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Start Over Author "Scognamiglio I" Journal oxidative medicine and cellular longevity
3 results on '"Scognamiglio I"'

1. Short-Term Diet and Moderate Exercise in Young Overweight Men Modulate Cardiocyte and Hepatocarcinoma Survival by Oxidative Stress

Author

Giuseppe A. Martin, Michele Caraglia, Ilaria Scognamiglio, Giovanni Messina, Pasquale Sperlongano, Marina Porcelli, Paola Stiuso, Angela Lombardi, Marcellino Monda, Monda, Marcellino, Messina, G, Scognamiglio, I, Lombardi, A, Martin, Ga, Sperlongano, Pasquale, Porcelli, Marina, Caraglia, Michele, and Stiuso, Paola

Subjects
Male, Aging, Time Factors, HSP27 Heat-Shock Proteins, Blood lipids, Overweight, Mitochondrion, medicine.disease_cause, Biochemistry, chemistry.chemical_compound, Superoxides, Diet, Fat-Restricted, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, lcsh:Cytology, General Medicine, Hep G2 Cells, Lipids, Mitochondria, moderate exercise, Short-term diet, medicine.symptom, Liver cancer, Research Article, Adult, medicine.medical_specialty, Article Subject, Adolescent, Biology, Nitric Oxide, Nitric oxide, Cell Line, Young Adult, Internal medicine, medicine, Aerobic exercise, Animals, Humans, HSP90 Heat-Shock Proteins, lcsh:QH573-671, Exercise, Cell Proliferation, Cell Biology, medicine.disease, Culture Media, Rats, Oxidative Stress, Endocrinology, chemistry, hepatocarcinoma, Metabolic syndrome, Oxidative stress
Abstract

The present study was designed to evaluate the effects of diet lifestyle on extending lifespan and reducing liver cancer risk. Young overweight men(n=20), without metabolic syndrome, were placed in a 3-week residential program on a low-fat diet and moderate aerobic exercise. In each subject, pre- and postintervention fasting blood were collected for evaluating levels of serum lipids, and oxidative stress markers. Using subject sera and cardiomyocyte (H9C2) culture systems, we measured heat shock protein 27 and 90 expression, lipid accumulation, and oxidative stress marker levels. After 3-weeks of diet, significant reductions(P<0.05)in body mass index, serum lipids and lipid ratios, and oxidative markers were recorded.In vitro, we observed that the addition of postintervention sera increased H9C2 cell number and reduced HSP27 and 90 expression, mitochondrial superoxide anion, and lipid accumulation with a parallel increase in nitric oxide (NO) production (allP<0.01). At the same time, postintervention sera decreased human liver hepatocellular carcinoma cell line (HepG-2) proliferation, lipid accumulation, oxidative stress, and extracellular-signal-regulated kinases (ERK1/2) activity. Lifestyle modification in young overweight men, without metabolic syndrome, could ameliorate cardiocyte survival and reduce hepatocellular carcinoma cell proliferation.

Published
2014

2. Short-term diet and moderate exercise in young overweight men modulate cardiocyte and hepatocarcinoma survival by oxidative stress.

Author

Monda M, Messina G, Scognamiglio I, Lombardi A, Martin GA, Sperlongano P, Porcelli M, Caraglia M, and Stiuso P

Subjects
Adolescent, Adult, Animals, Cell Line, Cell Proliferation drug effects, Culture Media chemistry, Culture Media pharmacology, HSP27 Heat-Shock Proteins metabolism, HSP90 Heat-Shock Proteins metabolism, Hep G2 Cells, Humans, Lipids blood, Male, Mitochondria metabolism, Mitogen-Activated Protein Kinase 1 metabolism, Mitogen-Activated Protein Kinase 3 metabolism, Nitric Oxide metabolism, Rats, Superoxides metabolism, Time Factors, Young Adult, Diet, Fat-Restricted, Exercise, Overweight, Oxidative Stress
Abstract

The present study was designed to evaluate the effects of diet lifestyle on extending lifespan and reducing liver cancer risk. Young overweight men (n = 20), without metabolic syndrome, were placed in a 3-week residential program on a low-fat diet and moderate aerobic exercise. In each subject, pre- and postintervention fasting blood were collected for evaluating levels of serum lipids, and oxidative stress markers. Using subject sera and cardiomyocyte (H9C2) culture systems, we measured heat shock protein 27 and 90 expression, lipid accumulation, and oxidative stress marker levels. After 3-weeks of diet, significant reductions (P < 0.05) in body mass index, serum lipids and lipid ratios, and oxidative markers were recorded. In vitro, we observed that the addition of postintervention sera increased H9C2 cell number and reduced HSP27 and 90 expression, mitochondrial superoxide anion, and lipid accumulation with a parallel increase in nitric oxide (NO) production (all P < 0.01). At the same time, postintervention sera decreased human liver hepatocellular carcinoma cell line (HepG-2) proliferation, lipid accumulation, oxidative stress, and extracellular-signal-regulated kinases (ERK1/2) activity. Lifestyle modification in young overweight men, without metabolic syndrome, could ameliorate cardiocyte survival and reduce hepatocellular carcinoma cell proliferation.

Published
2014
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3. Serum oxidative stress markers and lipidomic profile to detect NASH patients responsive to an antioxidant treatment: a pilot study.

Author

Stiuso P, Scognamiglio I, Murolo M, Ferranti P, De Simone C, Rizzo MR, Tuccillo C, Caraglia M, Loguercio C, and Federico A

Subjects
Adult, Antioxidants pharmacology, Case-Control Studies, Catalase blood, Female, Hep G2 Cells, Humans, Male, Nitric Oxide blood, Non-alcoholic Fatty Liver Disease drug therapy, Phosphatidylcholines metabolism, Pilot Projects, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Superoxide Dismutase blood, Thiobarbituric Acid Reactive Substances metabolism, Antioxidants therapeutic use, Biomarkers blood, Lipid Metabolism drug effects, Metabolomics, Non-alcoholic Fatty Liver Disease blood, Non-alcoholic Fatty Liver Disease diagnosis, Oxidative Stress drug effects
Abstract

Liver steatosis can evolve to steatohepatitis (NASH) through a series of biochemical steps related to oxidative stress in hepatocytes. Antioxidants, such as silybin, have been proposed as a treatment of patients with nonalcoholic fatty liver disease (NAFLD) and NASH. In this study, we evaluated, in patients with histologically documented NASH, the oxidant/antioxidant status and lipid "fingerprint" in the serum of NASH patients, both in basal conditions and after 12 months of treatment with silybin-based food integrator Realsil (RA). The oxidant/antioxidant status analysis showed the presence of a group of patients with higher basal severity of disease (NAS scores 4.67 ± 2.5) and a second group corresponding to borderline NASH (NAS scores = 3.8 ± 1.5). The chronic treatment with RA changed the NAS score in both groups that reached the statistical significance only in group 2, in which there was also a significant decrease of serum lipid peroxidation. The lipidomic profile showed a lipid composition similar to that of healthy subjects with a restoration of the values of free cholesterol, lysoPC, SM, and PC only in group 2 of patients after treatment with RA. Conclusion. These data suggest that lipidomic and/or oxidative status of serum from patients with NASH could be useful as prognostic markers of response to an antioxidant treatment.

Published
2014
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