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Objective: Cationic tantalum oxide nanoparticles (Ta 2 O 5 -cNPs), as a newly introduced contrast agent for computed tomography of cartilage, offer quantitative evaluation of proteoglycan (PG) content and biomechanical properties. However, knowledge on the depth-wise impact of cartilage constituents on nanoparticle diffusion, particularly the influence of the collagen network, is lacking. In this study, we aim to establish the depth-dependent relationship between Ta 2 O 5 -cNP diffusion and cartilage constituents (PG content, collagen content and network architecture)., Methods: Osteochondral samples (n = 30) were harvested from healthy equine stifle joints (N = 15) and the diffusion of 2.55 nm diameter cationic Ta 2 O 5 -cNPs into the cartilage was followed with micro computed tomography (µCT) imaging for up to 96 hours. The diffusion-related parameters, Ta 2 O 5 -cNP maximum partition (P max ) and diffusion time constant, were compared against biomechanical and depth-wise structural properties. Biomechanics were assessed using stress-relaxation and sinusoidal loading protocols, whereas PG content, collagen content and collagen network architecture were determined using digital densitometry, Fourier-transform infrared spectroscopy and polarized light microscopy, respectively., Results: The P max correlates with the depth-wise distribution of PGs (bulk Spearman's ρ = 0.87, p < 0.001). More open collagen network architecture at the superficial zone enhances intake of Ta 2 O 5 -cNPs, but collagen content overall decreases the intake. The P max values correlate with the equilibrium modulus (ρ = 0.80, p < 0.001) of articular cartilage., Conclusion: This study establishes the feasibility of Ta 2 O 5 -cNPs for the precise and comprehensive identification of biomechanical and structural changes in articular cartilage via contrast-enhanced µCT., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)