1. Anti-α-Gal antibodies detected by novel neoglycoproteins as a diagnostic tool for Old World cutaneous leishmaniasis caused byLeishmania major
- Author
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Katja Michael, Nathaniel S. Schocker, Roger A. Ashmus, Mina Mesri, Igor C. Almeida, Krishanthi Subramaniam, Matthew S. Anderson, Alba Montoya, Waleed S. Al-Salem, Victoria M. Austin, and Alvaro Acosta-Serrano
- Subjects
Adult ,Male ,0301 basic medicine ,Adolescent ,Antibodies, Helminth ,Leishmaniasis, Cutaneous ,Heterologous ,Epitopes ,Middle East ,Young Adult ,03 medical and health sciences ,Cutaneous leishmaniasis ,medicine ,Animals ,Humans ,Parasite hosting ,Leishmania major ,Biological Specimen Banks ,Glycoproteins ,biology ,Middle Aged ,Standard methods ,biology.organism_classification ,medicine.disease ,Virology ,030104 developmental biology ,Infectious Diseases ,Old World cutaneous leishmaniasis ,Antigens, Helminth ,Area Under Curve ,biology.protein ,Regression Analysis ,Female ,Animal Science and Zoology ,Parasitology ,Antibody - Abstract
Outbreaks of Old World cutaneous leishmaniasis (CL) have significantly increased due to the conflicts in the Middle East, with most of the cases occurring in resource-limited areas such as refugee settlements. The standard methods of diagnosis include microscopy and parasite culture, which have several limitations. To address the growing need for a CL diagnostic that can be field applicable, we have identified five candidate neoglycoproteins (NGPs): Galα (NGP3B), Galα(1,3)Galα (NGP17B), Galα(1,3)Galβ (NGP9B), Galα(1,6)[Galα(1,2)]Galβ (NGP11B), and Galα(1,3)Galβ(1,4)Glcβ (NGP1B) that are differentially recognized in sera from individuals withLeishmania majorinfection as compared with sera from heterologous controls. These candidates contain terminal, non-reducing α-galactopyranosyl (α-Gal) residues, which are known potent immunogens to humans. Logistic regression models found that NGP3B retained the best diagnostic potential (area under the curve from receiver-operating characteristic curve = 0.8). Our data add to the growing body of work demonstrating the exploitability of the human anti-α-Gal response in CL diagnosis.
- Published
- 2018
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