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Your search keyword '"Frazzitta, Anna"' showing total 3 results
Start Over Author "Frazzitta, Anna" Journal oxidative medicine and cellular longevity
3 results on '"Frazzitta, Anna"'

2. Indicaxanthin from Opuntia ficus indica (L. Mill) Inhibits Oxidized LDL-Mediated Human Endothelial Cell Dysfunction through Inhibition of NF-κB Activation

Author

Anna Frazzitta, Mario Allegra, Alessandro Attanzio, Rosalia Busà, Maria A. Livrea, Luisa Tesoriere, Attanzio, Alessandro, Frazzitta, Anna, Busa, Rosalia, Tesoriere, Luisa, Livrea, Maria A., and Allegra, Mario

Subjects
0301 basic medicine, Aging, Article Subject, Transcription, Genetic, Cell Survival, Pyridine, Human Umbilical Vein Endothelial Cell, 030204 cardiovascular system & hematology, Pharmacology, Biochemistry, Umbilical vein, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Settore BIO/10 - Biochimica, medicine, RNA, Messenger, Reactive Nitrogen Specie, Endothelial dysfunction, lcsh:QH573-671, Cytotoxicity, Reactive nitrogen species, chemistry.chemical_classification, Reactive oxygen species, lcsh:Cytology, NF-kappa B, Opuntia, Hydrogen Peroxide, Cell Biology, General Medicine, NFKB1, medicine.disease, Settore CHIM/08 - Chimica Farmaceutica, Up-Regulation, Lipoproteins, LDL, Endothelial stem cell, 030104 developmental biology, chemistry, Cell Adhesion Molecule, Betaxanthin, Thiobarbituric Acid Reactive Substance, Reactive Oxygen Specie, Oxidation-Reduction, Indicaxanthin, ATP Binding Cassette Transporter 1, Human
Abstract

Oxidized low-density lipoproteins (oxLDL) play a pivotal role in the etiopathogenesis of atherosclerosis through the activation of inflammatory signaling events eventually leading to endothelial dysfunction and senescence. In the present work, we investigated the effects of indicaxanthin, a bioavailable, redox-modulating phytochemical from Opuntia ficus indica fruits, with anti-inflammatory activity, against oxLDL-induced endothelial dysfunction. Human umbilical vein cord cells (HUVEC) were stimulated with human oxLDL, and the effects of indicaxanthin were evaluated in a range between 5 and 20 μM, consistent with its plasma level after a fruit meal (7 μM). Pretreatment with indicaxanthin significantly and concentration-dependently inhibited oxLDL-induced cytotoxicity; ICAM-1, VCAM-1, and ELAM-1 increase; and ABC-A1 decrease of both protein and mRNA levels. From a mechanistic perspective, we also provided evidence that the protective effects of indicaxanthin were redox-dependent and related to the pigment efficacy to inhibit NF-κB transcriptional activity. In conclusion, here we demonstrate indicaxanthin as a novel, dietary phytochemical, able to exert significant protective vascular effects in vitro, at nutritionally relevant concentrations.

Published
2019

3. Indicaxanthin from Opuntia ficus indica (L. Mill) Inhibits Oxidized LDL-Mediated Human Endothelial Cell Dysfunction through Inhibition of NF- κ B Activation.

Author

Attanzio A, Frazzitta A, Busa' R, Tesoriere L, Livrea MA, and Allegra M

Subjects
ATP Binding Cassette Transporter 1 genetics, ATP Binding Cassette Transporter 1 metabolism, Betaxanthins chemistry, Cell Adhesion Molecules genetics, Cell Adhesion Molecules metabolism, Cell Survival drug effects, Human Umbilical Vein Endothelial Cells drug effects, Human Umbilical Vein Endothelial Cells metabolism, Humans, Hydrogen Peroxide metabolism, Oxidation-Reduction, Pyridines chemistry, RNA, Messenger genetics, RNA, Messenger metabolism, Reactive Nitrogen Species metabolism, Reactive Oxygen Species metabolism, Thiobarbituric Acid Reactive Substances, Transcription, Genetic drug effects, Up-Regulation drug effects, Up-Regulation genetics, Betaxanthins pharmacology, Human Umbilical Vein Endothelial Cells pathology, Lipoproteins, LDL adverse effects, NF-kappa B metabolism, Opuntia chemistry, Pyridines pharmacology
Abstract

Oxidized low-density lipoproteins (oxLDL) play a pivotal role in the etiopathogenesis of atherosclerosis through the activation of inflammatory signaling events eventually leading to endothelial dysfunction and senescence. In the present work, we investigated the effects of indicaxanthin, a bioavailable, redox-modulating phytochemical from Opuntia ficus indica fruits, with anti-inflammatory activity, against oxLDL-induced endothelial dysfunction. Human umbilical vein cord cells (HUVEC) were stimulated with human oxLDL, and the effects of indicaxanthin were evaluated in a range between 5 and 20 μ M, consistent with its plasma level after a fruit meal (7 μ M). Pretreatment with indicaxanthin significantly and concentration-dependently inhibited oxLDL-induced cytotoxicity; ICAM-1, VCAM-1, and ELAM-1 increase; and ABC-A1 decrease of both protein and mRNA levels. From a mechanistic perspective, we also provided evidence that the protective effects of indicaxanthin were redox-dependent and related to the pigment efficacy to inhibit NF- κ B transcriptional activity. In conclusion, here we demonstrate indicaxanthin as a novel, dietary phytochemical, able to exert significant protective vascular effects in vitro , at nutritionally relevant concentrations.

Published
2019
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