1. Cartilage Oligomeric Matrix Protein-Angiopoietin-1 Has a Protective Effect of Vascular Endothelial Barrier in Rat With Acute Necrotizing Pancreatitis
- Author
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Ping-Ting Kong, Ji-yun Tian, Jia-min Tu, Hong-chang Li, Li-yun Pan, Pei-Hao Yin, Jin-kun Xie, Chen Teng, Ya-feng Chen, Wen Peng, Yi-Jun Cao, Xin-juan Fan, and Dian-xu Feng
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,Pathology ,Time Factors ,Angiogenesis ,Recombinant Fusion Proteins ,Endocrinology, Diabetes and Metabolism ,Anti-Inflammatory Agents ,Neovascularization, Physiologic ,Vascular permeability ,Capillary Permeability ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,Endocrinology ,Western blot ,Internal medicine ,Angiopoietin-1 ,Internal Medicine ,medicine ,Animals ,RNA, Messenger ,Pancreas ,Evans Blue ,Cartilage oligomeric matrix protein ,Endothelin-1 ,Hepatology ,biology ,medicine.diagnostic_test ,Pancreatitis, Acute Necrotizing ,C-reactive protein ,Endothelin 1 ,Extravasation ,Up-Regulation ,Disease Models, Animal ,C-Reactive Protein ,030104 developmental biology ,chemistry ,Amylases ,cardiovascular system ,biology.protein ,Angiogenesis Inducing Agents ,hormones, hormone substitutes, and hormone antagonists - Abstract
OBJECTIVE To investigate the protective effect of angiopoietin-1 (Ang-1) from capillary endothelial damage in rats with acute necrotizing pancreatitis (ANP). METHODS 96 male Sprague-Dawley rats were randomly averaged and divided into control group, ANP group, Si-Ang-1 group, and COMP (cartilage oligomeric matrix protein)-Ang-1 group. Animals were killed at 6, 12, and 24 hours after molding. Levels of serum amylase, porcine endothelin 1, C-reactive protein, and Ang-1 were detected; histopathological changes in the pancreas were observed; capillary permeability and Ang-1 expression of the pancreatic tissue were detected by Evans Blue extravasation assay, immunohistochemistry, Western blot, and quantitative polymerase chain reaction. RESULTS (1) Levels of serum amylase, C-reactive protein, and porcine endothelin-1 increased and level of Ang-1 decrease in the ANP group and Si-Ang-1 group compared with the control group, whereas COMP-Ang-1 group could improve the changes. (2) The order of pancreas pathological changes (mild to severe) is: control group, COMP-Ang-1 group, ANP group, and Si-Ang-1 group. (3) Capillary permeability of the pancreatic tissue in the COMP-Ang-1 group was lower than that in the ANP group. (4) Ang-1 mRNA and protein expression in the COMP-Ang-1 group was significantly higher than in the ANP group. CONCLUSIONS COMP-Ang-1 can upregulate the expression of Ang-1 protein to promote angiogenesis and improve early inflammatory and pathological damage in ANP group.
- Published
- 2016