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Background: Urinary tract infections (UTIs) in women are one of the most prevalent infections occurring at various stages of life. UTI represents one of the most common, challenging and widely misunderstood infectious diseases among women as outpatient and nosocomial infections leading to morbidity. Aim: The objective of our study was to determine the knowledge, attitude and practices of urinary tract infection among Pakistani diabetic females and create awareness regarding its preventive measures. Study type, settings & duration: This observational study was carried out at South East Hospital and Research Center, Islamabad over a period of six month from February to August 2022. Methodology: The non probability convenient sampling method technique was used. Data was collected through pre-structured proforma and entered in SPSS version 25.0. Questionnaire written in English and local language was handed over to each diabetic patient. All the responses were collected and the same was entered in the software for analysis. Results: The mean age of patients was 52 years, Weight 70kg, mean duration of diabetes 8 years and random blood glucose level was 268.63 mg/dL. Total of 36.3% diabetic females had knowledge that they are at increased risk of developing UTI. Only 24.7% knew that interrupted urinary stream can be a symptom of UTI, 27.9% knew about fever and 12.4% knew about abdominal pain. Only 7.1% diabetic females knew that incontinence of urine can be its symptom. Almost 60% of the patients knew about the importance of timely treatment of UTI. Conclusion: Urinary tract infection is a common infection among diabetic females in Pakistan. Our study concluded that most of the educated women had adequate knowledge regarding UTI and they also had a positive attitude towards the symptoms and management of UTI. We need to design awareness programs for uneducated females regarding UTI. [ABSTRACT FROM AUTHOR]