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Abstract: The natural abundance of C3 and C4 plants is affected by multiple environmental factors including temperature, moisture balance and atmospheric pCO2. The relative importance of these factors is a subject of considerable debate, and may vary in different natural ecosystems. Previous studies generally focus on single loess sequences in the Chinese Loess Plateau, and conflicting conclusions on C4/C3 have been reached when studying carbon isotope ratios in carbonate and organic matter. In this paper we report a comprehensive carbon isotopic characterization of total organic carbon (TOC) and individual higher plant leaf waxes from five loess sequences spanning the last 35 ka from the Chinese Loess Plateau (CLP). The five coring sites encompass large gradients of annual mean temperature (9.2–13.9 °C) and precipitation (402–673 mm), allowing us to assess the controlling mechanisms on C4/C3 plant ratios. Glacial–interglacial sequences provide carbon isotope data for comparison with other climatic and environmental proxies such as lithology and magnetic susceptibility. Our results demonstrate that increased C4/C3 ratios are positively correlated with higher temperature and increased summer rainfall which characterize stronger summer monsoon in all five sites. We conclude C4 abundance increases from the last glacial to the Holocene in response to greater monsoon activity and that the C4 expression is suppressed in the cold and drier intervals. [Copyright &y& Elsevier]