence for the prediction has accumulated based on findings of indirect approaches involving molecular changes and epidemiological trends. By contrast, here we directly monitored the impact of repeated liver fluke infection and treatment with PZQ on cholangiocarcinogenesis in a rodent model of human opisthorchiasis, using magnetic resonance imaging (MRI) and histopathology. Twenty five Syrian golden hamsters were assigned to five treatment groups: 1) infection with O. viverrini (OV group), 2) treatment with the carcinogen N-nitrosodimethylamine (NDMA) at 12.5ppm (DMN), 3) O. viverrini infection in tandem with NDMA (OD), 4) O. viverrini infection, NDMA, and treatment with PZQ (ODP), and 5) uninfected, untreated control. The repeated infections were established by intragastric inoculation of 50 metacercariae of O. viverrini to the OV, OD and ODP hamsters at weeks 0, 5 and 10. PZQ at 300mg/kg body weight was given to each hamster of the ODP group on weeks 4, 9 and 13 (four weeks after each infection). Imaging by MRI was undertaken on weeks 5, 10 and 14 (i.e. one week after each PZQ treatment). MRI revealed that the ODP hamsters did not develop CCA, whereas necropsy at week 40 revealed CCA in hamsters of the OD and DMN groups. Findings for histopathology and for proliferating cell nuclear antigen index conformed to the MRI findings. In overview, and notwithstanding that the immune response of individual hosts may play roles in cholangiocarcinogenesis, three cycles of the infection with O. viverrini followed treatment of the infection with PZQ did not increase the risk of bile duct cancer in this hamster model of liver fluke infection-induced CCA., (Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2017
- Full Text
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3. Ultrasonography assessment of hepatobiliary abnormalities in 3359 subjects with Opisthorchis viverrini infection in endemic areas of Thailand.
- Author
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Mairiang E, Laha T, Bethony JM, Thinkhamrop B, Kaewkes S, Sithithaworn P, Tesana S, Loukas A, Brindley PJ, and Sripa B
- Subjects
- Adult, Animals, Biliary Tract diagnostic imaging, Biliary Tract pathology, Biliary Tract Diseases complications, Biliary Tract Diseases epidemiology, Biliary Tract Diseases pathology, Cholangiocarcinoma complications, Cholangiocarcinoma epidemiology, Cholangiocarcinoma pathology, Cross-Sectional Studies, Feces parasitology, Female, Gallbladder diagnostic imaging, Gallbladder pathology, Gallbladder Diseases complications, Gallbladder Diseases epidemiology, Gallbladder Diseases pathology, Humans, Liver diagnostic imaging, Liver pathology, Liver Diseases complications, Liver Diseases epidemiology, Liver Diseases pathology, Male, Middle Aged, Opisthorchiasis complications, Opisthorchiasis epidemiology, Opisthorchiasis pathology, Opisthorchis physiology, Prevalence, Risk Factors, Thailand epidemiology, Ultrasonography, Young Adult, Biliary Tract Diseases diagnostic imaging, Cholangiocarcinoma diagnostic imaging, Gallbladder Diseases diagnostic imaging, Liver Diseases diagnostic imaging, Opisthorchiasis diagnostic imaging
- Abstract
A cross sectional study on hepatobiliary abnormalities in opisthorchiasis was performed in 8936 males and females aged from 20 to 60 years from 90 villages of Khon Kaen province, Northeast Thailand. All were stool-examined for Opisthorchis viverrini infection by standard quantitative formalin/ethyl acetate concentration technique. Of these, 3359 participants with stool egg positive underwent ultrasonography of the upper abdomen. The hepatobiliary abnormalities detected by ultrasound are described here. This study found a significantly higher frequency of advanced periductal fibrosis in persons with chronic opisthorchiasis (23.6%), particularly in males. Risks of the fibrosis included intensity of infection, and age younger than 30 years. Height of left lobe of the liver, cross-section of the gallbladder dimensions post fatty meal, sludge, and, interestingly, intrahepatic duct stones were significantly associated with the advanced periductal fibrosis. Eleven suspected cholangiocarcinoma (CCA) cases were observed. This study emphasizes the current status of high O. viverrini infection rate and the existence of hepatobiliary abnormalities including suspected CCA in opisthorchiasis endemic areas of Thailand., (Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
4. Banking on the future: biobanking for "omics" approaches to biomarker discovery for Opisthorchis-induced cholangiocarcinoma in Thailand.
- Author
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Mulvenna J, Yonglitthipagon P, Sripa B, Brindley PJ, Loukas A, and Bethony JM
- Subjects
- Animals, Bile Duct Neoplasms parasitology, Bile Duct Neoplasms pathology, Bile Ducts, Intrahepatic pathology, Biomarkers, Tumor blood, Biomarkers, Tumor classification, Cell Line, Cholangiocarcinoma parasitology, Cholangiocarcinoma pathology, Genomics methods, Humans, Liver Diseases, Parasitic diagnosis, Liver Diseases, Parasitic pathology, Metabolomics methods, Opisthorchiasis complications, Opisthorchiasis parasitology, Opisthorchiasis pathology, Opisthorchis physiology, Risk Assessment, Thailand, Bile Duct Neoplasms diagnosis, Biomarkers, Tumor analysis, Cholangiocarcinoma diagnosis, Opisthorchiasis diagnosis, Proteomics methods
- Abstract
Cholangiocarcinoma (CCA)--bile duct cancer--is associated with late presentation, poses challenges for diagnosis, and has high mortality. These features t highlight the desperate need for biomarkers than can be measured early and in accessible body fluids such as plasma of people at risk for developing this lethal cancer. In this manuscript, we address previous limitations in the discovery stage of biomarker(s) for CCA and indicate how new generation of "omics" technologies could be used for biomarker discovery in Thailand. A key factor in the success of this biomarker program for CCA is the combination of cutting edge technology with strategic sample acquisition by a biorepositories., (Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2012
- Full Text
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5. Evaluation of liver fluke recombinant cathepsin B-1 protease as a serodiagnostic antigen for human opisthorchiasis
- Author
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Sripa, Jittiyawadee, Brindley, Paul J., Sripa, Banchob, Loukas, Alex, Kaewkes, Sasithorn, and Laha, Thewarach
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LIVER flukes , *PEPTIDES , *RECOMBINANT proteins , *OPISTHORCHIASIS , *SERODIAGNOSIS , *PROTEOLYTIC enzymes , *CYSTEINE proteinases - Abstract
Abstract: A cathepsin B-like cysteine protease belonging to family C1 is abundantly expressed in the transcriptome and proteome of the carcinogenic liver fluke of humans, Opisthorchis viverrini. This enzyme is present in excretory/secretory (ES) products released by parasites cultured in vitro. This study evaluated the performance of recombinant O. viverrini cathepsin B1 (rOv-CB-1) as an antigen for immunodiagnosis of opisthorchiasis. The full length Ov-CB-1 cDNA was cloned and recombinant protein was produced in catalytically active form in Pichia pastoris. The recombinant Ov-CB-1 (rOv-CB-1) was affinity purified via nickel-NTA chromatography and tested in enzyme-linked immunosorbent assays (ELISA) with human sera from an opisthorchiasis endemic area. Sera from egg-positive O. viverrini infections produced a strong IgG antibody response to rOv-CB-1 both in ELISA and immunoblot analysis. The sensitivity and specificity of the ELISA test was 67% and 81%, respectively. These findings support the feasibility of using recombinant Ov-CB-1 in ELISA for the serodiagnosis of human opisthorchiasis. [Copyright &y& Elsevier]
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- 2012
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6. Cloning, expression, and characterization of a novel Opisthorchis viverrini calcium-binding EF-hand protein
- Author
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Senawong, Gulsiri, Laha, Thewarach, Loukas, Alex, Brindley, Paul J., and Sripa, Banchob
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OPISTHORCHIASIS , *CLONING , *GENE expression , *CALCIUM-binding proteins , *COMPLEMENTARY DNA , *CHOLANGIOCARCINOMA , *TREMATODA - Abstract
Abstract: A novel 22.8kDa of Opisthorchis viverrini (Ov) calcium-binding EF-hand protein (Ov CaBP) was identified and isolated from an immunoscreening of the adult stage Ov cDNA library by using a human cholangiocarcinoma (CCA) serum. This protein was related to other calcium-binding proteins and conserved among the trematodes. Ov CaBP shared 98% amino acid identity to 22.8kDa of Clonorchis sinensis CaBP and both were classified as a new group of CaBP EF-hand protein by multiple sequence alignment and phylogenetic tree analysis. The open reading frame of Ov CaBP was 585bp which encoded for 194 amino acids. The N-terminal part is composed of two calcium-binding EF-hand motifs whereas the C-terminal part contains a dynein light chain motif (DLC). In addition, transcription analysis by RT-PCR revealed that it was constitutively transcribed in all stages, including metacercariae, juvenile, and adult. Furthermore, recombinant Ov CaBP protein (rOv CaBP) was expressed as a soluble protein and antibody generated against this rOv CaBP protein was capable of detecting Ov CaBP in the Ov somatic extracts but not in Ov ES products. This anti-rOv CaBP serum was also used to localize Ov CaBP in Ov infected hamster''s liver sections which the distribution of Ov CaBP was located in gut epithelium, miracidia in eggs and slightly in parenchyma. Moreover, rOv CaBP protein showed a calcium-binding property in non-denaturing gel mobility shift assay. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
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